Information on EC 3.6.1.15 - nucleoside-triphosphate phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.1.15
-
RECOMMENDED NAME
GeneOntology No.
nucleoside-triphosphate phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a nucleoside triphosphate + H2O = a nucleoside diphosphate + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Purine metabolism
-
-
pyrimidine deoxyribonucleotides biosynthesis from CTP
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis I
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis III
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis IV
-
-
Thiamine metabolism
-
-
oxidative phosphorylation
-
-
SYSTEMATIC NAME
IUBMB Comments
unspecific diphosphate phosphohydrolase
The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. Also hydrolyses nucleoside diphosphates, thiamine diphosphate and FAD. The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9075-51-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PCC 7120
-
-
Manually annotated by BRENDA team
strain 168, gene ytkD
-
-
Manually annotated by BRENDA team
strain 168, gene ytkD
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
HCoV-229E
-
-
Manually annotated by BRENDA team
i.e. JEV
-
-
Manually annotated by BRENDA team
Magnetococcus sp.
strain mc-1
-
-
Manually annotated by BRENDA team
strain mc-1
-
-
Manually annotated by BRENDA team
female C57BL/6J mice
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
no activity in tobacco mosaic virus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
PVA, a poteyvirus
-
-
Manually annotated by BRENDA team
PVX, a potexvirus
-
-
Manually annotated by BRENDA team
strain PAK, genes pilB, pilT and pilU
-
-
Manually annotated by BRENDA team
strain PAK, genes pilB, pilT and pilU
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-didehydro-thymidine triphosphate + H2O
2',3'-didehydro-thymidine diphosphate + phosphate
show the reaction diagram
-
-
-
-
?
3'-azido-2',3'-dideoxythymidine triphosphate + H2O
3'-azido-2',3'-dideoxythymidine diphosphate + phosphate
show the reaction diagram
-
-
-
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
CDP + H2O
CMP + phosphate
show the reaction diagram
CTP + H2O
CDP + phosphate
show the reaction diagram
dADP + H2O
dAMP + phosphate
show the reaction diagram
relative substrate activity toward a variety of nucleoside di- and triphosphate substrates
-
-
?
dATP + H2O
dADP + phosphate
show the reaction diagram
dCDP + H2O
dCMP + phosphate
show the reaction diagram
next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy
-
-
?
dCTP + H2O
dCDP + phosphate
show the reaction diagram
ddCTP + H2O
ddCDP + phosphate
show the reaction diagram
-
-
-
-
?
ddTTP + H2O
ddTDP + phosphate
show the reaction diagram
-
-
-
-
?
dGDP + H2O
dGMP + phosphate
show the reaction diagram
relative substrate activity toward a variety of nucleoside di- and triphosphate substrates
-
-
?
dGTP + H2O
dGDP + phosphate
show the reaction diagram
dITP + H2O
dIDP + phosphate
show the reaction diagram
-
-
-
?
dNTP + H2O
dNDP + phosphate
show the reaction diagram
dTTP + H2O
dTDP + phosphate
show the reaction diagram
-
-
-
-
?
dTTP + H2O
TDP + phosphate
show the reaction diagram
-
-
-
-
?
dUTP + H2O
dUDP + phosphate
show the reaction diagram
-
-
-
-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
GTP + H2O
GDP + phosphate
show the reaction diagram
IDP + H2O
IMP + phosphate
show the reaction diagram
ITP + H2O
IDP + phosphate
show the reaction diagram
L-dATP + H2O
L-dADP + phosphate
show the reaction diagram
-
-
-
-
?
L-dCTP + H2O
CDP + phosphate
show the reaction diagram
-
-
-
-
?
L-dTTP + H2O
L-dTDP + phosphate
show the reaction diagram
-
-
-
-
?
NDP + H2O
NMP + phosphate
show the reaction diagram
-
-
-
-
?
NTP + H2O
NDP + phosphate
show the reaction diagram
RNA + H2O
?
show the reaction diagram
-
removal of the gamma-phosphate
-
-
?
ssDNA + H2O
?
show the reaction diagram
-
removal of gamma-phosphate from nucleotides
-
-
?
ssRNA + H2O
?
show the reaction diagram
-
removal of gamma-phosphate from nucleotides
-
-
?
TDP + H2O
TMP + phosphate
show the reaction diagram
relative substrate activity toward a variety of nucleoside di- and triphosphate substrates
-
-
?
TTP + H2O
TDP + phosphate
show the reaction diagram
UDP + H2O
UMP + phosphate
show the reaction diagram
UTP + H2O
UDP + phosphate
show the reaction diagram
XTP + H2O
XDP + phosphate
show the reaction diagram
-
0.5 mM, 40% relative activity compared to CTP
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + H2O
AMP + phosphate
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
CDP + H2O
CMP + phosphate
show the reaction diagram
CTP + H2O
CDP + phosphate
show the reaction diagram
dADP + H2O
dAMP + phosphate
show the reaction diagram
Q9RXX6
relative substrate activity toward a variety of nucleoside di- and triphosphate substrates
-
-
?
dATP + H2O
dADP + phosphate
show the reaction diagram
dCDP + H2O
dCMP + phosphate
show the reaction diagram
Q9RXX6
next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy
-
-
?
