Information on EC 2.3.1.304 - poly[(R)-3-hydroxyalkanoate] polymerase

for references in articles please use BRENDA:EC2.3.1.304
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
This is the key enzyme in the biosynthesis of polyhydroxyalkanoates (PHA), linear polyesters produced by bacteria as a means of carbon and energy storage . The enzyme catalyses the stereoselective, covalent linkage of (3R)-3-hydroxyacyl-CoA thioesters in a transesterification reaction with concomitant release of coenzyme A. The growing polymer is attached to a conserved active site L-cysteine residue. Three types of PHA synthases have been proposed based on their substrate specificity and enzyme structure. Type I and type III synthases preferentially polymerize short chain hydroxyalkanoate monomers containing 3-5 carbon atoms [1,2]. The difference between these two types is that type I synthases are composed of only a single subunit (PhaC), whereas type III synthases are composed of two different subunits, PhaC and PhaE [3,5]. Type II synthases are also composed of a single subunit (PhaC), but preferentially polymerize monomers containing more than 5 carbon atoms .
Specify your search results
Select one or more organisms in this record: ?
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
BP-M-CPF4, broad-range class I PhaCAc, CLAOCE_21140, CLAOCE_21150, CLAOCE_21150/21140, class I PHA synthase, Class I PhaC, class I PhaCRe, class I PHB synthase, Class I poly(R)-hydroxyalkanoic acid synthase, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n = CoA + poly[(R)-3-hydroxyalkanoate]n+1
show the reaction diagram
-
-
-
-
(R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n = [(R)-3-hydroxybutanoate]n+1 + CoA
show the reaction diagram
Tyr36 is catalytically essential
-
-
-
3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n = [(R)-3-hydroxyacyl]n+1 + CoA
show the reaction diagram
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n = [(R)-3-hydroxybutanoate]n+1 + CoA
show the reaction diagram
-
-
-
-