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acetyl-CoA + H2O + oxaloacetate = citrate + CoA
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
mechanism
-
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
active site His320
-
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
The stereospecificity of this enzyme is opposite to that of EC 2.3.3.4, citrate (Re)-synthase, which is found in some anaerobes
-
-
-
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
high-level ab initio quantum mechanical/molecular mechanical modelling of citryl-CoA formation in citrate synthase reveals that an arginine residue acts as the proton donor
-
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acetyl-CoA + H2O + oxaloacetate
citrate + CoA
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
Substrates: -
Products: -
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: entry step to tricarboxylic acid cycle
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: key enzyme of tricarboxylic acid cycle
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: intermediate citryl-CoA
Products: -
r
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: entry step to tricarboxylic acid cycle
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: key enzyme of tricarboxylic acid cycle
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
-
Substrates: -
Products: -
?
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2-oxoglutarate
-
no inhibition
3,3-thiodipropionate
substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive
3,3-thiodipropionic acid
competitive inhibition versus acetyl-CoA and versus oxaloacetate
3-phosphonopropionic acid
competitive inhibition versus acetyl-CoA and versus oxaloacetate
3-phosphopropionate
substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive
amphetamine
-
valproate reverses and lithium prevents the enzyme inhibition
Dihydroxyfumarate
substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive
dihydroxyfumaric acid
competitive inhibition versus acetyl-CoA and versus oxaloacetate
DL-malate
substrate acetyl-CoA, mixed type inhibition; substrate oxaloacetate, mixed type inhibition
DL-malic acid
mixed type inhibition versus acetyl-CoA and versus oxaloacetate
Maleate
substrate acetyl-CoA, mixed type inhibition; substrate oxaloacetate, mixed type inhibition
Maleic acid
mixed type inhibition versus acetyl-CoA and versus oxaloacetate
Ouabain
-
activity was inhibited immediately after the administration of ouabain in the prefrontal cortex at the doses of 0.01 mM and 0.1 mM
potassium hydroxycitrate
competitive inhibition versus acetyl-CoA and versus oxaloacetate; substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive
propionate
competitive inhibition versus oxaloacetate, mixed type inhibition versus acetyl-CoA; substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive
ATP
-
no inhibition
ATP
-
noncompetitive against acetyl-CoA
NADH
-
no effect
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0.005
acetyl-CoA
-
wild-type
0.0077
acetyl-CoA
wild-type, pH 8.0, temperature not specified in the publication
0.0077
acetyl-CoA
pH 8.0, temperature not specified in the publication, wild-type enzyme
0.0104
acetyl-CoA
mutant A348G, pH 8.0, temperature not specified in the publication
0.0104
acetyl-CoA
pH 8.0, temperature not specified in the publication, mutant enzyme G348G
0.089
acetyl-CoA
-
mutant H320G
0.0036
oxaloacetate
wild-type, pH 8.0, temperature not specified in the publication
0.0036
oxaloacetate
pH 8.0, temperature not specified in the publication, wild-type enzyme
0.0057
oxaloacetate
mutant A348G, pH 8.0, temperature not specified in the publication
0.0057
oxaloacetate
pH 8.0, temperature not specified in the publication, mutant enzyme G348G
0.0059
oxaloacetate
-
wild-type
0.043
oxaloacetate
-
mutant H320G
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117
acetyl-CoA
mutant A348G, pH 8.0, temperature not specified in the publication
117
acetyl-CoA
pH 8.0, temperature not specified in the publication, mutant enzyme G348G
260
acetyl-CoA
wild-type, pH 8.0, temperature not specified in the publication
260
acetyl-CoA
pH 8.0, temperature not specified in the publication, wild-type enzyme
0.275
oxaloacetate
-
mutant H320G
111
oxaloacetate
mutant A348G, pH 8.0, temperature not specified in the publication
111
oxaloacetate
pH 8.0, temperature not specified in the publication, mutant enzyme G348G
167
oxaloacetate
-
wild-type
208
oxaloacetate
wild-type, pH 8.0, temperature not specified in the publication
208
oxaloacetate
pH 8.0, temperature not specified in the publication, wild-type enzyme
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19000 - 59000
oxaloacetate
11000
acetyl-CoA
mutant A348G, pH 8.0, temperature not specified in the publication
11000
acetyl-CoA
pH 8.0, temperature not specified in the publication, mutant enzyme G348G
34000
acetyl-CoA
wild-type, pH 8.0, temperature not specified in the publication
34000
acetyl-CoA
pH 8.0, temperature not specified in the publication, wild-type enzyme
19000
oxaloacetate
mutant A348G, pH 8.0, temperature not specified in the publication
19000
oxaloacetate
pH 8.0, temperature not specified in the publication, mutant enzyme G348G
59000
oxaloacetate
wild-type, pH 8.0, temperature not specified in the publication
59000
oxaloacetate
pH 8.0, temperature not specified in the publication, wild-type enzyme
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22.3 - 24.2
3,3-thiodipropionate
22.3 - 24.2
3,3-thiodipropionic acid
21.2 - 71.7
3-phosphonopropionic acid
21.2 - 71.7
3-phosphopropionate
73 - 90.5
Dihydroxyfumarate
73 - 90.5
dihydroxyfumaric acid
8 - 14.6
potassium hydroxycitrate
22.3
3,3-thiodipropionate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
24.2
3,3-thiodipropionate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
22.3
3,3-thiodipropionic acid
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
24.2
3,3-thiodipropionic acid
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
21.2
3-phosphonopropionic acid
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
71.7
3-phosphonopropionic acid
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
21.2
3-phosphopropionate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
71.7
3-phosphopropionate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
73
Dihydroxyfumarate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
90.5
Dihydroxyfumarate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
73
dihydroxyfumaric acid
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
90.5
dihydroxyfumaric acid
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
6.5
DL-malate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
14.2
DL-malate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
6.5
DL-malic acid
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
14.2
DL-malic acid
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
17.5
Maleate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
20
Maleate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
17.5
Maleic acid
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
20
Maleic acid
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
8
potassium hydroxycitrate
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
8
potassium hydroxycitrate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
14.6
potassium hydroxycitrate
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
14.6
potassium hydroxycitrate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
25.1
propionate
pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA
25.1
propionate
substrate acetyl-CoA, pH 8.0, temperature not specified in the publication
28.1
propionate
pH 8.0, temperature not specified in the publication, substrate: oxaloacetate
28.1
propionate
substrate oxaloacetate, pH 8.0, temperature not specified in the publication
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