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7-keto-cholesterol + O2
?
-
-
-
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
?
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
?
beta-cholestanol + O2
?
13% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
?
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
20% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
10% activity compared to cholesterol
-
-
?
cholestanol + O2
?
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
41% activity compared to cholesterol
-
-
?
ergosterol + O2
?
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
22% activity compared to cholesterol
-
-
?
pregnenolone + O2
?
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
?
-
about 55% of the activity with cholesterol, cholesterol oxidase II
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
-
-
-
?
cholestanol + O2
?
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
cholesterol + O2
cholest-5-en-3-one + H2O2
dehydro-epi-androsterone + O2
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
41.4% of the activity with cholesterol
-
?
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
-
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
22.4% of activity with chelesterol
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
trans-androsterone + O2
?
-
-
-
-
?
trans-dehydroandrosterone + O2
?
-
-
-
-
?
additional information
?
-
no activity with ergosterol
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
13.2% of activity with cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
19.7% of activity with cholesterol
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
about 30% of the activity with cholesterol, cholesterol oxidase I
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
dehydro-epi-androsterone + O2
?
-
about 35% of the activity with cholesterol, cholesterol oxidase I
-
-
?
dehydro-epi-androsterone + O2
?
-
about 5% of the activity with cholesterol, cholesterol oxidase II
-
-
?
pregnenolone + O2
?
-
-
-
-
?
pregnenolone + O2
?
-
about 60% of the activity with cholesterol, cholesterol oxidase I
-
-
?
pregnenolone + O2
?
-
about 60% of the activity with cholesterol, cholesterol oxidase II
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
10% of activity with cholesterol
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
about 10% of the activity with cholesterol, cholesterol oxidase I
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
about 10% of the activity with cholesterol, cholesterol oxidase II
-
-
?
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0.8 - 1.2
3beta-hydroxy-androst-5-en-17-one
0.27
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.4
dehydroepiandrosterone
-
-
0.8
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.9 - 1.2
trans-dehydroandrosterone
0.8
3beta-hydroxy-androst-5-en-17-one
-
-
0.8
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
1.2
3beta-hydroxy-androst-5-en-17-one
-
-
1.2
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.02
cholestanol
-
-
0.2
cholestanol
-
assay relies on detection of H2O2 formation
0.2
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.03
cholesterol
-
pH 7.0, 37°C, cholesterol oxidase II
0.04
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.07
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.11
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.14
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.14
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.2
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.25
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.47
cholesterol
-
H69A mutant enzyme, 50 mM phosphate buffer, pH 7.5, 1% thesit, 1% 2-propanol
1.1
cholesterol
-
pH 7.0, 37°C, cholesterol oxidase I
0.165
O2
-
-
0.45
O2
-
H69A mutant enzyme, 50 mM phosphate buffer, pH 7.5, 1% thesit, 1% 2-propanol
0.2
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.4
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.4
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.9
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
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0.8
3beta-hydroxy-5-androsten-17-one
0.8
3beta-hydroxy-androst-5-en-17-one
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
28 - 278
5-Cholesten-3-one
0.8
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.8 - 1
trans-dehydroandrosterone
0.8
3beta-hydroxy-5-androsten-17-one
-
assay relies on detection of H2O2 formation
0.8
3beta-hydroxy-5-androsten-17-one
-
H2O2 production assayed, 100 mM phosphate buffer
28
5-Cholesten-3-one
-
H69A mutant enzyme, 5-cholesten-3-one isomerization, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
250
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
278
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
40
cholestanol
-
assay relies on detection of H2O2 formation
40
cholestanol
-
H2O2 production assayed, 100 mM phosphate buffer
40
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.55
cholesterol
-
H69A mutant enzyme, steady state, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
0.8
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation, decreasing length of C17 chain affects turnover negatively
1.6
cholesterol
-
H69A mutant enzyme, cholesterol oxidation, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
43
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
48
cholesterol
-
50 mM phosphate buffer, pH 7.5
48
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
48
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
48.1
cholesterol
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
56
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
57
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
67
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
67
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
105
cholesterol
-
50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol
21
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
21
pregnenolone
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
21
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
35
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.8
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
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Uwajima, T.; Yagi, H.; Terado, O.
