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Literature summary for 1.1.3.6 extracted from

  • Uwajima, T.; Yagi, H.; Terado, O.
    Properties of crystalline 3beta-hydroxysteroid oxidase of Brevibacterium sterolicum (1974), Agric. Biol. Chem., 38, 1149-1156.
No PubMed abstract available
Search for more literature for 1.1.3.6

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1 mM, 100% inhibition, 0.1 mM, 62% inhibition Brevibacterium sterolicum
HgCl2 1 mM, 68% inhibition, 0.1 mM, 38% inhibition Brevibacterium sterolicum
p-chloromercuribenzoate 1 mM, 20% inhibition, 0.1 mM, 14% inhibition Brevibacterium sterolicum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information contains no metal ions Brevibacterium sterolicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 gel filtration, sedimentation equilibrium Brevibacterium sterolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Brevibacterium sterolicum
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacterium sterolicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brevibacterium sterolicum

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Brevibacterium sterolicum
a

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Brevibacterium sterolicum
-

Storage Stability

Storage Stability Organism
0-5°C, crystallized enzyme in lyophilized state, in the dark, stable for at least 10 months Brevibacterium sterolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-cholestan-3beta-ol + O2 13.2% of activity with cholesterol Brevibacterium sterolicum 5alpha-cholestan-3-one + H2O2
-
 ?
beta-sitosterol + O2 19.7% of activity with cholesterol Brevibacterium sterolicum stigmast-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Brevibacterium sterolicum cholest-5-en-3-one + H2O2
-
 ?
dehydroepiandrosterone + O2 41.4% of the activity with cholesterol Brevibacterium sterolicum androst-5-en-3,17-dione + H2O2
-
 ?
pregnenolone + O2 22.4% of activity with chelesterol Brevibacterium sterolicum 5-pregnen-3,20-dione + H2O2
-
 ?
stigmasterol + O2 10% of activity with cholesterol Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.0, 30 min, stable Brevibacterium sterolicum
60
-
 pH 7.0, 30 min, 19% loss of activity Brevibacterium sterolicum
70
-
 pH 7, 30 min, about 94% loss of activity Brevibacterium sterolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Brevibacterium sterolicum

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 about 50% of maximal activity at pH 5 and 9.5 Brevibacterium sterolicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 37°C, 30 min, stable Brevibacterium sterolicum
5 10 37°C, 30 min, stable Brevibacterium sterolicum

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Brevibacterium sterolicum
FAD 1 mol of FAD per mol of protein Brevibacterium sterolicum