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EC Number Renatured (Commentary) Reference
Show all pathways known for 2.3.3.1Display the word mapDisplay the reaction diagram Show all sequences 2.3.3.1only after removal of 6 M denaturing guanidinium chloride by dialysis, not by dilution, dithiothreitol-dependent 488058
Show all pathways known for 2.3.3.1Display the word mapDisplay the reaction diagram Show all sequences 2.3.3.1study on the mechanism of aggregation during refolding of enzyme and its prevention using cosolvent additives of the polyol series. No parallel correlation between the folding effect and the general stabilization is observed. Glycerol is most effective in enhancing the refolding yield of citrate synthase, and a complete recovery of enzymatic activity is observed at 7 M glycerol and 0.01 mg per ml protein. Kinetic experiments suggest that polyols act very early in the refolding process. Both the thermodynamic and the kinetic aspects are critical in the folding process 674428
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