Information on EC 1.14.17.3 - peptidylglycine monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.17.3
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RECOMMENDED NAME
GeneOntology No.
peptidylglycine monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
peptidylglycine + ascorbate + O2 = peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-H bond cleavage
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C-N bond cleavage
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dealkylation
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hydroxylation
O-dealkylation
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O-oxidative dealkylation of benzaldehyde imino-oxy acetic acid
oxidation
redox reaction
reduction
SYSTEMATIC NAME
IUBMB Comments
peptidylglycine,ascorbate:oxygen oxidoreductase (2-hydroxylating)
A copper protein. Peptidylglycines with a neutral amino acid residue in the penultimate position are the best substrates for the enzyme. The product is unstable and dismutates to glyoxylate and the corresponding desglycine peptide amide, a reaction catalysed by EC 4.3.2.5 peptidylamidoglycolate lyase. Involved in the final step of biosynthesis of alpha-melanotropin and related biologically active peptides.
CAS REGISTRY NUMBER
COMMENTARY hide
90597-47-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
2 forms: a soluble and a membrane-bound; marine mollusc
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Q4W7B5
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Frog
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
copper metabolism is altered by PAM
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(([(1E)-phenylmethylidene]amino)oxy)acetic acid + ascorbate + O2
(2R)-hydroxy(([(1E)-phenylmethylidene]amino)oxy)ethanoic acid + ?
show the reaction diagram
-
non-enzymatic dealkylation yields benzaldoxime and glyoxylate
-
-
?
2,6-difluorohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
2-aminohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
2-hydroxyhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
2-iodohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
2-methylhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
2-propylmercaptoacetylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
2-pyridylmercaptoacetylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
3-(2-furyl)acryloylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
3-chlorohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
3-indolylacetylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
3-methylhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
3-phenylthiopropionylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-aminohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-bromohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-chlorohippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + ascorbate + O2
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
4-ethylhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-hydroxyhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-methoxyhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-methylhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-nitrobenzoyl-Gly-Gly + ascorbate + O2
?
show the reaction diagram
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-
-
-
?
4-nitrohippuric acid + ascorbate + O2
?
show the reaction diagram
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-
-
-
?
4-phenylbutyrylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
4-propylhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
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?
4-trifluoromethylhippuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
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?
5-phenylpentanoylglycine + ascorbate + O2
?
show the reaction diagram
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-
-
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?
6-phenylhexanoylglycine + ascorbate + O2
?
show the reaction diagram
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-
-
-
?
8-phenyloctanoylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
Ac-Tyr-Val-Gly + ascorbate + O2
Ac-Tyr-Val-(2-OH-Gly) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
acetyl-D-alanine + ascorbate + O2
?
show the reaction diagram
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-
-
-
?
acetyl-Gly-Gly + ascorbate + O2
?
show the reaction diagram
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-
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?
acetyl-L-Tyr-L-Val-Gly + ascorbate + O2
?
show the reaction diagram
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-
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?
adrenocorticotrophic hormone(9-14) + ascorbate + O2
adrenocorticotrophic hormone(9-13)-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
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-
-
?
Ala-Ile-Gly-Val-Gly-Ala-Pro-Gly + ascorbate + O2
Ala-Ile-Gly-Val-Gly-Ala-Pro-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
alanyl-L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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-
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?
alanyl-L-prolylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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?
alanylglycyl-L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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?
alanylglycyl-L-prolylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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?
alanylglycylvalyl-L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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?
alanylglycylvalyl-L-prolylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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-
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?
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2
alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2
alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
benzaldehyde imino-oxy acetic acid + O2
benzaldoxime + glyoxylate
show the reaction diagram
-
assay at 37C, pH 6.0
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?
benzoyl-D-alanine + ascorbate + O2
?
show the reaction diagram
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?
calcitonin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
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?
CBZ-D-alanine + ascorbate + O2
?
show the reaction diagram
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?
CBZ-glycine + ascorbate + O2
?
show the reaction diagram
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?
chloroacetyl-Gly-Gly + ascorbate + O2
?
show the reaction diagram
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?
chromogranin A + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
cinnamoylglycine + ascorbate + O2
?
show the reaction diagram
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-
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?
