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Literature summary for 5.5.1.4 extracted from

  • Stieglitz, K.A.; Yang, H.; Roberts, M.F.; Stec, B.
    Reaching for mechanistic consensus across life kingdoms: structure and insights into catalysis of the myo-inositol-1-phosphate synthase (mIPS) from Archaeoglobus fulgidus (2005), Biochemistry, 44, 213-224.
    View publication on PubMed
Search for more literature for 5.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, protein solution containing 8 mg/ml or 16 mg/ml protein, 50 mM Tris, pH 7.5, 1 mM EDTA, versus HEPES, pH 7.5, 2% v/v PEG 400, 2.0 M ammonium sulfate, and 30% w/v PEG 1500 in equal volumes, 2-3 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling Archaeoglobus fulgidus

General Stability

General Stability Organism
5 mM D-glucose 6-phosphate with 1 mM NAD+ stabilizes the enzyme Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Archaeoglobus fulgidus
Mg2+ activates Archaeoglobus fulgidus
Mn2+ activates Archaeoglobus fulgidus
additional information the native enzyme probably contains a bound divalent cation, Mg2+, Mn2+, or Zn2+, which is absolutely required for activity Archaeoglobus fulgidus
Na+ activates Archaeoglobus fulgidus
Zn2+ activates Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus

Reaction

Reaction Comment Organism Reaction ID
D-glucose 6-phosphate = L-myo-inositol 1-phosphate reaction mechanism, active site residues are T228, G229, T230, D261, K274, K278-K373, K306, D332, and K367, mechanism of phosphate and metal binding in the active site, overview Archaeoglobus fulgidus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose 6-phosphate reaction mechanism involves 3 reaction steps: 1. oxidation by NAD+, 2. condensation to a cyclic molecule, 3. reduction by NADH Archaeoglobus fulgidus L-myo-Inositol 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
More structure analysis Archaeoglobus fulgidus
tetramer
-
 Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
MIPS
-
 Archaeoglobus fulgidus
Myo-inositol-1-phosphate synthase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 about Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
98
-
 loss of 50% activity after about 40 min in 50 mM Tris-HCl, pH 8.0 Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD+ binding domain structure, interacts with S149 and Asp261 Archaeoglobus fulgidus