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Literature summary for 1.1.3.6 extracted from
- Cholesterol oxidase: biotechnological applications (2009), FEBS J., 276, 6857-6870.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | recombinant expression in plants gives insect resistance | Arthrobacter sp. |
| agriculture | recombinant expression in plants gives insect resistance | Mycobacterium sp. |
| agriculture | recombinant expression in plants gives insect resistance | Nocardia rhodochrous |
| agriculture | recombinant expression in plants gives insect resistance | Nocardia erythropolis |
| agriculture | recombinant expression in plants gives insect resistance | Rhodococcus equi |
| agriculture | recombinant expression in plants gives insect resistance | Schizophyllum commune |
| agriculture | recombinant expression in plants gives insect resistance | Pseudomonas spp. |
| agriculture | recombinant expression in plants gives insect resistance | Streptomyces violascens |
| agriculture | recombinant expression in plants gives insect resistance | uncultured Gammaproteobacteria bacterium |
| agriculture | recombinant expression in plants gives insect resistance | Streptoverticillum cholesterolicum |
| agriculture | recombinant expression in plants gives insect resistance | Brevibacterium sterolicum |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Arthrobacter sp. |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Mycobacterium sp. |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Nocardia rhodochrous |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Nocardia erythropolis |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Rhodococcus equi |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Schizophyllum commune |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Pseudomonas spp. |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Streptomyces violascens |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | uncultured Gammaproteobacteria bacterium |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Streptoverticillum cholesterolicum |
| biotechnology | biocatalysis, industrial steroid drug production, steroid production as diagnostic tool | Brevibacterium sterolicum |
| diagnostics | analytic tool for determining cholesterol in various samples | Arthrobacter sp. |
| diagnostics | analytic tool for determining cholesterol in various samples | Mycobacterium sp. |
| diagnostics | analytic tool for determining cholesterol in various samples | Nocardia rhodochrous |
| diagnostics | analytic tool for determining cholesterol in various samples | Nocardia erythropolis |
| diagnostics | analytic tool for determining cholesterol in various samples | Rhodococcus equi |
| diagnostics | analytic tool for determining cholesterol in various samples | Schizophyllum commune |
| diagnostics | analytic tool for determining cholesterol in various samples | Pseudomonas spp. |
| diagnostics | analytic tool for determining cholesterol in various samples | Streptomyces violascens |
| diagnostics | analytic tool for determining cholesterol in various samples | uncultured Gammaproteobacteria bacterium |
| diagnostics | analytic tool for determining cholesterol in various samples | Streptoverticillum cholesterolicum |
| diagnostics | analytic tool for determining cholesterol in various samples | Brevibacterium sterolicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | engineered ChOx to improve activity and alter specificity | Rhodococcus equi |
| additional information | engineered ChOx to improve activity and alter specificity | Streptomyces violascens |
| additional information | engineered ChOx to improve activity and alter specificity | Streptoverticillum cholesterolicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Schizophyllum commune | - |
- |
| extracellular | - |
Pseudomonas spp. | - |
- |
| extracellular | - |
Streptomyces violascens | - |
- |
| extracellular | - |
Streptoverticillum cholesterolicum | - |
- |
| extracellular | - |
Brevibacterium sterolicum | - |
- |
| intracellular | - |
Arthrobacter sp. | 5622 | - |
| intracellular | - |
Mycobacterium sp. | 5622 | - |
| intracellular | - |
Nocardia rhodochrous | 5622 | - |
| intracellular | - |
Nocardia erythropolis | 5622 | - |
| intracellular | - |
Rhodococcus equi | 5622 | - |
| membrane | - |
Arthrobacter sp. | 16020 | - |
| membrane | - |
Mycobacterium sp. | 16020 | - |
| membrane | - |
Nocardia rhodochrous | 16020 | - |
| membrane | - |
Nocardia erythropolis | 16020 | - |
| membrane | - |
Rhodococcus equi | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | - |
- |
- |
| Brevibacterium sterolicum | P22637 | - |
- |
| Mycobacterium sp. | - |
- |
- |
| Nocardia erythropolis | - |
- |
- |
| Nocardia rhodochrous | - |
- |
- |
| Pseudomonas spp. | - |
- |
- |
| Rhodococcus equi | - |
high levels of ChOx activity, significant similarities to putative ChOxs encoded by Mycobacterium tuberculosis and Mycobacterium leprae | - |
| Schizophyllum commune | - |
- |
- |
| Streptomyces violascens | - |
- |
- |
| Streptoverticillum cholesterolicum | - |
- |
- |
| uncultured Gammaproteobacteria bacterium | - |
Y-134 | - |
| uncultured Gammaproteobacteria bacterium Y-134 | - |
Y-134 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparison of relative activity of ChOx from different sources | Rhodococcus equi |
| additional information | - |
comparison of relative activity of ChOx from different sources | uncultured Gammaproteobacteria bacterium |
| additional information | - |
comparison of relative activity of ChOx from different sources | Brevibacterium sterolicum |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Arthrobacter sp. |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Mycobacterium sp. |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Nocardia rhodochrous |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Nocardia erythropolis |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Rhodococcus equi |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Schizophyllum commune |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Pseudomonas spp. |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Streptomyces violascens |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | uncultured Gammaproteobacteria bacterium |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Streptoverticillum cholesterolicum |
