Any feedback?
Literature summary for 5.5.1.4 extracted from
- Inositol-1-phosphate synthase from Archaeoglobus fulgidus is a class II aldolase (2000), Biochemistry, 39, 12415-12423.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.002 mM, 50% inhibition | Archaeoglobus fulgidus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0051 | - |
NAD+ | 90°C, pH 7.5 | Archaeoglobus fulgidus | |
| 0.12 | - |
D-glucose 6-phosphate | 90°C, pH 7.5 | Archaeoglobus fulgidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+ | Archaeoglobus fulgidus | |
| Mg2+ | KM: 0.0242 mM, activates | Archaeoglobus fulgidus | |
| Mn2+ | Km: 0.00077 mM, absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+ | Archaeoglobus fulgidus | |
| Zn2+ | Km: 0.0011 mM, absolute requirement for a divalent metal ion, Zn2+ or Mn2+ are the best activators, followed by Co2+ | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44100 | - |
4 * 44100, SDS-PAGE | Archaeoglobus fulgidus |
| 168000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.8 | - |
- |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | class II aldolase | Archaeoglobus fulgidus | 1D-myo-inositol 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44100, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inositol-1-phosphate synthase | - |
Archaeoglobus fulgidus |
| IPS | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Archaeoglobus fulgidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 76 | 100 | 76°C: about 40% of maximal activity, 100°C: about 50% of maximal activity | Archaeoglobus fulgidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.6 | - |
D-glucose 6-phosphate | - |
Archaeoglobus fulgidus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
at 85°C | Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | pH 6.5: about 50% of maximal activity, pH 8.5: about 95% of maximal activity | Archaeoglobus fulgidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | required | Archaeoglobus fulgidus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
