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Literature summary for 2.3.3.1 extracted from
- Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase (2019), Biol. Chem., 400, 1567-1581 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| comparison of crystal structures of human enzyme and Aspergillus fumigatus 2-methylcitrate synthase, EC 2.3.3.5, both wild-type and mutants A348G and G352A, respectively | Homo sapiens |
| crystallization is performed at room temperature using the sitting-drop vapor diffusion method and MRC 2-drop 96-well crystallization plates | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A348G | mutation to the corresponding residue of Aspergillus fumigatus 2-methylcitrate synthase. Active site residues Arg448 (chain B) and His347 (chain A) are not in their normal positions for oxaloacetate binding | Homo sapiens |
| G386A | insoluble enzyme variant | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3-thiodipropionate | substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive | Homo sapiens |  |
| 3,3-thiodipropionic acid | competitive inhibition versus acetyl-CoA and versus oxaloacetate | Homo sapiens | |
| 3-phosphonopropionic acid | competitive inhibition versus acetyl-CoA and versus oxaloacetate | Homo sapiens | |
| 3-phosphopropionate | substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive | Homo sapiens | |
| Dihydroxyfumarate | substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive | Homo sapiens | |
| dihydroxyfumaric acid | competitive inhibition versus acetyl-CoA and versus oxaloacetate | Homo sapiens | |
| DL-malate | substrate acetyl-CoA, mixed type inhibition; substrate oxaloacetate, mixed type inhibition | Homo sapiens | |
| DL-malic acid | mixed type inhibition versus acetyl-CoA and versus oxaloacetate | Homo sapiens | |
| Maleate | substrate acetyl-CoA, mixed type inhibition; substrate oxaloacetate, mixed type inhibition | Homo sapiens | |
| Maleic acid | mixed type inhibition versus acetyl-CoA and versus oxaloacetate | Homo sapiens | |
| potassium hydroxycitrate | competitive inhibition versus acetyl-CoA and versus oxaloacetate; substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive | Homo sapiens | |
| propionate | competitive inhibition versus oxaloacetate, mixed type inhibition versus acetyl-CoA; substrate acetyl-CoA, competitive; substrate oxaloacetate, competitive | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
oxaloacetate | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0036 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication, wild-type enzyme | Homo sapiens | |
| 0.0057 | - |
oxaloacetate | mutant A348G, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0057 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication, mutant enzyme G348G | Homo sapiens | |
| 0.0077 | - |
acetyl-CoA | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0077 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication, wild-type enzyme | Homo sapiens | |
| 0.0104 | - |
acetyl-CoA | mutant A348G, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0104 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication, mutant enzyme G348G | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O75390 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | - |
Homo sapiens | citrate + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HCS | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 111 | - |
oxaloacetate | mutant A348G, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 111 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication, mutant enzyme G348G | Homo sapiens | |
| 117 | - |
acetyl-CoA | mutant A348G, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 117 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication, mutant enzyme G348G | Homo sapiens | |
| 208 | - |
oxaloacetate | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 208 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication, wild-type enzyme | Homo sapiens | |
| 260 | - |
acetyl-CoA | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 260 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication, wild-type enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.5 | - |
DL-malic acid | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 6.5 | - |
DL-malate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 8 | - |
potassium hydroxycitrate | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 8 | - |
potassium hydroxycitrate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 14.2 | - |
DL-malic acid | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 14.2 | - |
DL-malate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 14.6 | - |
potassium hydroxycitrate | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 14.6 | - |
potassium hydroxycitrate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 17.5 | - |
Maleic acid | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 17.5 | - |
Maleate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 20 | - |
Maleic acid | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 20 | - |
Maleate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 21.2 | - |
3-phosphonopropionic acid | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 21.2 | - |
3-phosphopropionate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 22.3 | - |
3,3-thiodipropionic acid | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 22.3 | - |
3,3-thiodipropionate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 24.2 | - |
3,3-thiodipropionic acid | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 24.2 | - |
3,3-thiodipropionate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 25.1 | - |
propionate | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 25.1 | - |
propionate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 28.1 | - |
propionate | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 28.1 | - |
propionate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 71.7 | - |
3-phosphonopropionic acid | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 71.7 | - |
3-phosphopropionate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 73 | - |
dihydroxyfumaric acid | pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA | Homo sapiens | |
| 73 | - |
Dihydroxyfumarate | substrate acetyl-CoA, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 90.5 | - |
dihydroxyfumaric acid | pH 8.0, temperature not specified in the publication, substrate: oxaloacetate | Homo sapiens | |
| 90.5 | - |
Dihydroxyfumarate | substrate oxaloacetate, pH 8.0, temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11000 | - |
acetyl-CoA | mutant A348G, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 11000 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication, mutant enzyme G348G | Homo sapiens | |
| 19000 | - |
oxaloacetate | mutant A348G, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 19000 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication, mutant enzyme G348G | Homo sapiens | |
| 34000 | - |
acetyl-CoA | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 34000 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication, wild-type enzyme | Homo sapiens | |
| 59000 | - |
oxaloacetate | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 59000 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication, wild-type enzyme | Homo sapiens | |
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