| additional information | - |
comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) | Brevibacterium sterolicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-keto-cholesterol + O2 | - |
Rhodococcus equi | ? | - |
? |  |
| 7-keto-cholesterol + O2 | - |
uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| 7-keto-cholesterol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
| 7-keto-cholesterol + O2 | - |
uncultured Gammaproteobacteria bacterium Y-134 | ? | - |
? | |
| 7alpha-hydroxy-cholesterol + O2 | - |
Rhodococcus equi | 7alpha-hydroxy-cholest-4-en-3-one + H2O2 | - |
? | |
| 7alpha-hydroxy-cholesterol + O2 | - |
uncultured Gammaproteobacteria bacterium | 7alpha-hydroxy-cholest-4-en-3-one + H2O2 | - |
? | |
| 7alpha-hydroxy-cholesterol + O2 | - |
Brevibacterium sterolicum | 7alpha-hydroxy-cholest-4-en-3-one + H2O2 | - |
? | |
| 7beta-hydroxy-cholesterol + O2 | - |
Rhodococcus equi | 7beta-hydroxy-cholest-4-en-3-one + H2O2 | - |
? | |
| 7beta-hydroxy-cholesterol + O2 | - |
uncultured Gammaproteobacteria bacterium | 7beta-hydroxy-cholest-4-en-3-one + H2O2 | - |
? | |
| 7beta-hydroxy-cholesterol + O2 | - |
Brevibacterium sterolicum | 7beta-hydroxy-cholest-4-en-3-one + H2O2 | - |
? | |
| beta-sitosterol + O2 | - |
Rhodococcus equi | ? | - |
? | |
| beta-sitosterol + O2 | - |
uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| beta-sitosterol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
| beta-sitosterol + O2 | - |
uncultured Gammaproteobacteria bacterium Y-134 | ? | - |
? | |
| cholestanol + O2 | - |
Rhodococcus equi | ? | - |
? | |
| cholestanol + O2 | - |
uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| cholestanol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
| cholesterol + O2 | - |
Arthrobacter sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Mycobacterium sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Nocardia rhodochrous | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Rhodococcus equi | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Schizophyllum commune | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Pseudomonas spp. | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Streptomyces violascens | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
uncultured Gammaproteobacteria bacterium | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Streptoverticillum cholesterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
uncultured Gammaproteobacteria bacterium Y-134 | cholest-4-en-3-one + H2O2 | - |
? | |
| ergosterol + O2 | - |
Rhodococcus equi | ? | - |
? | |
| ergosterol + O2 | - |
uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| ergosterol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
| ergosterol + O2 | - |
uncultured Gammaproteobacteria bacterium Y-134 | ? | - |
? | |
| pregnenolone + O2 | - |
Rhodococcus equi | ? | - |
? | |
| pregnenolone + O2 | - |
uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| pregnenolone + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
| pregnenolone + O2 | - |
uncultured Gammaproteobacteria bacterium Y-134 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3beta-hydroxysterol oxidase | - |
Arthrobacter sp. |
| 3beta-hydroxysterol oxidase | - |
Mycobacterium sp. |
| 3beta-hydroxysterol oxidase | - |
Nocardia rhodochrous |
| 3beta-hydroxysterol oxidase | - |
Nocardia erythropolis |
| 3beta-hydroxysterol oxidase | - |
Rhodococcus equi |
| 3beta-hydroxysterol oxidase | - |
Schizophyllum commune |
| 3beta-hydroxysterol oxidase | - |
Pseudomonas spp. |
| 3beta-hydroxysterol oxidase | - |
Streptomyces violascens |
| 3beta-hydroxysterol oxidase | - |
uncultured Gammaproteobacteria bacterium |
| 3beta-hydroxysterol oxidase | - |
Streptoverticillum cholesterolicum |
| 3beta-hydroxysterol oxidase | - |
Brevibacterium sterolicum |
| cholesterol oxidase | - |
Arthrobacter sp. |
| cholesterol oxidase | - |
Mycobacterium sp. |
| cholesterol oxidase | - |
Nocardia rhodochrous |
| cholesterol oxidase | - |
Nocardia erythropolis |
| cholesterol oxidase | - |
Rhodococcus equi |
| cholesterol oxidase | - |
Schizophyllum commune |
| cholesterol oxidase | - |
Pseudomonas spp. |
| cholesterol oxidase | - |
Streptomyces violascens |
| cholesterol oxidase | - |
uncultured Gammaproteobacteria bacterium |
| cholesterol oxidase | - |
Streptoverticillum cholesterolicum |
| cholesterol oxidase | - |
Brevibacterium sterolicum |
| ChOx | - |
Arthrobacter sp. |
| ChOx | - |
Mycobacterium sp. |
| ChOx | - |
Nocardia rhodochrous |
| ChOx | - |
Nocardia erythropolis |
| ChOx | - |
Rhodococcus equi |
| ChOx | - |
Schizophyllum commune |
| ChOx | - |
Pseudomonas spp. |
| ChOx | - |
Streptomyces violascens |
| ChOx | - |
uncultured Gammaproteobacteria bacterium |
| ChOx | - |
Streptoverticillum cholesterolicum |
| ChOx | - |
Brevibacterium sterolicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | both as a covalently or noncovalently bound cofactor | Arthrobacter sp. | |
| FAD | both as a covalently or noncovalently bound cofactor | Mycobacterium sp. | |
| FAD | both as a covalently or noncovalently bound cofactor | Nocardia rhodochrous | |
| FAD | both as a covalently or noncovalently bound cofactor | Nocardia erythropolis | |
| FAD | both as a covalently or noncovalently bound cofactor | Rhodococcus equi | |
| FAD | both as a covalently or noncovalently bound cofactor | Schizophyllum commune | |
| FAD | both as a covalently or noncovalently bound cofactor | Pseudomonas spp. | |
| FAD | both as a covalently or noncovalently bound cofactor | Streptomyces violascens | |
| FAD | both as a covalently or noncovalently bound cofactor | Streptoverticillum cholesterolicum | |
| FAD | both as a covalently or noncovalently bound cofactor | Brevibacterium sterolicum | |
| FMN | covalently bound | uncultured Gammaproteobacteria bacterium | |
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