2.3.1.304: poly[(R)-3-hydroxyalkanoate] polymerase

This is an abbreviated version!
For detailed information about poly[(R)-3-hydroxyalkanoate] polymerase, go to the full flat file.

Reaction

Synonyms

BP-M-CPF4, broad-range class I PhaCAc, CLAOCE_21140, CLAOCE_21150, CLAOCE_21150/21140, class I PHA synthase, Class I PhaC, class I PhaCRe, class I PHB synthase, Class I poly(R)-hydroxyalkanoic acid synthase, class I polyhydroxyalkanoate synthase, class I polyhydroxybutyrate synthase, Class I synthase, Class II PHA polymerizing enzyme, class II PHA synthase, Class II PhaC, class II PhaC1, class III PHA synthase, class III PHB synthase, class III polyhydroxyalkanoate synthase, class III polyhydroxybutyrate synthase, class III synthase, class IV PHA synthase, H16_A1437, HPTL0263, HPTL0635, HPTL1376, intracellular polyhydroxyalkanoate synthase, P(3HB) synthase, PHA polymerase, PHA synthase, PHA synthase 1, PHA synthase I, PHA synthase II, PHA synthase III, PhaC, PhaC type II, PhaC-II, PhaC1, PhaC1P-5, PhaC1Pp, PhaC1Ps, PhaC1SG, PhaC2, PhaC2P-5, PhaC2Ps, PhaC2SG, PhaCAc, PhaCAv, phaCBP-M-CPF4, PhaCCc, PhaCCn-CAT, PhaCCs, PhaCCs-CAT, phaCCv, PhaCPhaEAv, PhaCRe, PhaE, PhaEC, PhaECAv, PhaRCBm, PhaRCYB4, PHB synthase, PhbC, PhbCRe, phbC_2, phbE, poly(3-hydroxybutyrate) synthase, poly(hydroxyalkanoic acid) synthase, poly-3-hydroxybutyrate synthase, poly-beta-hydroxybutyrate synthase, polyhydroxyalkanoate (PHA) synthase, polyhydroxyalkanoate synthase, polyhydroxyalkanoate synthase 1, polyhydroxyalkanoate synthase synthase, polyhydroxyalkanoic acids synthase, polyhydroxyalkanoic synthase I, polyhydroxyalkanoic synthase II, polyhydroxyalkanoic synthase III, polyhydroxybutyrate synthase, Q667_12980, TH-1_PHB synthase_HPTL_0263, TH-1_PHB synthase_HPTL_0635, TH-1_PHB synthase_HPTL_1376, type I PHA synthase, type I polyhydroxyalkanoate synthase, type II PHA synthase, type II PhaC1, type II polyhydroxyalkanoate synthase, type II Pseudomonas PHA synthase 1, type III PHA synthase, type-III PHA synthase, YdcS

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.304 poly[(R)-3-hydroxyalkanoate] polymerase

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Results	in table
10	Activating Compound
35	AA Sequence
36	Application
87	Cloned(Commentary)
1	Crystallization (Commentary)
3	Expression
98	General Information
1	General Stability
1	IC50 Value
8	Inhibitors
10	kcat/KM [mM/s]
3	Ki Value [mM]
30	KM Value [mM]
13	Localization
4	Metals/Ions
13	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
304	Organism
4	Pathway
12	pH Optimum
2	pH Range
1	pI Value
2	Posttranslational Modification
236	Protein Variants
21	Purification (Commentary)
5	Reaction
167	Reference
8	Source Tissue
10	Specific Activity [micromol/min/mg]
183	Substrates and Products (Substrate)
47	Subunits
406	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
21	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 2.3.1.304 - poly[(R)-3-hydroxyalkanoate] polymerase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.151

Burkholderia sp. USM (JCM 15050)

-

native enzyme, at pH 8.0 and 30°C

760327

0.243

Burkholderia sp. USM (JCM 15050)

-

recombinant enzyme expressed in Cupriavidus necator PHB-4, at pH 8.0 and 30°C

760327

0.48

Thermus thermophilus

-

crude extract, at pH 8.5 and 70°C

761890

1

Pseudomonas sp. SG4502

-

wild type enzyme isoform PhaC1SG, at pH 8.0 and 30°C

760429

2.5

Pseudomonas sp. SG4502

-

mutant enzyme S324T/Q480K of isoform PhaC1SG, at pH 8.0 and 30°C

760429

2050

Thermus thermophilus

-

after 4271fold purification, at pH 8.5 and 70°C

761890

27.6

Ectothiorhodospira shaposhnikovii

Q9F5P8; Q9F5P9, Q9F5P9

-

685488

3 - 8

Cupriavidus necator

-

pooled monomeric and dimeric fractions of Strep2-PhaCRe, 30°C, pH not specified in the publication

718922

36

Cupriavidus necator

-

purified recombinant Strep2-PhaCRe, 30°C, pH not specified in the publication

718922

additional information

Escherichia coli

-

193259 cpm/mg

684934