EC Number |
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2.3.2.24 | expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag |
2.3.2.24 | expression in Escherichia coli |
2.3.2.24 | gene UBE2O, cloned from liver, DNA and amino acid sequence determination and comparisons |
2.3.2.24 | gene UBE2O, recombinant expression of Strep-tagged UBE2O in HEK-293 cells, coexpression with FLAG-tagged BMAL1 transcription factor |
2.3.2.24 | recombinant expression of diversely tagged wild-type and mutant UCP enzymes in HEK-293T cells, the activity of the recombinant mutant enzymes varies dependent on the fusion tag, overview |