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Literature summary for 2.3.3.1 extracted from
- Purification and some properties of citrate synthase from a nitrite-oxidising chemoautotroph, Nitrobacter agilis ATCC 14123 (1994), J. Ferment. Bioeng., 77, 97-99.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | no inhibition | Nitrobacter winogradskyi |  |
| ATP | no inhibition | Nitrobacter winogradskyi | |
| CuSO4 | - |
Nitrobacter winogradskyi | |
| HgCl2 | - |
Nitrobacter winogradskyi | |
| NADH | no effect | Nitrobacter winogradskyi | |
| SDS | strong inhibition | Nitrobacter winogradskyi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
oxaloacetate | - |
Nitrobacter winogradskyi | |
| 0.41 | - |
acetyl-CoA | - |
Nitrobacter winogradskyi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by metal ions | Nitrobacter winogradskyi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE | Nitrobacter winogradskyi |
| 80000 | - |
2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE | Nitrobacter winogradskyi |
| 250000 | - |
gel filtration | Nitrobacter winogradskyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | Nitrobacter winogradskyi | - |
citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nitrobacter winogradskyi | - |
strain ATCC 14123 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nitrobacter winogradskyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
purified enzyme | Nitrobacter winogradskyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Nitrobacter winogradskyi | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE | Nitrobacter winogradskyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Nitrobacter winogradskyi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Nitrobacter winogradskyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.4 | Nitrobacter winogradskyi |
| 7.5 | 8 | - |
Nitrobacter winogradskyi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Nitrobacter winogradskyi |
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