EC Number   |
Protein Variants |
Reference |
|---|
   2.3.2.24 | C118A |
site-directed mutagenesis, inactive mutant |
760018 |
| 2.3.2.24 | C95A |
site-directed mutagenesis, the mutant shows impaired ubiquination activity |
760018 |
| 2.3.2.24 | K76R/K100R |
site-directed mutagenesis, the mutant shows reduced autoubiquitination activity compared to wild-type |
760018 |
| 2.3.2.24 | more |
construction of UCP knockdown cells using a UCP gene-specific shRNA construct. Although UCPDELTAN deletion mutant harbors catalytic active cysteine residues, it cannot ubiquitinate Hippel-Lindau protein (pVHL) due to the missing N-terminus, which forms part of the binding site for pVHL. The UCPC95A/UCPDELTAN pair degrades pVHL in vivo, whereas the UCPDELTAN/UCPC118A pair cannot degrade pVHL. The activity of the recombinant mutant enzymes varies dependent on the fusion tag, overview |
760018 |
| 2.3.2.24 | more |
UBE2O knockdown in U2-O2 cells. UBE2O knockdown elevates the amplitude of the circadian clock in human osteosarcoma U2OS cells. Full-length UBE2O and the CR2 truncation rescue the effect of UBE2O knockdown on the BMAL1 protein level |
759488 |
