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Literature summary for 2.7.1.35 extracted from
- Catalytic and regulatory roles of species involved in metal-nucleotide equilibriums in human pyridoxal kinase (2013), Biometals, 26, 805-812.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | ATP4- and HATP3- do not affect the enzyme activity. Free Mg2+ does not inhibit enzyme activity | Homo sapiens | |
| pyridoxal | substrate inhibition | Homo sapiens |  |
| Zn2+ | - |
Homo sapiens | |
| ZnATP2- | weak substrate inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
ATP | in the presence of Zn2+, at pH 6.5 and 37°C | Homo sapiens | |
| 0.49 | - |
pyridoxal | at pH 6.5 and 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | activator at low concentrations (0.019 mM optimal concentration), most effective divalent cation for catalysis | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyridoxal | Homo sapiens | - |
ADP + pyridoxal 5'-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyridoxal | - |
Homo sapiens | ADP + pyridoxal 5'-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLK | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
ATP | in the presence of Zn2+, at pH 6.5 and 37°C | Homo sapiens | |
| 3.2 | - |
pyridoxal | at pH 6.5 and 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
at pH 6.5 and 37°C | Homo sapiens | Zn2+ | |
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