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Literature summary for 1.1.3.6 extracted from

  • MacLachlan, J.; Wotherspoon, A.T.L.; Ansell, R.O.; Brooks, C.J.W.
    Cholesterol oxidase: sources, physical properties and analytical applications (2000), J. Steroid Biochem. Mol. Biol., 72, 169-195.
    View publication on PubMed

Application

Application Comment Organism
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Pseudomonas sp.
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Pimelobacter simplex
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Mycobacterium sp.
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Nocardia sp.
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Rhodococcus sp.
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Rhodococcus erythropolis
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Streptomyces sp.
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Nocardia rhodochrous
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Nocardia erythropolis
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Rhodococcus equi
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Streptomyces lividans
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Schizophyllum commune
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Streptomyces hygroscopicus
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Streptomyces griseocarneus
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Streptomyces lavendulae
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Streptomyces violascens
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Brevibacterium sterolicum
medicine determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments Corynebacterium cholesterolicum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans Brevibacterium sterolicum

Inhibitors

Inhibitors Comment Organism Structure
fenpropimorph irreversible inhibition; morpholine derivative, 50 mg/l, 50% inhibition, instantenous inhibition Comamonas testosteroni
fenpropimorph morpholine derivative, 50 mg/l, 50% inhibition, instantenous inhibition; reversible non-competitive Nocardia erythropolis
fenpropimorph morpholine derivative, 50 mg/l, 50% inhibition, instantenous inhibition; reversible non-competitive Schizophyllum commune
fenpropimorph morpholine derivative, 50 mg/l, 50% inhibition, instantenous inhibition; reversible competitive mechanism Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Rhodococcus equi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Pseudomonas sp.
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Pimelobacter simplex
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Mycobacterium sp.
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Comamonas testosteroni
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Nocardia sp.
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Rhodococcus sp.
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Rhodococcus erythropolis
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces sp.
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Nocardia rhodochrous
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Nocardia erythropolis
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Rhodococcus equi
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces lividans
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Schizophyllum commune
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces hygroscopicus
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces griseocarneus
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces lavendulae
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces violascens
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Brevibacterium sterolicum
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Corynebacterium cholesterolicum
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Rhodococcus sp. GK1
-
cholest-5-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Brevibacterium sterolicum
-
-
-
Comamonas testosteroni
-
-
-
Corynebacterium cholesterolicum
-
-
-
Mycobacterium sp.
-
-
-
Nocardia erythropolis
-
-
-
Nocardia rhodochrous
-
-
-
Nocardia sp.
-
-
-
Pimelobacter simplex
-
-
-
Pseudomonas sp.
-
-
-
Rhodococcus equi
-
-
-
Rhodococcus erythropolis
-
-
-
Rhodococcus sp.
-
solated from soil
-
Rhodococcus sp. GK1
-
solated from soil
-
Schizophyllum commune
-
-
-
Streptomyces griseocarneus
-
-
-
Streptomyces hygroscopicus
-
-
-
Streptomyces lavendulae
-
-
-
Streptomyces lividans
-
-
-
Streptomyces sp.
-
-
-
Streptomyces violascens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, ion-exchange chromatography, gel filtration Rhodococcus equi
choleterylglycine-CM-cellulose Pseudomonas sp.
DEAE-cellulose, cholesterol affinity column, Sephadex G-150 Pseudomonas sp.
partially purified by precipitation with ammonium sulfate Rhodococcus sp.

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Pseudomonas sp.
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Pimelobacter simplex
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Mycobacterium sp.
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia sp.
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Rhodococcus sp.
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Rhodococcus erythropolis
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces sp.
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia rhodochrous
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia erythropolis
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Rhodococcus equi
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces lividans
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Schizophyllum commune
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces hygroscopicus
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces griseocarneus
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces lavendulae
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces violascens
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Brevibacterium sterolicum
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Corynebacterium cholesterolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Pseudomonas sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Pimelobacter simplex cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Mycobacterium sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Comamonas testosteroni cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus erythropolis cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia rhodochrous cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia erythropolis cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus equi cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces lividans cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Schizophyllum commune cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces hygroscopicus cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces griseocarneus cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces lavendulae cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces violascens cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Corynebacterium cholesterolicum cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus sp. GK1 cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Pseudomonas sp. cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Pimelobacter simplex cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Mycobacterium sp. cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Comamonas testosteroni cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia sp. cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus sp. cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus erythropolis cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces sp. cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia rhodochrous cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia erythropolis cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus equi cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces lividans cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Schizophyllum commune cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces hygroscopicus cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces griseocarneus cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces lavendulae cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces violascens cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Brevibacterium sterolicum cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Corynebacterium cholesterolicum cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus sp. GK1 cholest-5-en-3-one + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Rhodococcus equi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.2 membrane bound enzyme Rhodococcus sp.
7 7.5 extracellular enzyme Rhodococcus sp.