Literature summary for 2.3.1.304 extracted from

Lau, N.S.; Sudesh, K.
Revelation of the ability of Burkholderia sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer (2012), AMB Express, 2, 41 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Cupriavidus necator PHB-4	Burkholderia sp. USM (JCM 15050)

Organism

Organism	UniProt	Comment	Textmining
Burkholderia sp. USM (JCM 15050)	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.151	-	native enzyme, at pH 8.0 and 30°C	Burkholderia sp. USM (JCM 15050)
0.243	-	recombinant enzyme expressed in Cupriavidus necator PHB-4, at pH 8.0 and 30°C	Burkholderia sp. USM (JCM 15050)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxybutyrate]n	-	Burkholderia sp. USM (JCM 15050)	CoA + poly[(R)-3-hydroxybutyrate]n+1	-	?
additional information	the transformant Cupriavidus necator PHB-4 harbouring Burkholderia sp. enzyme gene accumulates poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with 4-hydroxybutyrate monomer as high as up to 87 mol% from sodium 4-hydroxybutyrate. The wild type Burkholderia sp. does not have the ability to produce this copolymer	Burkholderia sp. USM (JCM 15050)	?	-	-

Synonyms

Synonyms	Comment	Organism
PHA synthase	-	Burkholderia sp. USM (JCM 15050)
PhaC	-	Burkholderia sp. USM (JCM 15050)

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Release 2023.1 (January 2023)