EC Number   |
|---|
   1.1.3.6 | - |
| 1.1.3.6 | ChoL, a fragment of a cholesterol oxidase is expressed in Escherichia coli BL21(DE3) plysS cells |
| 1.1.3.6 | cholesterol oxidase II, expression in Escherichia coli MM294/pnH10 |
| 1.1.3.6 | cloning, sequencing and analysis of partial segment of cholesterol oxidase, the pTZ57R/T vector is used for molecular cloning |
| 1.1.3.6 | construction of a constitutive high-level-expression vector for the genus Bifidobacterium to express cholesterol oxidase from Streptomyces coelicola. The promoter region of the 16S rRNA gene is amplified by inverse PCR and used for the construction of pBES16PR. The optimal ribosome-binding site for Bifidobacterium is incorporated in pBES16PR. pBES16PR-CHOL with the structural gene for cholesterol oxidase under the control of the 16S rRNA promoter is used to transform Bifidobacterium longum. The gene is successfully expressed and high level of cholesterol oxidase activity is obtained in Bifidobacterium longum |
| 1.1.3.6 | expressed in Escherichia coli BL21 cells |
| 1.1.3.6 | expressed in Escherichia coli BL21(DE3)pLysS cells |
| 1.1.3.6 | expression in Escherichia coli |
| 1.1.3.6 | expression in Escherichia coli strain BL21-CodonPlus (DE3)-RP of the enzyme with and without N- or C-terminal His-tags, the hydrophobicity of the enzyme is reduced by His-tags |
| 1.1.3.6 | expression in Escherichia coli, His-tagged enzyme |
