Information on EC 5.5.1.4 - inositol-3-phosphate synthase

New: Word Map on EC 5.5.1.4
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria

EC NUMBER
COMMENTARY hide
5.5.1.4
-
RECOMMENDED NAME
GeneOntology No.
inositol-3-phosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate
show the reaction diagram
D-glucose 6-phosphate = L-myo-inositol 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
-
-
-
-
elimination/addition
-
-
intramolecular
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
-
-
1D-myo-inositol hexakisphosphate biosynthesis IV (Dictyostelium)
-
-
Biosynthesis of antibiotics
-
-
di-myo-inositol phosphate biosynthesis
-
-
Inositol phosphate metabolism
-
-
Metabolic pathways
-
-
mycothiol biosynthesis
-
-
myo-inositol biosynthesis
phosphatidylinositol biosynthesis I (bacteria)
-
-
Streptomycin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
1D-myo-inositol-3-phosphate lyase (isomerizing)
Requires NAD+, which dehydrogenates the -CHOH- group to -CO- at C-5 of the glucose 6-phosphate, making C-6 into an active methylene, able to condense with the -CHO at C-1. Finally, the enzyme-bound NADH reconverts C-5 into the -CHOH- form.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-95-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene MIPS1
UniProt
Manually annotated by BRENDA team
aquatic fern
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
chloroplast and cytosolic isoforms
-
-
Manually annotated by BRENDA team
Gleichenia glauca
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
expression is upregulated under cold and heat stress
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isoform MIPS1
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose 6-phosphate
5-deoxy-L-myo-inositol 1-phosphate
show the reaction diagram
D-2-Deoxy-2-fluoroglucose 6-phosphate
5-Deoxy-5-fluoro-myo-inositol 1-phosphate
show the reaction diagram
D-glucose 6-phosphate
1D-inositol 3-phosphate
show the reaction diagram
D-glucose 6-phosphate
1D-myo-inositol 3-phosphate
show the reaction diagram
D-glucose 6-phosphate
1L-myo-inositol 1-phosphate
show the reaction diagram
D-glucose 6-phosphate
1L-myo-inositol-1-phosphate
show the reaction diagram
-
-
-
-
?
D-Glucose 6-phosphate
?
show the reaction diagram
D-glucose 6-phosphate
D-myo-inositol 3-phosphate
show the reaction diagram
D-Glucose 6-phosphate
L-myo-Inositol 1-phosphate
show the reaction diagram
D-Mannose 6-phosphate
myo-Inositol 1-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate
1D-inositol 3-phosphate
show the reaction diagram
D-glucose 6-phosphate
1D-myo-inositol 3-phosphate
show the reaction diagram
D-glucose 6-phosphate
1L-myo-inositol 1-phosphate
show the reaction diagram
-
first and rate-limiting step in biosynthesis of all inositol-conatining compounds
-
-
?
D-glucose 6-phosphate
1L-myo-inositol-1-phosphate
show the reaction diagram
-
-
-
-
?
D-Glucose 6-phosphate
?
show the reaction diagram
D-Glucose 6-phosphate
L-myo-Inositol 1-phosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
-
almost equally active as NAD+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
modulates enzyme expression increasing or decreasing it dependent on growth conditions, overview
Co2+
-
absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+
Cu2+
inhibition
Hg2+
inhibition
NaCl
-
modulates enzyme expression increasing or decreasing it dependent on growth conditions, overview
phosphate
-
modulates enzyme expression increasing or decreasing it dependent on growth conditions, overview
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
1,5-Anhydro-D-glucitol 6-phosphate
-
-
1-Deoxy-1-(phosphonomethyl)myo-2-inosose
-
competitive
2-Deoxy-2-fluoroglucose 6-phosphate
2-deoxy-D-glucitol
-
-
2-deoxy-D-glucitol 6-(E)-vinylhomophosphonate
-
binding structure, interaction with active site residues, overview
-
2-Deoxy-D-glucitol 6-phosphate
-
-
2-deoxy-D-glucose 6-phosphate
2-deoxy-glucitol-6-phosphate
-
the inhibitor induces folding of the catalytic domain
-
2-Deoxy-myo-inositol 1-phosphate
-
competitive
choline
-
slight inhibition
D-Allose 6-phosphate
-
-
D-fructose 6-phosphate
D-galactose 6-phosphate
D-gluconate 6-phosphate
-
-
D-glucose 6-phosphate
-
the beta-anomer is preferred as substrate, the alpha-anomer is an inhibitor
