BRENDA - Enzyme Database show
show all sequences of 2.3.3.1

Purification and some properties of citrate synthase from a nitrite-oxidising chemoautotroph, Nitrobacter agilis ATCC 14123

Takahashi, R.; Usui, K.; Sakuraba, T.; Tokuyama, T.; J. Ferment. Bioeng. 77, 97-99 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
no inhibition
Nitrobacter winogradskyi
ATP
no inhibition
Nitrobacter winogradskyi
CuSO4
-
Nitrobacter winogradskyi
HgCl2
-
Nitrobacter winogradskyi
NADH
no effect
Nitrobacter winogradskyi
SDS
strong inhibition
Nitrobacter winogradskyi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
oxaloacetate
-
Nitrobacter winogradskyi
0.41
-
acetyl-CoA
-
Nitrobacter winogradskyi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no activation by metal ions
Nitrobacter winogradskyi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE
Nitrobacter winogradskyi
80000
-
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE
Nitrobacter winogradskyi
250000
-
gel filtration
Nitrobacter winogradskyi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + oxaloacetate + H2O
Nitrobacter winogradskyi
-
citrate + CoA
-
Nitrobacter winogradskyi
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nitrobacter winogradskyi
-
strain ATCC 14123
-
Purification (Commentary)
Commentary
Organism
-
Nitrobacter winogradskyi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
24
-
purified enzyme
Nitrobacter winogradskyi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + oxaloacetate + H2O
-
488052
Nitrobacter winogradskyi
citrate + CoA
-
488052
Nitrobacter winogradskyi
?
Subunits
Subunits
Commentary
Organism
tetramer
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE
Nitrobacter winogradskyi
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
35
-
Nitrobacter winogradskyi
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
-
Nitrobacter winogradskyi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.4
Nitrobacter winogradskyi
7.5
8
-
Nitrobacter winogradskyi
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
-
Nitrobacter winogradskyi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
no inhibition
Nitrobacter winogradskyi
ATP
no inhibition
Nitrobacter winogradskyi
CuSO4
-
Nitrobacter winogradskyi
HgCl2
-
Nitrobacter winogradskyi
NADH
no effect
Nitrobacter winogradskyi
SDS
strong inhibition
Nitrobacter winogradskyi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
oxaloacetate
-
Nitrobacter winogradskyi
0.41
-
acetyl-CoA
-
Nitrobacter winogradskyi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no activation by metal ions
Nitrobacter winogradskyi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE
Nitrobacter winogradskyi
80000
-
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE
Nitrobacter winogradskyi
250000
-
gel filtration
Nitrobacter winogradskyi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + oxaloacetate + H2O
Nitrobacter winogradskyi
-
citrate + CoA
-
Nitrobacter winogradskyi
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nitrobacter winogradskyi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
24
-
purified enzyme
Nitrobacter winogradskyi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + oxaloacetate + H2O
-
488052
Nitrobacter winogradskyi
citrate + CoA
-
488052
Nitrobacter winogradskyi
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE
Nitrobacter winogradskyi
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
35
-
Nitrobacter winogradskyi
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
-
Nitrobacter winogradskyi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.4
Nitrobacter winogradskyi
7.5
8
-
Nitrobacter winogradskyi
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
-
Nitrobacter winogradskyi
Other publictions for EC 2.3.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735509
Kim
Two pathways for glutamate bio ...
no activity in Syntrophus aciditrophicus
Appl. Environ. Microbiol.
81
8434-8444
2015
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736357
Kim
Identification and characteriz ...
no activity in Syntrophus aciditrophicus
J. Bacteriol.
195
1689-1696
2013
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720857
Crumbley
Regulation of expression of ci ...
Homo sapiens, Mus musculus
PLoS ONE
7
e33804
2012
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1
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2
4
4
2
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720554
Agostinho
Treatment with olanzapine, flu ...
Rattus norvegicus
Neurosci. Lett.
487
278-281
2011
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720443
Freitas
Evaluation of citrate synthase ...
Rattus norvegicus
Mol. Cell. Biochem.
341
245-249
2010
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1
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1
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702966
Zanatta
In vitro evidence that D-serin ...
Rattus norvegicus
Brain Res.
1298
186-193
2009
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704788
Cheng
Identification and characteriz ...
Homo sapiens
J. Cell. Biochem.
107
1002-1015
2009
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705092
Weina
Inhibition of citrate synthase ...
Sus scrofa
J. Microbiol. Biotechnol.
19
1628-1634
2009
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1
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686061
van der Kamp
High-level QM/MM modelling pre ...
Gallus gallus
Chem. Commun. (Camb. )
2008
1874-1876
2008
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689719
Beard
Detailed enzyme kinetics in te ...
Bos taurus, Rattus norvegicus
PLoS ONE
3
e1825
2008
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673500
Ahrman
Small heat shock proteins prev ...
Sus scrofa
Extremophiles
11
659-666
2007
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6
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686487
da Silva Pimenta
Effect of a single session of ...
Rattus norvegicus
Eur. J. Appl. Physiol.
102
119-126
2007
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687318
Kohn
Regional specialization of rat ...
Rattus norvegicus
J. Anat.
210
8-18
2007
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689831
Correa
Effects of lithium and valproa ...
Rattus norvegicus
Prog. Neuropsychopharmacol. Biol. Psychiatry
31
887-891
2007
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6
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660054
Schlichtholz
Enhanced citrate synthase acti ...
Homo sapiens
Pancreas
30
99-104
2005
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667791
Chow
Nardilysin facilitates complex ...
Rattus norvegicus
Biochim. Biophys. Acta
1723
292-301
2005
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674428
Mishra
Efficient refolding of aggrega ...
Sus scrofa
J. Biol. Chem.
280
15553-15560
2005
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744711
Kumar
Different roles of electrosta ...
antarctic bacterium DS2-3R, Gallus gallus, Pyrococcus furiosus
ChemBioChem
5
280-290
2004
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5
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3
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2
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3
3
-
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-
488083
Leek
Effect of acute exercise on ci ...
Homo sapiens
Am. J. Physiol.
280
R441-447
2001
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1
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1
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1
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488073
Ullian
Role of citrate synthase in al ...
Rattus norvegicus
Hypertension
35
875-879
2000
1
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1
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1
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3
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1
1
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1
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1
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1
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1
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488079
Winger
Characterization of a bovine c ...
Bos taurus
Mol. Reprod. Dev.
55
14-19
2000
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1
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2
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4
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488032
Kurz
Catalytic strategy of citrate ...
Sus scrofa
Biochemistry
34
13278-13288
1995
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1
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4
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2
1
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1
1
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2
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488052
Takahashi
-
Purification and some properti ...
Nitrobacter winogradskyi
J. Ferment. Bioeng.
77
97-99
1994
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6
2
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1
3
1
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6
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2
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1
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1
1
1
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2
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1
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488055
Morgunov
-
Isolation, purification and so ...
Yarrowia lipolytica
Biokhimiya
59
1320-1329
1994
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2
4
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2
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1
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1
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1
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488058
Kelly
Reactivation of denatured citr ...
Sus scrofa
Int. J. Biochem.
24
627-630
1992
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2
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1
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488065
Sylven
Regional distribution of citra ...
Bos taurus
Acta Physiol. Scand.
136
331-337
1989
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1
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286663
Jeffery
-
Citrate synthase and malate de ...
Solanum lycopersicum
Phytochemistry
27
41-44
1988
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2
2
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2
1
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1
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