D-iodo-Tyr-Val-Gly + ascorbate + O2
D-iodo-Tyr-Val-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
labeled substrate
the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide
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?
D-Tyr-Pro-Gly-Gly + ascorbate + O2
D-Tyr-Pro-Gly-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
D-Tyr-Val-Gly + ascorbate + O2
D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
D-Tyr-Val-Gly + ascorbate + O2
D-Tyr-Val-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
dansyl-D-Tyr-Val-Gly + ascorbate + O2
dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
dansyl-D-Tyr-Val-Gly + H2O2
dansyl-D-Tyr-Val-2-hydroxyglycine + H2O
show the reaction diagram
-
peptidylglycine monooxygenase is able to catalyze the hydroxylation of peptidylglycine substrates starting from the oxidized enzyme and using hydrogen peroxide as the only source of oxygen
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-
?
dansyl-Gly-Gly-Ser-CO-NH-CH2-COOH + ascorbate + O2
?
show the reaction diagram
-
substrate contains either protium or deuterium at the CH2-group
-
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?
dansyl-L-Tyr-L-Val-Gly + ascorbate + O2
?
show the reaction diagram
dansyl-YVG + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
dansyl-YVG-COOH + ascorbate + O2
dansyl-YV-NH2 + dehydroascorbate + H2O
show the reaction diagram
diiodotyrosylglycine + ascorbate + O2
diiodotyrosyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
gamma-Glu-Gly-Gly + ascorbate + O2
gamma-Glu-Gly-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
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-
-
-
?
glutathione + ascorbate + O2
gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
glyoxylic acid phenylhydrazone + ascorbate + O2
oxalate phenylhydrazide + dehydroascorbate + H2O
show the reaction diagram
-
competitive substrate, inhibitory
-
?
hippuric acid + ascorbate + O2
?
show the reaction diagram
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-
-
-
?
hippuric acid + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
hippuric acid + ascorbate + O2
alpha-hydroxyhippuric acid + dehydroascorbate + H2O
show the reaction diagram
hippuric acid + ascorbate + O2
glyoxylate + dehydroascorbate + H2O
show the reaction diagram
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-
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-
?
hippuric acid + ferrocyanide + O2
alpha-hydroxyhippuric acid + ? + H2O
show the reaction diagram
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-
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hippuryl-Gly + ascorbate + O2
?
show the reaction diagram
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-
-
-
?
hippuryl-Gly-Gly + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
HOOC-NH-CH2-COOH + ascorbate + O2
?
show the reaction diagram
-
substrate contains either protium or deuterium at the CH2-group
-
-
?
hydrocinnamoylglycine + ascorbate + O2
?
show the reaction diagram
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-
-
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?
insulin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
insulin glargine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
isocaproylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
L-prolylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
L-pyroglutamyl-Gly + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
leukotriene C4 + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
monoiodo-alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2
monoiodo-alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
monoiodo-D-Tyr-Val-Gly + ascorbate + O2
monoiodo-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
synthetic peptid
-
?
