BRENDA - Enzyme Database show
show all sequences of 2.3.3.1

Catalytic strategy of citrate synthase: subunit interactions revealed as a consequence of a single amino acid change in the oxaloacetate binding site

Kurz, L.C.; Shah, S.; Frieden, C.; Nakra, T.; Stein, R.E.; Drysdale, G.R.; Evans, C.T.; Srere, P.A.; Biochemistry 34, 13278-13288 (1995)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
H320G
effect of H320G substitution, solvent accessibility, and conformational changes on catalysis and activity
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
acetyl-CoA
wild-type
Sus scrofa
0.0059
-
oxaloacetate
wild-type
Sus scrofa
0.043
-
oxaloacetate
mutant H320G
Sus scrofa
0.089
-
acetyl-CoA
mutant H320G
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
-
-
Reaction
Reaction
Commentary
Organism
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
active site His320; mechanism
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + oxaloacetate + H2O
intermediate citryl-CoA
488032
Sus scrofa
citrate + CoA
-
488032
Sus scrofa
r
Subunits
Subunits
Commentary
Organism
More
molecular basis of subunit interactions
Sus scrofa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.275
-
oxaloacetate
mutant H320G
Sus scrofa
167
-
oxaloacetate
wild-type
Sus scrofa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H320G
effect of H320G substitution, solvent accessibility, and conformational changes on catalysis and activity
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
acetyl-CoA
wild-type
Sus scrofa
0.0059
-
oxaloacetate
wild-type
Sus scrofa
0.043
-
oxaloacetate
mutant H320G
Sus scrofa
0.089
-
acetyl-CoA
mutant H320G
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + oxaloacetate + H2O
intermediate citryl-CoA
488032
Sus scrofa
citrate + CoA
-
488032
Sus scrofa
r
Subunits (protein specific)
Subunits
Commentary
Organism
More
molecular basis of subunit interactions
Sus scrofa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.275
-
oxaloacetate
mutant H320G
Sus scrofa
167
-
oxaloacetate
wild-type
Sus scrofa
Other publictions for EC 2.3.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735509
Kim
Two pathways for glutamate bio ...
no activity in Syntrophus aciditrophicus
Appl. Environ. Microbiol.
81
8434-8444
2015
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736357
Kim
Identification and characteriz ...
no activity in Syntrophus aciditrophicus
J. Bacteriol.
195
1689-1696
2013
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720857
Crumbley
Regulation of expression of ci ...
Homo sapiens, Mus musculus
PLoS ONE
7
e33804
2012
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1
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4
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1
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2
4
4
2
-
-
720554
Agostinho
Treatment with olanzapine, flu ...
Rattus norvegicus
Neurosci. Lett.
487
278-281
2011
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6
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1
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6
-
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1
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2
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2
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720443
Freitas
Evaluation of citrate synthase ...
Rattus norvegicus
Mol. Cell. Biochem.
341
245-249
2010
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1
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6
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7
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1
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7
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1
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702966
Zanatta
In vitro evidence that D-serin ...
Rattus norvegicus
Brain Res.
1298
186-193
2009
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1
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1
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704788
Cheng
Identification and characteriz ...
Homo sapiens
J. Cell. Biochem.
107
1002-1015
2009
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1
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2
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705092
Weina
Inhibition of citrate synthase ...
Sus scrofa
J. Microbiol. Biotechnol.
19
1628-1634
2009
-
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3
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1
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1
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1
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686061
van der Kamp
High-level QM/MM modelling pre ...
Gallus gallus
Chem. Commun. (Camb. )
2008
1874-1876
2008
-
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1
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689719
Beard
Detailed enzyme kinetics in te ...
Bos taurus, Rattus norvegicus
PLoS ONE
3
e1825
2008
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673500
Ahrman
Small heat shock proteins prev ...
Sus scrofa
Extremophiles
11
659-666
2007
-
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-
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6
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1
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1
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1
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1
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686487
da Silva Pimenta
Effect of a single session of ...
Rattus norvegicus
Eur. J. Appl. Physiol.
102
119-126
2007
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4
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687318
Kohn
Regional specialization of rat ...
Rattus norvegicus
J. Anat.
210
8-18
2007
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2
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3
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3
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689831
Correa
Effects of lithium and valproa ...
Rattus norvegicus
Prog. Neuropsychopharmacol. Biol. Psychiatry
31
887-891
2007
-
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1
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6
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4
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4
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660054
Schlichtholz
Enhanced citrate synthase acti ...
Homo sapiens
Pancreas
30
99-104
2005
-
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667791
Chow
Nardilysin facilitates complex ...
Rattus norvegicus
Biochim. Biophys. Acta
1723
292-301
2005
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4
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674428
Mishra
Efficient refolding of aggrega ...
Sus scrofa
J. Biol. Chem.
280
15553-15560
2005
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1
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1
1
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744711
Kumar
Different roles of electrosta ...
antarctic bacterium DS2-3R, Gallus gallus, Pyrococcus furiosus
ChemBioChem
5
280-290
2004
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5
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3
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2
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3
3
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488083
Leek
Effect of acute exercise on ci ...
Homo sapiens
Am. J. Physiol.
280
R441-447
2001
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1
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3
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1
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2
1
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1
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1
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1
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488073
Ullian
Role of citrate synthase in al ...
Rattus norvegicus
Hypertension
35
875-879
2000
1
-
-
-
-
-
-
-
1
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1
-
3
-
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1
1
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1
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1
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1
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1
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1
1
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1
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1
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-
-
488079
Winger
Characterization of a bovine c ...
Bos taurus
Mol. Reprod. Dev.
55
14-19
2000
-
-
1
-
-
-
-
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1
2
-
4
-
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1
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3
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1
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-
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1
2
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1
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3
-
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-
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-
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-
-
488032
Kurz
Catalytic strategy of citrate ...
Sus scrofa
Biochemistry
34
13278-13288
1995
-
-
-
-
1
-
-
4
-
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-
2
-
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1
-
2
1
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1
1
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2
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1
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4
-
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-
-
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-
2
1
-
1
1
-
-
-
2
-
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-
-
-
-
-
-
-
488052
Takahashi
-
Purification and some properti ...
Nitrobacter winogradskyi
J. Ferment. Bioeng.
77
97-99
1994
-
-
-
-
-
-
6
2
-
1
3
1
-
1
-
-
1
-
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-
1
-
1
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
1
3
1
-
-
-
1
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-
1
-
1
1
1
-
1
-
2
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1
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Isolation, purification and so ...
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1992
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488065
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Regional distribution of citra ...
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1989
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286663
Jeffery
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Citrate synthase and malate de ...
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1988
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