Properties of crystalline 3beta-hydroxysteroid oxidase of Brevibacterium sterolicum
Agric. Biol. Chem.
38
1149-1156
1974
Brevibacterium sterolicum
-
brenda
Uwajima, T.; Terada, O.
On the kinetics of cholesterol oxidase from Brevibacterium sterolicum in the presence of detergents
Agric. Biol. Chem.
42
1453-1454
1978
Brevibacterium sterolicum
-
brenda
Uwajima, T.; Yagi, H.; Nakamura, S.; Terada, O.
Isolation and crystallization of extracellular 3beta-hydroxysteroid oxidase of Brevibacterium sterolicum nov. Sp.
Agric. Biol. Chem.
37
2345-2350
1973
Brevibacterium sterolicum
-
brenda
Smith, A.G.; Brooks, C.J.W.
Cholesterol oxidases: properties and applications
J. Steroid Biochem.
7
705-713
1976
Streptomyces violascens, Brevibacterium sterolicum, Nocardia erythropolis
brenda
Li, J.; Vrielink, A.; Brick, P.; Blow, D.M.
Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases
Biochemistry
32
11507-11515
1993
Brevibacterium sterolicum
brenda
Croteau, N.; Vrielink, A.
Crystallization and preliminary x-ray analysis of cholesterol oxidase from Brevibacterium sterolicum containing covalently bound FAD
J. Struct. Biol.
116
317-319
1996
Brevibacterium sterolicum
brenda
Gadda, G.; Wels, G.; Pollegioni, L.; Zucchelli, S.; Amrosius, D.; Pilone, M.S.; Ghisla, S.
Characterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum
Eur. J. Biochem.
250
369-376
1997
Brevibacterium sterolicum, Streptomyces hygroscopicus
brenda
Pollegioni, L.; Gadda, G.; Ambrosius, D.; Ghisla, S.; Pilone, M.S.
Cholesterol oxidase from Streptomyces hycroscopicus and Brevibacterium sterolicum: effect of surfactants and organic solvents on activity
Biotechnol. Appl. Biochem.
30
27-33
1999
Brevibacterium sterolicum, Streptomyces hygroscopicus
brenda
Pollegioni, L.; Wels, G.; Pilone, M.S.; Ghisla, S.
Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum
Eur. J. Biochem.
264
140-151
1999
Brevibacterium sterolicum, Streptomyces hygroscopicus
brenda
MacLachlan, J.; Wotherspoon, A.T.L.; Ansell, R.O.; Brooks, C.J.W.
Cholesterol oxidase: sources, physical properties and analytical applications
J. Steroid Biochem. Mol. Biol.
72
169-195
2000
Streptomyces lavendulae, Streptomyces violascens, Pimelobacter simplex, Brevibacterium sterolicum, Streptomyces sp., Comamonas testosteroni, Rhodococcus equi, Corynebacterium cholesterolicum, Mycobacterium sp., Nocardia erythropolis, Nocardia rhodochrous, Nocardia sp., Pseudomonas sp., Rhodococcus erythropolis, Rhodococcus sp., Schizophyllum commune, Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces lividans, Rhodococcus sp. GK1
brenda
Motteront, L.; Pilonet, M.S.; Molla, G.; Ghisla, S.; Pollegioni, L.
Cholesterol oxidase from Brevibacterium sterolicum
J. Biol. Chem.
276
18024-18030
2001
Brevibacterium sterolicum
brenda
Doukyu, N.; Aono, R.
Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200
Appl. Microbiol. Biotechnol.
57
146-152
2001
Brevibacterium sterolicum, Burkholderia cepacia, Streptomyces sp., Nocardia erythropolis, Pseudomonas sp., Burkholderia cepacia ST-200
brenda
Fujishiro, K.; Uchida, H.; Shimokawa, K.; Nakano, M.; Sano, F.; Ohta, T.; Kayahara, N.; Aisaka, K.; Uwajima, T.
Purification and properties of a new Brevibacterium sterolicum cholesterol oxidase produced by E. coli MM294/pnH10
FEMS Microbiol. Lett.
215
243-248
2002
Brevibacterium sterolicum
brenda
Basu, A.K.; Chattopadhyay, P.; Roychoudhuri, U.; Chakraborty, R.