N-(2,4,6-trinitrophenyl)-D-Tyr-Val-Gly + ascorbate + O2
N-(2,4,6-trinitrophenyl)-D-Tyr-Val-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-(2-furoyl)glycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-(2-thienylcarbonyl)glycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-(4-amino-6-methyl-3-oxoheptanoyl)-L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-(4-amino-6-methyl-3-oxoheptanoyl)glycylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-(4-nitrobenzyl)glycine + ascorbate + O2
nitrobenzylamine + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
N-(alpha-methylhydrocinnamoyl)glycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-(phenylacetyl)glycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-(phenylacetyl)glycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetyl-Arg-Gly-Gly + ascorbate + O2
N-acetyl-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
-
N-acetyl-Gln-Lys-Glu-Ser-Thr-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly + ascorbate + O2
N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
-
N-acetyl-L-Phe-Gly + ascorbate + O2
N-acetyl-L-Phe-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetyl-L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetyl-Leu-Arg-Gly-Gly + ascorbate + O2
N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
-
N-acetyl-Leu-Arg-Leu-Arg-Gly-Gly + ascorbate + O2
N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
show the reaction diagram
-
-
-
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-
N-acetyl-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly + ascorbate + O2
N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
show the reaction diagram
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-
-
-
-
N-acetyl-Tyr-Val-Gly + ascorbate + O2
N-acetyl-Tyr-Val-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetylglycine + 2 ascorbate + O2
N-acetyl-2-hydroxyglycine + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-acetylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetylglycine + ascorbate + O2
acetylamine + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetylglycine + ascorbate + O2
N-acetyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-acetylglycylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-benzoylglycine + ascorbate + O2
N-benzoyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-butyrylglycine + 2 ascorbate + O2
N-butyryl-2-hydroxyglycine + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-Dan-Tyr-Val-Gly + ascorbate + O2
N-Dan-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
N-DANSYL-D-Tyr-L-Val-Gly + ascorbate + O2
N-DANSYL-D-Tyr-L-Val-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-dansyl-Tyr-Val-D-Ala + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-dansyl-Tyr-Val-Gly + ascorbate + O2
N-dansyl-Tyr-Val-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
N-decanoylglycine + 2 ascorbate + O2
N-decanoyl-2-hydroxyglycine + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-decanoylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-decanoylglycine + ascorbate + O2
decanoylamine + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-hexanoylglycine + 2 ascorbate + O2
N-hexanoyl-2-hydroxyglycine + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-hexanoylglycine + ascorbate + O2
hexanoylamine + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-octanoylglycine + 2 ascorbate + O2
N-octanoyl-2-hydroxyglycine + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-propionylglycine + 2 ascorbate + O2
N-propionyl-2-hydroxyglycine + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-trifluoroacetylglycine + ascorbate + O2
N-trifluoroacetyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2
N-trinitrophenyl-D-Tyr-Val-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
N-trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2
N-trinitrophenyl-D-Tyr-Val-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
Frog
-
synthetic substrate
-
-
?
N-[(benzylmercapto)carbonyl]glycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-[(phenylcyclopropyl)carbonyl]glycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
N-[3-(1-leucylpyrrolidin-2-yl)-3-oxopropanoyl]-L-phenylalanylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
N-[3-(1-leucylpyrrolidin-2-yl)-3-oxopropanoyl]glycylglycine + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
nicotinuric acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
O-acetyl-L-mandelyl-Gly + ascorbate + O2
O-acetyl-L-mandelyl-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
oxytoxin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
peptidyl-glycine + ascorbate + O2
peptidyl-(2-hydroxylglycine) + dehydroascorbate + H2O
show the reaction diagram
peptidylglycine + 2 ascorbate + O2
peptidyl(2-hydroxyglycine) + 2 semidehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
peptidylglycine + ascorbate + O2
peptidyl (S)-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
peptidylglycine + ascorbate + O2
peptidyl alpha-hydroxyglycine + semidehydroascorbate + H2O
show the reaction diagram
peptidylglycine + ascorbate + O2
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
peptidylglycine + ascorbate + O2
peptidyl(2-hydroxylglycine) + dehydroascorbate + H2O
show the reaction diagram
-
peptidylglycine alpha-hydroxylating monooxygenase reaction
the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide
-
?
Phe-Gly-Phe-Gly + ascorbate + O2
Phe-Gly-Phe-(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
Phe-Gly-Phe-Gly + ascorbate + O2
Phe-Gly-Phe-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
phenylhydantoic acid + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
phenylmercaptoacetylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
phenylthioacetylglycine + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
proopiomelanocortin peptide + ascorbate + O2
proopiomelanocortin peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
R-CO-NH-CH2-COOH + ascorbate + O2
R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
show the reaction diagram
S-(1,2-dicarboxyethyl)-glutathione + ascorbate + O2
S-(1,2-dicarboxyethyl)-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-(4-nitrobenzyl)-glutathione + ascorbate + O2
S-(4-nitrobenzyl)-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-butyl-glutathione + ascorbate + O2
S-butyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-decyl-glutathione + ascorbate + O2
S-decyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-ethyl-glutathione + ascorbate + O2
S-ethyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-hexyl-glutathione + ascorbate + O2
S-hexyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-methyl-glutathione + ascorbate + O2
S-methyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-octyl-glutathione + ascorbate + O2
S-octyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
S-propyl-glutathione + ascorbate + O2
S-propyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
substance P + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
tBOC-Gly + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
tBOC-Gly-Gly + ascorbate + O2
?
