EC Number   |
|---|
   1.1.3.6 | - |
| 1.1.3.6 | crystal screen based on sparse matrix formulation, using 500 mM ammonium sulfate, 1.0 M lithium sulfate and 10 mM sodium citrate |
| 1.1.3.6 | crystals of recombinant His-tagged enzyme, vapor diffusion by hanging drop, space group P21 |
| 1.1.3.6 | crystals of the H447Q/E361Q mutant protein are grown by hanging-drop vapor diffusion method, high-resolution crystal structure of mutant enzyme H447Q/E361Q with glycerol and without glycerol |
| 1.1.3.6 | hanging-drop vapour-diffusion method using polyethylene glycol as a precipitating agent. The crystals belong to space group P3(1)21 with unit-cell parameters a = b = 119.6, c = 101.1 A, and has one subunit in the asymmetric unit |
| 1.1.3.6 | mutant enzymes N485D and N485L, hanging drop vapor diffusion method, using 9-11% (w/v) polyethylene glycol 8000, 75 mM MnSO4 and 100 mM sodium cacodylate pH 5.2 |
| 1.1.3.6 | purified nontagged and His6-tagged enzyme reduced with dithionite under aerobic conditions and in the presence of the substrate 2-propanol under both aerobic and anaerobic conditions, vapour diffusion using the hanging-drop method, mixing of 7 mg/ml protein in 50 mM HEPES pH 7.0 with reservoir solution containing 7% PEG 8000, 100 mM cacodylate, pH 5.2, 125 mM MnSO4, followed by microseeding in 12% PEG 8000, 100 mM sodium cacodylate, pH 5.2, 125 mM MnSO4, X-ray diffraction structure determination and analysis at 1.12-1.34 A resolution |
| 1.1.3.6 | rod shaped crystals with bright yellow color |
| 1.1.3.6 | structure of substrate-enzyme complex, 1.8 A resolution, substrate binds in internal cavity which is totally sealed from solvent |
| 1.1.3.6 | structure of the F359W mutant enzyme, hanging drop vapor diffusion method. In the atomic-resolution structure of F359W, the indole ring of the tryptophan completely fills the tunnel and is observed in only a single conformation |