Development of cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on oxygen electrode for the determination of total cholesterol in food samples
Bioelectrochemistry
70
375-379
2006
Brevibacterium sterolicum
brenda
Caldinelli, L.; Iametti, S.; Barbiroli, A.; Bonomi, F.; Fessas, D.; Molla, G.; Pilone, M.S.; Pollegioni, L.
Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin
J. Biol. Chem.
280
22572-22581
2005
Brevibacterium sterolicum
brenda
Piubelli, L.; Pedotti, M.; Molla, G.; Feindler-Boeckh, S.; Ghisla, S.; Pilone, M.S.; Pollegioni, L.
On the oxygen reactivity of flavoprotein oxidases: An oxygen access tunnel and gate in Brevibacterium sterolicum cholesterol oxidase
J. Biol. Chem.
283
24738-24747
2008
Brevibacterium sterolicum
brenda
Aparicio, J.F.; Martin, J.F.
Microbial cholesterol oxidases: bioconversion enzymes or signal proteins?
Mol. Biosyst.
4
804-809
2008
Arthrobacter sp., Streptomyces sp., Rhodococcus equi, Rhodococcus erythropolis, Rhodococcus rhodochrous, Schizophyllum commune, Brevibacterium sterolicum (P22637)
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Caldinelli, L.; Iametti, S.; Barbiroli, A.; Fessas, D.; Bonomi, F.; Piubelli, L.; Molla, G.; Pollegioni, L.
Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD
Protein Sci.
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2008
Brevibacterium sterolicum
brenda
Vrielink, A.; Ghisla, S.
Cholesterol oxidase: biochemistry and structural features
FEBS J.
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2009
Brevibacterium sterolicum, Streptomyces hygroscopicus
brenda
Pollegioni, L.; Piubelli, L.; Molla, G.
Cholesterol oxidase: biotechnological applications
FEBS J.
276
6857-6870
2009
Streptomyces violascens, Arthrobacter sp., Rhodococcus equi, Mycobacterium sp., Nocardia erythropolis, Nocardia rhodochrous, Pseudomonas spp., Schizophyllum commune, uncultured Gammaproteobacteria bacterium, Streptoverticillum cholesterolicum, Brevibacterium sterolicum (P22637), uncultured Gammaproteobacteria bacterium Y-134
brenda
Doukyu, N.
Characteristics and biotechnological applications of microbial cholesterol oxidases
Appl. Microbiol. Biotechnol.
83
825-837
2009
Arthrobacter sp., Arthrobacter sp. (Q56DL0), Arthrobacter sp. IM79, Brevibacterium sterolicum (P22637), Burkholderia cepacia (Q93RE1), Burkholderia cepacia ST-200 (Q93RE1), Burkholderia pseudomallei, Burkholderia thailandensis (Q2T0X3), Chromobacterium sp. (B5MGF8), Corynebacterium cholesterolicum, Corynebacterium urealyticum (B1VGH1), Corynebacterium urealyticum DSM 7109 (B1VGH1), Mycobacterium leprae (Q59530), Mycobacterium tuberculosis (P9WMV9), Mycobacterium tuberculosis H37Rv (P9WMV9), Nocardia farcinica (Q5YRJ2), Nocardia farcinica IFM 10152 (Q5YRJ2), Nocardia rhodochrous, Nocardia sp., Paraburkholderia xenovorans (Q13UN0), Pimelobacter simplex, Pseudomonas aeruginosa, Pseudomonas sp., Pseudomonas sp. COX629, Rhodococcus equi (A0A3S5YA89), Rhodococcus equi 103S (A0A3S5YA89), Rhodococcus erythropolis (A9QAE7), Schizophyllum commune, Streptomyces avermitilis (Q93H76), Streptomyces coelicolor (Q9L0H6), Streptomyces fradiae, Streptomyces griseus (A7RA76), Streptomyces natalensis (Q9EW96), Streptomyces sp. (P12676), Streptomyces sp. (Q55020), Streptomyces violascens, Streptoverticillium cholesterolieum, uncultured Gammaproteobacteria bacterium, uncultured Gammaproteobacteria bacterium Y-134
brenda
Volonte, F.; Pollegioni, L.; Molla, G.; Frattini, L.; Marinelli, F.; Piubelli, L.
Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli
BMC Biotechnol.
10
33
2010
Brevibacterium sterolicum
brenda