D-mannitol 6-phosphate
-
-
D-mannose 6-phosphate
D-sorbitol 6-phosphate
-
-
dihydroxyacetone phosphate
-
-
diphosphate
-
inhibition occurs only in the presence of NH4+. Inhibition is pH-dependent. The inhibition of enzyme activity by diphosphate, at pH 7.7, is reversed in decreasing order of effectiveness by Mn2+, Fe3+, Mg2+, Co2+, Cu2+, Ca2+, Ba2+ and Zn2+
Endogeneous inhibitor from latex serum
-
-
-
glucitol 6-phosphate
Inositol
lithium
mannitol
Mg2+
-
inhibitory only at nonphysiological concentrations
myo-2-Inosose 1-phosphate
NaBH4
-
partial inactivation in presence of NAD+. No inactivation in absence of NAD+
NH4HCO3
p-Substituted mercuribenzoate
-
1 mM, 93% inhibition in presence of 1 mM NADH
pyridoxal 5'-phosphate
-
-
sorbitol
Trinitrobenzene sulphonate
-
-
valnoctamide
-
valproate derivative with anticonvulsant effect. Drastic inhibition of enzyme at 1 mM in crude brain homogenate, competitive
Valproate
valrocemide
-
valproate derivative with anticonvulsant effect. Drastic inhibition of enzyme at 1 mM in crude brain homogenate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
-
stimulates
NaCl
leaf L-myo-inositol 1-phosphate concentration increases 40fold during the first 6 h of salt stress (exposure to 300 mM NaCl)
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.15
2-deoxy-D-glucose 6-phosphate
pH 7, 37C, Vmax: 280 nM
0.12 - 4.47
D-glucose 6-phosphate
0.035 - 2.85
glucose 6-phosphate
0.0051 - 1.95
NAD+
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0817 - 9.6
D-glucose 6-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 0.233
D-glucose 6-phosphate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00067
2-deoxy-D-glucitol
-
pH 5.5
0.18
valnoctamide
-
pH 7.6, 37C
0.21
Valproate
-
brain, post mortem
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
Methanothermobacter thermautotrophicum
-
crude enzyme, in 50mM Bicine buffer (pH 8.5), at 60C
0.013
-
purified recombinant refolded enzyme, with cofactor NADP+
0.015
-
purified recombinant refolded enzyme, with cofactor NAD+
0.018
-
purified native enzyme, with cofactor NADP+
0.02
-
purified native enzyme, with cofactor NAD+
0.05
-
value about, culture condition: 2 days, 30C
0.06
-
value about, culture condition: 3 days, 30C
0.1
-
value about, culture condition: 1 day, 30C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
-
7.3 - 7.8
-
-
7.8 - 8.5
-
-
8 - 8.5
-
mutant bearing amino acids 174-210 of Porteresia coarctata enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
-
34% of maximal activity at pH 9.0, low activity below pH 5.0, inactive above pH 11.0
6.5 - 9.5
-
pH 6.5: about 50% of maximal activity, pH 8.5: about 95% of maximal activity
7 - 7.5
optimal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 37
-
-
35
native enzyme
35 - 37
-
-
35
-
native enzyme
35 - 37
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
-
increasing activity from 20C to 40 C, rapid loss of 60% activity at 50C, inactive at 60C
76 - 100
-
76C: about 40% of maximal activity, 100C: about 50% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.08
calculated from amino acid sequence
7.5
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest expression of isoform MIPS1
Manually annotated by BRENDA team
-
dorsal root ganglia
Manually annotated by BRENDA team
-
low expression
Manually annotated by BRENDA team
-
isoform gammac predominates in intestine
Manually annotated by BRENDA team
-
moderately high expression
Manually annotated by BRENDA team
-
serum
Manually annotated by BRENDA team
-
moderately high expression
Manually annotated by BRENDA team
-
young
Manually annotated by BRENDA team
-
isoenzyme OsMIPS1-1; isoenzyme OsMIPS1-2; isoenzyme OsMIPS1-3: OsMIPS1-3 shows a more or less constitutive expression in different abiotic stresses in shoots, roots, panicle stages, and various developing seed stages; isoenzyme OsMIPS2: expression is flower-specific
Manually annotated by BRENDA team
Gleichenia glauca
-
reproductive pinnule
Manually annotated by BRENDA team
transcript levels increase in unfertilized ovaries and significant amounts are present during the recovery period
Manually annotated by BRENDA team
-
low expression, isoform alpha predominates in spleen
Manually annotated by BRENDA team
additional information