Literature summary for 2.3.1.304 extracted from

Tajima, K.; Han, X.; Satoh, Y.; Ishii, A.; Araki, Y.; Munekata, M.; Taguchi, S.
In vitro synthesis of polyhydroxyalkanoate (PHA) incorporating lactate (LA) with a block sequence by using a newly engineered thermostable PHA synthase from Pseudomonas sp. SG4502 with acquired LA-polymerizing activity (2012), Appl. Microbiol. Biotechnol., 94, 365-376 .

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Protein Variants

Protein Variants	Comment	Organism
S324T/Q480K	the mutant shows increased activity compared to the wild type enzyme	Pseudomonas sp. SG4502

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n	Pseudomonas sp. SG4502	-	CoA + poly[(R)-3-hydroxyalkanoate]n+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. SG4502	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1	-	wild type enzyme isoform PhaC1SG, at pH 8.0 and 30°C	Pseudomonas sp. SG4502
2.5	-	mutant enzyme S324T/Q480K of isoform PhaC1SG, at pH 8.0 and 30°C	Pseudomonas sp. SG4502

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n	-	Pseudomonas sp. SG4502	CoA + poly[(R)-3-hydroxyalkanoate]n+1	-	?
(R)-lactoyl-CoA + (3R)-3-hydroxybutyryl-CoA	-	Pseudomonas sp. SG4502	CoA + poly(lactate-co-3-hydroxybutyrate)	-	?

Subunits

Subunits	Comment	Organism
?	x * 62600, isoform PhaC1SG, calculated from amino acid sequence	Pseudomonas sp. SG4502
?	x * 63000, isoform PhaC2SG, calculated from amino acid sequence	Pseudomonas sp. SG4502

Synonyms

Synonyms	Comment	Organism
PHA synthase	-	Pseudomonas sp. SG4502
PhaC1SG	isoform	Pseudomonas sp. SG4502
PhaC2SG	isoform	Pseudomonas sp. SG4502

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Release 2023.1 (January 2023)