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Literature summary for 5.5.1.4 extracted from

  • Ghosh Dastidar, K.; Maitra, S.; Goswami, L.; Roy, D.; Das, K.P.; Majumder, A.L.
    An insight into the molecular basis of salt tolerance of L-myo-inositol 1-P synthase (PcINO1) from Porteresia coarctata (Roxb.) Tateoka, a halophytic wild rice (2006), Plant Physiol., 140, 1279-1296.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information A 37-amino acid stretch between W174 and S210 is the salt-tolerance determinant domain of enzyme. Deletion of this stretch results in loss of salt tolerance, introduction of stretch into Brassica juncea or Oryza sativa enzyme provides salt tolerance properties Oryza coarctata
additional information a 37-amino acid stretch between W174 and S210 is the salt-tolerance determinant domain of Porteresia coarctata enzyme. Introduction of this stretch into Oryza sativa enzyme provides salt tolerance properties Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 D-glucose 6-phosphate wild-type Oryza coarctata
2.83
-
 D-glucose 6-phosphate mutant lacking amino acids 174-210 Oryza coarctata
3
-
 D-glucose 6-phosphate wild-type, 37°C Oryza sativa
3.19
-
 D-glucose 6-phosphate mutant bearing amino acids 174-210 of Porteresia coarctata enzyme, 37°C Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza coarctata Q7XZE6
-
-
Oryza sativa O64437
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
 Oryza coarctata 1D-myo-inositol 3-phosphate
-
 ?
D-glucose 6-phosphate
-
 Oryza sativa 1D-myo-inositol 3-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Oryza coarctata
37
-
-
 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 wild-type Oryza sativa
8
-
-
 Oryza coarctata
8 8.5 mutant bearing amino acids 174-210 of Porteresia coarctata enzyme Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NAD+ Km value of wild-type 0.166 mM, Km value of mutant lacking amino acids 174-210 0.451 mM Oryza coarctata
NAD+ Km value of wild-type 0.188 mM, Km value of mutant bearing amino acids 174-210 of Porteresia coarctata enzyme 0.451 mM Oryza sativa