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Information on EC 2.3.2.24 - (E3-independent) E2 ubiquitin-conjugating enzyme

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme
IUBMB Comments
The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC 2.3.2.26, EC 2.3.2.27). It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg2+-ATP and catalyses the conjugation of ubiquitin to protein substrates, independently of E3. This transfer has only been observed with small proteins. In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L-lysine methyl ester) has been observed .
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
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[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E1 ubiquitin-activating enzyme]-L-cysteine
+
[acceptor protein]-N6-monoubiquitinyl-L-lysine
Synonyms
arsenite-sensitive E2, biquitin carrier protein D4, biquitin-conjugating enzyme E2 T, CDC34, CLF_100482, E2 ubiquitin-conjugating enzyme, E2230K, HIP2, N-terminus-conjugating E2, UB carrier protein (E2), more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine
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(1b)
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-
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[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine
show the reaction diagram
(1a)
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[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine
show the reaction diagram
PATHWAY SOURCE
PATHWAYS
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