show the reaction diagram
-
-
-
-
?
thyrotropin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2
?
show the reaction diagram
-
activity assay
-
-
?
trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2
trinitrophenyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
show the reaction diagram
-
COOH-terminal glycine
-
-
?
ubiquitin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
-
[(4-nitrobenzyl)oxy]acetic acid + ascorbate + O2
nitrobenzyl alcohol + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
calcitonin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
glutathione + ascorbate + O2
gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
insulin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
oxytoxin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
peptidyl-glycine + ascorbate + O2
peptidyl-(2-hydroxylglycine) + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
peptidylglycine + ascorbate + O2
peptidyl alpha-hydroxyglycine + semidehydroascorbate + H2O
show the reaction diagram
peptidylglycine + ascorbate + O2
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
peptidylglycine + ascorbate + O2
peptidyl(2-hydroxylglycine) + dehydroascorbate + H2O
show the reaction diagram
-
peptidylglycine alpha-hydroxylating monooxygenase reaction
the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide
-
?
proopiomelanocortin peptide + ascorbate + O2
proopiomelanocortin peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
show the reaction diagram
R-CO-NH-CH2-COOH + ascorbate + O2
R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
substance P + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
thyrotropin + ascorbate + O2
? + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
FAD
-
slight activity with
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
3.26 mol per mol of bifunctional enzyme
Fe2+
0.37 mol per mol of bifunctional enzyme
Mg2+
0.88 mol per mol of bifunctional enzyme
additional information
-
Ag(I) is iso-electronic with Cu(I) and has been shown to be handled by similar cellular transport processes. Although complexes of Ag in the Ag(III) state exists, the instability of the Ag(II) state renders the metal unsuitable for a functional substitute for copper in enzymes such as PHM. When enzyme mutants H107A/H108A and M109I (a wild-type analogue with both copper sites intact) are incubated with excess AgNO3, each variant binds a single Ag(I) ion, from which it is inferred that Ag(I) binds selectively at the M-center with little or no affinity for the H-center
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2E)-4-oxo-4-phenylbut-2-enoic acid
-
-
(3E)-4-(4-methoxyphenyl)but-3-enoic acid
-
-
(3E)-4-(4-methylphenyl)but-3-enoic acid
-
-
(3E)-4-[3-(benzyloxy)-4-methoxyphenyl]but-3-enoic acid
-
-
(3E)-4-[4-(benzyloxy)phenyl]but-3-enoic acid
-
-
(acetyloxy)acetic acid
-
-
(CBZ-hydrazido)glycine
-
-
(decanoylsulfanyl)acetic acid
-
-
(dodecanoyloxy)acetic acid
-
-
(E)-(4-dimethylamino)cinnamic acid
-
-
-
(E)-3,4-methylenedioxycinnamic acid
