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Literature summary for 2.1.1.355 extracted from

  • Lee, D.Y.; Northrop, J.P.; Kuo, M.H.; Stallcup, M.R.
    Histone H3 lysine 9 methyltransferase G9a is a transcriptional coactivator for nuclear receptors (2006), J. Biol. Chem., 281, 8476-8485.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information functions as a coactivator for nuclear receptors, cooperating synergistically with nuclear receptor coactivators glucocorticoid receptor interacting protein 1, coactivator-associated arginine methyltransferase 1, and p300 in transient transfection assays, synergy depends strongly on the arginine-specific protein methyltransferase activity of CARM1 but does not absolutely require the enzymatic activity of G9a and is specific to CARM1 and G9a among various protein methyltransferases, link between histone arginine and lysine methylation and a mechanism for controlling whether G9a functions as a corepressor or coactivator Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplified EcoRI-XhoI insert encoding the murine long form of G9a cloned into the EcoRI-XhoI sites of pSG5.HA and pSG5.FLAG, PCR-amplified EcoRI-SalI inserts of G9a residues 1-333, 72-333, 330-690, 685-1018, 936-1263 (deltaANK), 1-1088 (deltaSET), 730-1263, and 464-1263 cloned into the EcoRI-SalI sites of the vector pM for Gal4 DNA binding domain fusions and into the EcoRI-XhoI sites of pSG5.HA or pSG5.FLAG, expression in CV-1 and Cos-7 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
deltaANK is inactive as a coactivator Mus musculus
deltaSET lacks the enzymatic activity in vitro, synergistic coactivator function observed with glucocorticoid receptor interacting protein 1, coactivator-associated arginine methyltransferase 1 is reduced but not eliminated Mus musculus
H1166K lacks the enzymatic activity in vitro, synergistic coactivator function observed with glucocorticoid receptor interacting protein 1, coactivator-associated arginine methyltransferase 1 is reduced but not eliminated Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
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Synonyms

Synonyms Comment Organism
histone H3 lysine 9 methyltransferase
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Mus musculus