Literature summary for 2.3.2.24 extracted from

David, Y.; Ziv, T.; Admon, A.; Navon, A.
The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines (2010), J. Biol. Chem., 285, 8595-8604.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	O00762	-	-
Homo sapiens	P49427	-	-
Homo sapiens	P49459	-	-
Homo sapiens	P51668	-	-
Homo sapiens	P51965	-	-
Homo sapiens	P60604	-	-
Homo sapiens	P61077	-	-
Homo sapiens	P61086	-	-
Homo sapiens	P61088	-	-
Homo sapiens	P62253	-	-
Homo sapiens	P62256	-	-
Homo sapiens	P62837	-	-
Homo sapiens	P63146	-	-
Homo sapiens	P68036	-	-
Homo sapiens	Q13404	-	-
Homo sapiens	Q15819	-	-
Homo sapiens	Q16763	-	-
Homo sapiens	Q712K3	-	-
Homo sapiens	Q8WVN8	-	-
Homo sapiens	Q969T4	-	-
Homo sapiens	Q96B02	-	-
Homo sapiens	Q96LR5	-	-
Homo sapiens	Q9NPD8	-	-
Homo sapiens	Q9Y2X8	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, Ni-NTA column	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11, as well as Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin, with Lys11 as common as those of Lys48. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical polyubiquitin chains that are linked through Lys63	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11-and Lys48-, as well as Lys63-linked polyubiquitination.	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes Lys11-linked polyubiquitination. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer Lys11-, Lys27-, Lys48-, and Lys-63-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. In vitro catalyzes Lys11-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11- and Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11-, as well as Lys48- and Lys63-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of Lys48-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin, with Lys11 as common as those of Lys48	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin, with Lys11 as common as those of Lys48. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11- and Lys48-, as well as Lys63-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitinAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys48-linked polyubiquitination	Homo sapiens	a
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys63-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	polyubiquitin chain formation catalyzed by E2 enzymes, in the absence of an E3 protein and a target protein substrate	Homo sapiens	?	a thiol ester-linked ubiquitin to the E2 active site is an intermediate in any polyubiquination reactions	?

Subunits

Subunits	Comment	Organism
dimer	E2 enzymes spontaneeously dimerize in solution, in vitro, in absence of charged ubiquitin	Homo sapiens
dimer	E2 enzymes spontaneously dimerize in solution, in vitro, in absence of charged ubiquitin	Homo sapiens

Synonyms

Synonyms	Comment	Organism
biquitin carrier protein D4	-	Homo sapiens
biquitin-conjugating enzyme E2 T	-	Homo sapiens
E2 ubiquitin-conjugating enzyme	-	Homo sapiens
N-terminus-conjugating E2	-	Homo sapiens
UBE2A	-	Homo sapiens
UBE2B	-	Homo sapiens
UBE2C	-	Homo sapiens
UBE2D1	-	Homo sapiens
UBE2D2	-	Homo sapiens
UBE2D3	-	Homo sapiens
UBE2D4	-	Homo sapiens
UBE2E1	-	Homo sapiens
UBE2E2	-	Homo sapiens
UBE2E3	-	Homo sapiens
UBE2G1	-	Homo sapiens
UBE2G2	-	Homo sapiens
UBE2H	-	Homo sapiens
UBE2K	-	Homo sapiens
UBE2L3	-	Homo sapiens
UBE2N	-	Homo sapiens
UBE2Q2	-	Homo sapiens
UBE2R1	-	Homo sapiens
UBE2R2	-	Homo sapiens
UBE2S	-	Homo sapiens
UBE2T	-	Homo sapiens
UBE2V1	-	Homo sapiens
UBE2V2	-	Homo sapiens
UBE2W	-	Homo sapiens
ubiquitin carrier protein A	-	Homo sapiens
ubiquitin carrier protein B	-	Homo sapiens
ubiquitin carrier protein C	-	Homo sapiens
ubiquitin carrier protein D1	-	Homo sapiens
ubiquitin carrier protein D2	-	Homo sapiens
ubiquitin carrier protein D3	-	Homo sapiens
Ubiquitin carrier protein E1	-	Homo sapiens
ubiquitin carrier protein E2	-	Homo sapiens
ubiquitin carrier protein E3	-	Homo sapiens
Ubiquitin carrier protein G1	-	Homo sapiens
Ubiquitin carrier protein G2	-	Homo sapiens
ubiquitin carrier protein H	-	Homo sapiens
Ubiquitin carrier protein L3	-	Homo sapiens
ubiquitin carrier protein N	-	Homo sapiens
Ubiquitin carrier protein Q2	-	Homo sapiens
ubiquitin carrier protein R2	-	Homo sapiens
ubiquitin carrier protein S	-	Homo sapiens
ubiquitin carrier protein T	-	Homo sapiens
ubiquitin carrier protein W	-	Homo sapiens
ubiquitin-conjugating enzyme E2 A	-	Homo sapiens
ubiquitin-conjugating enzyme E2 B	-	Homo sapiens
ubiquitin-conjugating enzyme E2 C	-	Homo sapiens
ubiquitin-conjugating enzyme E2 D1	-	Homo sapiens
ubiquitin-conjugating enzyme E2 D2	-	Homo sapiens
ubiquitin-conjugating enzyme E2 D3	-	Homo sapiens
ubiquitin-conjugating enzyme E2 D4	-	Homo sapiens
ubiquitin-conjugating enzyme E2 E1	-	Homo sapiens
ubiquitin-conjugating enzyme E2 E2	-	Homo sapiens
ubiquitin-conjugating enzyme E2 E3	-	Homo sapiens
ubiquitin-conjugating enzyme E2 G1	-	Homo sapiens
ubiquitin-conjugating enzyme E2 G2	-	Homo sapiens
ubiquitin-conjugating enzyme E2 H	-	Homo sapiens
ubiquitin-conjugating enzyme E2 K	-	Homo sapiens
ubiquitin-conjugating enzyme E2 L3	-	Homo sapiens
ubiquitin-conjugating enzyme E2 N	-	Homo sapiens
Ubiquitin-conjugating enzyme E2 Q2	-	Homo sapiens
ubiquitin-conjugating enzyme E2 R1	-	Homo sapiens
ubiquitin-conjugating enzyme E2 R2	-	Homo sapiens
ubiquitin-conjugating enzyme E2 S	-	Homo sapiens
ubiquitin-conjugating enzyme E2 variant 1	-	Homo sapiens
ubiquitin-conjugating enzyme E2 variant 2	-	Homo sapiens
ubiquitin-conjugating enzyme E2 W	-	Homo sapiens
ubiquitin-conjugating enzyme E2-25 kDa	-	Homo sapiens
ubiquitin-conjugating enzyme E2-CDC34	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at, polyubiquitination	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at, polyubiquitination	Homo sapiens