-
-
-
(E)-4-aminocinnamic acid
-
-
-
(nicotinamidomethyl)phosphonic acid
-
-
(R)-5-acetamido-4-oxo-6-phenyl-2-hexenoic acid
-
reversible and irreversible inhibition
(S)-5-acetamido-4-oxo-6-phenyl-2-hexenoic acid
-
reversible and irreversible inhibition
(S)-5-acetamido-7-methylthio-4-oxo-2-heptenoic acid
-
reversible and irreversible inhibition
(S)-N-CBZ-4-amino-2-hydroxybutyric acid
-
-
([(2S)-2-[(2-aminopropanoyl)amino]-3-phenylpropanoyl]oxy)acetic acid
-
-
([N-[3-(1-leucylpyrrolidin-2-yl)-3-oxopropanoyl]-L-phenylalanyl]oxy)acetic acid
-
-
([N-[3-(1-leucylpyrrolidin-2-yl)-3-oxopropanoyl]glycyl]oxy)acetic acid
-
-
([[(2S)-1-(2-aminopropanoyl)pyrrolidin-2-yl]carbonyl]oxy)acetic acid
-
-
([[(2S)-1-[[(2-aminopropanoyl)amino]acetyl]pyrrolidin-2-yl]carbonyl]oxy)acetic acid
-
-
([[(4-amino-6-methyl-3-oxoheptanoyl)amino]acetyl]oxy)acetic acid
-
-
2-trifluorocinnamic acid
-
-
-
3-(3-pyridyl)acrylic acid
-
-
-
3-benzoylpropionic acid
-
-
4-Phenyl-3-butenoic acid
4-phenyl-3-butenoic acid methyl ester
-
at 0.01 mg/ml inhibition in vivo of proliferation of Ras-transformed WB rat liver epithelial cells, inhibitor leads to increased gap junction communication in the WB-Ras cells, overview
4-phenylbut-3-enoic acid
-
PBA
5-(acetylamino)-4-oxo-6-phenyl-2-hexenoic acid
-
-
5-(acetylamino)-4-oxo-6-phenyl-2-hexenoic acid methyl ester
-
-
6-O-palmitoyl-L-ascorbate
-
-
acetyl-D-Leu-OCH2COOH
-
-
acetyl-D-Phe-Gly
-
-
acetyl-D-Phe-OCH2COOH
-
-
acetyl-DL-Phe-OCH2COOH
-
-
acetyl-Gly-OCH2COOH
-
-
acetyl-L-Leu-OCH2COOH
-
-
acetyl-L-Phe-D-Phe-Gly
-
-
acetyl-L-Phe-Gly
-
only substrate for alpha-hydroxylation
acetyl-L-Phe-OCH2COOH
-
-
adrenocorticotrophic hormone(1-10)
-
-
alpha-N-acetyl-adrenocorticotrophic hormone(1-14)
-
-
ascorbate
-
above 0.4 mM for the purified enzyme, above 1.5 mM for the enzyme in crude extract
Cinnamic acid
-
extensive dialysis of bifunctional PAM incubated with cinnamate yielded inactive enzyme unable to catalyze the production of glyoxylate from hippuric acid
Cu2+
-
at higher concentration
D-Tyr-Pro-Gly-Gly
-
inhibition of activity with D-Tyr-Val-Gly
D-Tyr-Val-Gly
decanoyloxyacetic acid
-
-
diethyldithiocarbamate
Disulfiram
-
-
dithiothreitol
-
-
endorphin(51-61)NH2
-
i.e. pro-adrenocorticotrophic hormone(1-10)
-
endorphins
-
and proendorphins
-
ferrocyanide
-
inhibiting above 0.6 mM
gamma-keto acid
-
-
-
glutathione ethyl ester
-
-
glycolate
-
-
Glyoxylate 2-pyridylhydrazone
-
-
Glyoxylate 4-carboxyphenylhydrazone
-
-
Glyoxylate benzylhydrazone
-
-
glyoxylate phenylhydrazone
-
strongly
Glyoxylate phenylsemicarbazone
-
-
Glyoxylate phenylthiosemicarbazone
-
-
Glyoxylate semicarbazone
-
-
glyoxylate thiosemicarbazone
-
-
glyoxylate-N-hexyl-4-carbamoylphenylhydrazone
-
strongly
hippuric acid
-
-
imidazole
-
-
Monoethyl fumarate
-
mechanism-based inhibition
N,S-dibenzoyl-L-cysteine
-
-
N-(benzoyl)-D-serine
-
-
N-(thiobenzoyl)-(D,L)-alanine
-
-
N-acetyl-D-Leu-OCH2COOH
-
competitive inhibitor
N-acetyl-D-Phe-Gly
-
-
N-acetyl-L-Leu-OCH2COOH
-
competitive inhibitor
N-acetyl-L-Phe-OCH2COOH
