EC Number   |
General Information |
Reference |
|---|
   2.3.2.24 | evolution |
humans have about 40 E2s that are involved in the transfer of Ub or Ub-like (Ubl) proteins (e.g. SUMO and NEDD8). Common functional and structural features that define unifying themes among E2s, overview. Highly specific chain builders such as Ube2N, Ube2S, and Ube2R1 can only transfer their conjugated Ub to another Ub molecule. This leads to a division of labor among E2s in which one E2 initiates or primes chain synthesis and a second E2 builds and extends the polyUb chain |
759030 |
| 2.3.2.24 | evolution |
ubiquitin-conjugating enzyme E2 O (UBE2O) is a member of the E2 family of the ubiquitin-proteasome system (UPS). Unlike most members of this family which are about 20 to 25 kDa in molecular weight, UBE2O is unusually large, with a molecular weight of 141 kDa |
759177 |
| 2.3.2.24 | malfunction |
a UCP mutant in which Cys118 is changed to alanine (UCPC118A) does not form a polyubiquitin chain but strongly accumulates mono- and di-ubiquitin via auto-ubiquitination |
760018 |
| 2.3.2.24 | malfunction |
inhibition of UBE2O or depletion of UBE2O leads to the reduced growth of breast and prostate cancer cells, but inactivation of AMPKalpha2 abrogates the beneficial effect caused by the UBE2O loss |
759177 |
| 2.3.2.24 | malfunction |
UBE2O knockdown elevates the amplitude of the circadian clock in human osteosarcoma U2OS cells. Full-length UBE2O and the CR2 truncation rescue the effect of UBE2O knockdown on the BMAL1 protein level |
759488 |
| 2.3.2.24 | metabolism |
the ubiquitin-proteasome system is an important regulatory machinery involved in proteostasis and cellular signaling. Proteins are ubiquitinated via the concerted action of E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin ligases. UBE2O is an E2/E3 hybrid enzyme and exhibits both the E2 and E3 activities. Role of UBE2O in the development of hematological disorders and cancers, overview |
759177 |
| 2.3.2.24 | metabolism |
UBE2O is a critical regulator in the ubiquitin-proteasome system, which modulates BMAL1 transcriptional activity and circadian function by promoting BMAL1 ubiquitination and degradation under normal physiological conditions. UBE2O regulates BMAL1 protein level and stability |
759488 |
| 2.3.2.24 | metabolism |
ubiquitin-conjugating enzymes (E2s) are the central players in the trio of enzymes responsible for the attachment of ubiquitin (Ub) to cellular proteins. E2 regulation mechanisms, overview |
759030 |
| 2.3.2.24 | more |
E2 structure-function analysis, overview. Enzyme UBE2O is a large multidomain E2/E3 hybrid performing the E2 and the E3 reaction |
759030 |
| 2.3.2.24 | more |
homology modeling of UBE2O, using the recombinant enzyme and structure of the MZM-REP domains of Mind bomb 1 (Mib1, PDB ID 4XI6) and the UBC domain of baculoviral IAP repeat-containing protein 6 (BIRC6, PDB ID 3CEG), respectively, overview. UBE2O also displays nonenzymatic functions, and as a protein-interacting partner it can block protein ubiquitination. The N-terminus of UBE2O interacts efficiently with the TRAF domain in TNF receptor-associated factor 6 (TRAF6), prevents its K63-linked polyubiquitination, competes with myeloid differentiation primary response protein MyD88 for TRAF6, and thus suppresses the activation of NF-kappaB induced by lipopolysaccharide and interleukin-1beta. Loss of the UBC domain in UBE2O does not affect this regulation, indicating that its nonenzymatic function prevents TRAF6 ubiquitination |
759177 |
