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2.1.1.355: [histone H3]-lysine9 N-trimethyltransferase

This is an abbreviated version!
For detailed information about [histone H3]-lysine9 N-trimethyltransferase, go to the full flat file.

Word Map on EC 2.1.1.355

Reaction

3 S-adenosyl-L-methionine +

a [histone H3]-L-lysine9
= 3 S-adenosyl-L-homocysteine +
a [histone H3]-N6,N6,N6-trimethyl-L-lysine9

Synonyms

Clr4, dG9a, Dim-5, Dim-5 histone lysine methyltransferase, Dim-5 histone-3 lysine-9 methyltransferase, Dim5, DOT1L, EC 2.1.1.43, EHMT1, EHMT2, ESET, G9a, G9a histone methyltransferase, G9a HMT, G9a lysine 9 histone H3 methyltransferase, G9a-like protein lysine methyltransferase, G9a/G9a-like protein lysine dimethyltransferase complex, GLP, H3-K9 methyltransferase, H3K9 histone methyltransferase, H3K9 histone methyltransferases, H3K9 HMT, H3K9 methyltransferase, H3K9-specific histone methyltransferase, H3K9-specific SET domain methyltransferase, histone H3 Lys79 methyltransferase, histone H3 lysine 9 methyltransferase, histone H3 lysine methyltransferase G9a, histone H3 methyltransferase, histone H3K9 methyltransferase, histone H3K9 trimethylase, histone lysine methyltransferase, histone lysine methyltransferase G9a, histone methyltransferase, histone methyltransferase G9a, HKMT, HKMTase, HMTase, KHMTase, KMT1A, KMT1B, KMT1C, KMT1D, KMT1F, lysine methyltransferase G9a, lysine-preferring HMTase, MT8, PRDM2, PRDM8, SETDB1, SU (VAR)3-9, SU(VAR)3-9, SUV39H1, SUVR4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.355 [histone H3]-lysine9 N-trimethyltransferase

Engineering

Engineering on EC 2.1.1.355 - [histone H3]-lysine9 N-trimethyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C1197A
mutation of conserved residue within the SET domain, loss of catalytic activity
F326A
I290A
the mutant shows significantly reduced activity compared to the wild type enzyme
L284A
the mutant shows significantly reduced activity compared to the wild type enzyme
L329A
the mutant shows significantly reduced activity compared to the wild type enzyme
R282A
the mutation dramatically decreases the enzyme activity
deltaANK
-
is inactive as a coactivator
deltaSET
-
lacks the enzymatic activity in vitro, synergistic coactivator function observed with glucocorticoid receptor interacting protein 1, coactivator-associated arginine methyltransferase 1 is reduced but not eliminated
H1166K
-
lacks the enzymatic activity in vitro, synergistic coactivator function observed with glucocorticoid receptor interacting protein 1, coactivator-associated arginine methyltransferase 1 is reduced but not eliminated
D209A
-
in solution experiments with the wild type histone H3 sequence, the mutant shows a 100fold reduced activity as compared to wild-type Dim-5
D209S
-
in solution experiments with the wild type histone H3 sequence, the mutant shows a 100fold reduced activity as compared to wild-type Dim-5
E227A
-
in solution experiments with the wild type histone H3 sequence, the mutant shows a 10fold reduced activity as compared to wild-type Dim-5
E227S
-
in solution experiments with the wild type histone H3 sequence, the mutant shows a 10fold reduced activity as compared to wild-type Dim-5
F281W
-
shows catalytic activity
F281Y
-
shows catalytic activity
Y178F
-
shows catalytic activity, although the methylation rates of this variant are low
Y178V
-
shows catalytic activity
additional information