-
competitive inhibitor
N-benzylglycine
-
-
N-Carboxymethyl N'-phenylhydrazone
-
weak inhibition
N-dansyl-4-aminocinnamate
-
a fluorescent molecule, an inactivator
-
N-phenylthiohydantoic acid
-
-
NaN3
-
-
O-(phenylcarbamoyl)glycolic acid
-
-
O-acetyl-D-mandelyl-Gly
-
-
O-benzamidoglycolic acid
-
-
p-hydroxymercuribenzoate
-
slightly
peptides
-
especially those with COOH-terminal glycine residues
perdeuterated cinnamic acid
-
-
-
phenylpropynoic acid
-
i.e. phenylpropiolic acid
-
phosphate
-
-
Pro-Leu-Gly hydroxamic acid
-
-
S-(2-phenylthioacetyl)thioglycolic acid
S-(3-phenylthiopropionyl)thioglycolic acid
S-(4-methylthiobenzoyl)thioglycolic acid
S-(4-methylthiobenzoyl)thioglycolic acid ethyl ester
-
-
S-(phenylthiocarbamoyl)-3-mercaptopropionic acid
S-(phenylthiocarbamoyl)thioglycolic acid
S-(thiobenzoyl)-(R,S)-thiolactic acid
-
-
S-(thiobenzoyl)-4-mercapto-4-cyanopentanoic acid
-
-
S-(thiobenzoyl)thioglycolic acid
S-(thiolauroyl)thioglycolic acid
-
-
S-phenylmercaptoacetic acid
Salicylhydroxamic acid
-
-
sulfite
-
irreversible inactivation is Cu2+-dependent
trans-Benzoylacrylic acid
-
mechanism-based inhibition
trans-cinnamic acid
-
-
Urocanic acid
-
-
[(1R,2S)-2-phenylcyclopropyl]acetic acid
-
-
[(4-Methoxybenzoyl)oxy]acetic acid
-
-
[(phenylacetyl)oxy]acetic acid
-
-
[(phenylacetyl)sulfanyl]acetic acid
-
-
[[(2S)-2-(acetylamino)-3-phenylpropanoyl]oxy]acetic acid
-
-
[[(2S)-2-(acetylamino)-3-phenylpropanoyl]sulfanyl]acetic acid
-
-
[[(2S)-2-([[(2-aminopropanoyl)amino]acetyl]amino)-3-phenylpropanoyl]oxy]acetic acid
-
-
[[(2S)-2-amino-3-phenylpropanoyl]oxy]acetic acid
-
-
[[(2S)-pyrrolidin-2-ylcarbonyl]oxy]acetic acid
-
-
[[(acetylamino)acetyl]oxy]acetic acid
-
-
[[N-(4-amino-6-methyl-3-oxoheptanoyl)-L-phenylalanyl]oxy]acetic acid
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
form 1, AE-I, activates below pH 6.0
chloride
-
form 1, AE-I, activates below pH 6.0
dexamethasone
-
increases gene expression 1.6fold, northern blot analysis
Iodide
-
form 1, AE-I, activates below pH 6.0
O2
-
substrate binding triggers oxygen activation in peptidylglycine monooygenase
additional information
-
enzyme expression is induced by androgen-deprivation therapy
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19
2,6-difluorohippuric acid
-
apparent KM
1.4
2-aminohippuric acid
-
apparent KM
0.86
2-hydroxyhippuric acid
-
apparent KM
3.4
2-iodohippuric acid
-
apparent KM
4.3
2-methylhippuric acid
-
apparent KM
0.22
2-propylmercaptoacetylglycine
-
apparent KM
0.036
2-pyridylmercaptoacetylglycine
-
apparent KM
1.2
3-(2-furyl)acryloylglycine
-
apparent KM
2.2
3-chlorohippuric acid
-
apparent KM
0.3
3-indolylacetylglycine
-
apparent KM
1.4
3-methylhippuric acid
-
apparent KM
0.038
3-phenylthiopropionylglycine
-
apparent KM
2.6
4-aminohippuric acid
-
apparent KM
0.52
4-bromohippuric acid
-
apparent KM
0.62
4-chlorohippuric acid
-
apparent KM
1.2
4-ethylhippuric acid
-
apparent KM
1.7
4-hydroxyhippuric acid
-
apparent KM
0.82
4-methoxyhippuric acid
-
apparent KM
1.8
4-methylhippuric acid
-
apparent KM
0.11
4-nitrobenzoyl-Gly-Gly
-
apparent KM
0.47
4-nitrohippuric acid
-
apparent KM
0.16
4-phenylbutyrylglycine
-
apparent KM
1.2
4-propylhippuric acid
-
apparent KM
0.77
4-trifluoromethylhippuric acid
-
apparent KM
0.15
5-phenylpentanoylglycine
-
apparent KM
0.051
6-phenylhexanoylglycine
-
apparent KM
0.1
8-phenyloctanoylglycine
-
apparent KM
1400
acetyl-D-alanine
-
apparent KM
1.5
acetyl-Gly-Gly
-
apparent KM
0.00035 - 1.39
acetyl-Tyr-Phe-Gly
0.2
alanyl-L-phenylalanylglycine
-
pH and temperature not specified in the publication
4
alanyl-L-prolylglycine
-
pH and temperature not specified in the publication
0.035
alanylglycyl-L-phenylalanylglycine
-
pH and temperature not specified in the publication
3
alanylglycyl-L-prolylglycine
-
pH and temperature not specified in the publication
0.018
alanylglycylvalyl-L-phenylalanylglycine
-
pH and temperature not specified in the publication
0.96
alanylglycylvalyl-L-prolylglycine
-
pH and temperature not specified in the publication
0.292
alpha-hydroxyhippuric acid
-
0.4 mM ferrocyanide, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4
84
benzoyl-D-alanine
-
apparent KM
35
CBZ-D-alanine
-
apparent KM
0.59
CBZ-glycine
-
apparent KM
0.42
chloroacetyl-Gly-Gly
-
apparent KM
0.23
cinnamoylglycine
-
apparent KM
0.033 - 0.289
D-Tyr-Pro-Gly-Gly
0.007 - 0.22
D-Tyr-Val-Gly
0.022 - 0.143
dabsyl-Gly-Phe-Gly
0.00091 - 0.8
dansyl-D-Tyr-Val-Gly
0.0033 - 0.0194
dansyl-L-Tyr-L-Val-Gly
4.5
dansyl-Tyr-Phe-Gly
-
recombinant mutant AE-II, form 2
3.5
dansyl-Tyr-Phe-Gly(OH)
-
reaction product of step 1, substrate of step 2; recombinant mutant AE-II, form 2
0.003
dansyl-Tyr-Val-Gly
-
recombinant enzyme
0.03 - 0.26
ferrocyanide
2.1
gamma-Glu-Gly-Gly
-
pH 6.0, 37C
0.13
glutathione
-
pH 6.0, 37C
2.78
H2O2
-
100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, H2O2
0.61 - 1.3
hippuric acid
0.078
hippuryl-Gly
-
apparent KM
0.31
hippuryl-Gly-Gly
-
apparent KM
0.94
hydrocinnamoylglycine
-
apparent KM
0.28
isocaproylglycine
-
apparent KM
0.28
L-ascorbate
-
+ 0.02 mM N-dansyl-Tyr-Phe-Gly
6.5
L-phenylalanylglycine
-
pH and temperature not specified in the publication
0.88
L-prolylglycine
-
pH and temperature not specified in the publication
0.31
L-pyroglutamyl-Gly
-
apparent KM
0.035
leukotriene C4
-
pH 6.0, 37C
2.7
N-(2-furoyl)glycine
-
apparent KM
1.6
N-(2-thienylcarbonyl)glycine
-
apparent KM
0.011
N-(4-amino-6-methyl-3-oxoheptanoyl)-L-phenylalanylglycine
-
pH and temperature not specified in the publication
0.86
N-(4-amino-6-methyl-3-oxoheptanoyl)glycylglycine
-
pH and temperature not specified in the publication
4.1
N-(alpha-methylhydrocinnamoyl)glycine
-
apparent KM
0.16 - 0.33
N-(Phenylacetyl)glycine
0.12
N-acetyl-Arg-Gly-Gly
-
100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly
0.02
N-acetyl-Gln-Lys-Glu-Ser-Thr-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly
-
100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly
0.0079
N-acetyl-L-Phe-Gly
-
pH not specified in the publication, temperature not specified in the publication
0.015 - 0.016
N-acetyl-L-phenylalanylglycine
0.13
N-acetyl-Leu-Arg-Gly-Gly
-
100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly
0.024
N-acetyl-Leu-Arg-Leu-Arg-Gly-Gly
-
100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly
0.018
N-acetyl-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly
-
100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly
0.0012 - 0.0098
N-acetyl-Tyr-Val-Gly
9.3 - 18
N-acetylglycine
1.2
N-acetylglycylglycine
-
pH and temperature not specified in the publication
1.3
N-benzoylglycine
-
-
2.3
N-butyrylglycine
-
pH 6.0, 37C
0.0025
N-Dan-Tyr-Val-Gly
-
PHM activity in the soluble fraction of control and intermittent hypoxia brain stem ectracts
0.0032
N-dansyl-Tyr-Phe-Gly
-
+ 2 mM L-ascorbate
0.031
N-dansyl-Tyr-Val-D-Ala
-
apparent KM
0.0077
N-dansyl-Tyr-Val-Gly
-
pH 6.5, 37C
0.1 - 0.2
N-decanoylglycine
0.42 - 0.58
N-hexanoylglycine
0.2
N-octanoylglycine
-
pH 6.0, 37C
2.4
N-propionylglycine
-
pH 6.0, 37C
4.1
N-trifluoroacetylglycine
-
-
0.0011
N-trinitrophenyl-D-Tyr-Val-Gly
Frog
-
pH 6.0, 37C, recombinant enzyme
0.9
N-[(2-phenylcyclopropyl)carbonyl]glycine
-
apparent KM
0.059
N-[(benzylmercapto)carbonyl]glycine
-
apparent KM
0.007
N-[3-(1-leucylpyrrolidin-2-yl)-3-oxopropanoyl]-L-phenylalanylglycine
-
pH and temperature not specified in the publication
0.55
N-[3-(1-leucylpyrrolidin-2-yl)-3-oxopropanoyl]glycylglycine
-
pH and temperature not specified in the publication
1.9
nicotinuric acid
-
apparent KM
0.17
O-acetyl-L-mandelyl-Gly
-
pH not specified in the publication, temperature not specified in the publication
0.1 - 658.1
O2
0.009 - 0.018
peptidylglycine
0.36
phenylhydantoic acid
-
apparent KM
0.19
phenylmercaptoacetylglycine
-
apparent KM
0.024
phenylthioacetylglycine
-
apparent KM
1.1
S-(1,2-dicarboxyethyl)-glutathione
-
pH 6.0, 37C
0.027
S-(4-nitrobenzyl)-glutathione
-
pH 6.0, 37C
0.067
S-butyl-glutathione
-
pH 6.0, 37C
0.016
S-decyl-glutathione
-
pH 6.0, 37C
0.23
S-ethyl-glutathione
-
pH 6.0, 37C
0.035
S-hexyl-glutathione
-
pH 6.0, 37C
0.27
S-methyl-glutathione
-
pH 6.0, 37C
0.032
S-octyl-glutathione
-
pH 6.0, 37C
0.11
S-propyl-glutathione
-
pH 6.0, 37C
3.7
tBOC-Gly
-
apparent KM
0.3
tBOC-Gly-Gly
-
apparent KM
0.0044 - 0.0068
Trinitrophenyl-D-Tyr-Val-Gly
0.6
ubiquitin
-
100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5 - 9.2
(25R)-3beta-hydroxycholest-5-en-27-oate
0.02
acetyl-Tyr-Phe-Gly
-
-
51
alpha-hydroxyhippuric acid
-
0.4 mM ferrocyanide, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4
0.13
D-Tyr-Val-Gly
Frog
-
pH 7.5, 37C, recombinant enzyme
17
dansyl-Gly-Gly-Ser-CO-NH-CH2-COOH
-
pH 6.0, 37C, recombinant enzyme
0.08 - 13.8
dansyl-L-Tyr-L-Val-Gly
0.143 - 0.983
dansyl-Tyr-Val-Gly
0.02 - 39.1
hippuric acid
37
HOOC-NH-CH2-COOH
-
pH 6.0, 37C, recombinant enzyme
0.0035 - 15
N-acetyl-Tyr-Val-Gly
3 - 14.5
N-benzoylglycine
0.35 - 5
N-dansyl-Tyr-Val-Gly
1.4
N-trinitrophenyl-D-Tyr-Val-Gly
Frog
-
pH 6.0, 37C, recombinant enzyme
additional information
additional information
-
intrinsic rate constants for wild-type and mutant Y318F enzyme
-