dCTP + H2O
dCDP + phosphate
show the reaction diagram
dGDP + H2O
dGMP + phosphate
show the reaction diagram
Q9RXX6
relative substrate activity toward a variety of nucleoside di- and triphosphate substrates
-
-
?
dGTP + H2O
dGDP + phosphate
show the reaction diagram
dITP + H2O
dIDP + phosphate
show the reaction diagram
Q9RXX6
-
-
-
?
dUTP + H2O
dUDP + phosphate
show the reaction diagram
-
-
-
-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
GTP + H2O
GDP + phosphate
show the reaction diagram
IDP + H2O
IMP + phosphate
show the reaction diagram
ITP + H2O
IDP + phosphate
show the reaction diagram
NTP + H2O
NDP + phosphate
show the reaction diagram
RNA + H2O
?
show the reaction diagram
-
removal of the gamma-phosphate
-
-
?
TDP + H2O
TMP + phosphate
show the reaction diagram
Q9RXX6
relative substrate activity toward a variety of nucleoside di- and triphosphate substrates
-
-
?
TTP + H2O
TDP + phosphate
show the reaction diagram
UDP + H2O
UMP + phosphate
show the reaction diagram
UTP + H2O
UDP + phosphate
show the reaction diagram
XTP + H2O
XDP + phosphate
show the reaction diagram
-
0.5 mM, 40% relative activity compared to CTP
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
5 mM, unable to stimulate enzyme activity
Cs+
-
at concentration below 20 mM
Rb+
-
activates at concentration below 20 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-beta-D-ribofuranosyl-1H-benzotriazole
1-beta-D-ribofuranosyl-4,5,6,7-tetrabromo-1H-benzotriazole
1-beta-D-ribofuranosyl-4,5,6,7-tetramethyl-1H-benzotriazole
1-beta-D-ribofuranosyl-4,7-dibromo-1H-benzotriazole
1-chloroethyl-4,5,6,7-tetrabromo-1H-benzotriazole
1-ethyl-4,5,6,7-tetrabromo-1H-benzotriazole
1-hydroxyethyl-4,5,6,7-tetrabromo-1H-benzotriazole
1-methyl-4,5,6,7-tetrabromo-1H-benzotriazole
1-propyl-4,5,6,7-tetrabromo-1H-benzotriazole
2',3'-didehydro-thymidine triphosphate
-
competitive to ATP
2',3'-dideoxy-5-fluorocytidine triphosphate
-
competitive to ATP
2-chloroethyl-4,5,6,7-tetrabromo-1H-benzotriazole
2-ethyl-4,5,6,7-tetrabromo-1H-benzotriazole
2-hydroxyethyl-4,5,6,7-tetrabromo-1H-benzotriazole
2-methyl-4,5,6,7-tetrabromo-1H-benzotriazole
2-propyl-4,5,6,7-tetrabromo-1H-benzotriazole
3'-azido-2',3'-dideoxythymidine triphosphate
-
competitive to ATP
4,5,6,7-tetrabromo-1H-benzotriazole
4,5-dihydro-8H-6-(N-decyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 30-100 mg/l
4,5-dihydro-8H-6-(N-dodecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 1-3 mg/l
4,5-dihydro-8H-6-(N-dodecylamino)-1-(2'-deoxy-alpha-D-erythropentofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 3-10 mg/l
4,5-dihydro-8H-6-(N-dodecylamino)-1-(2'-deoxy-beta-D-erythropentofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 3-10 mg/l
4,5-dihydro-8H-6-(N-hexadecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 250 mg/l
4,5-dihydro-8H-6-(N-octadecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 5.0 mg/l
4,5-dihydro-8H-6-(N-tetradecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
IC50: 3-10 mg/l
5,6-dichloro-1-beta-D-ribofuranosyl-1H-benzotriazole
5-fluoro-2-selenocytosine
-
IC50: 0.075 mM for NTPase reaction, no influence to helicase activity up to a concentration of 0.5 mM
beta,gamma-methylene-ATP
-
-
Ca2+
-
at high concentrations
CaCl2
in presence of Mn2+, 10 mM MgCl2 inhibits 20%
choline
-
at concentration above 20 mM
Cs+
-
at concentration above 20 mM
dATP
-
competitive to ATP
dCTP
-
competitive to ATP
ddCTP
-
competitive to ATP
ddTTP
-
competitive to ATP
dGTP
-
competitive to ATP
dTTP
-
competitive to ATP
formononetin 7-O-glucoside
-
-
guanidine hydrochloride
-
-
HOCl
-
inhibits the muclear NTPase, taurine acts as antagonist and reduces the hypochlorous acid toxicity
iodoacetamide
-
-
L-dATP
-
competitive to ATP
L-dCTP
-
competitive to ATP
L-ddCTP
-
competitive to ATP
L-dTTP
-
competitive to ATP
Mg2+
-
above 10 mM
MgCl2
in presence of Mn2+, 10 mM MgCl2 inhibits 10%
N-ethylmaleimide
-
-
NaCl
100 mM, inhibits ATP hydrolysis by 30%
O6-benzyl-N7-chloroethylguanine
-
weak inhibition of NTPase activity, enhances helicase activity
O6-benzylguanine
-
weak inhibitor of ATPase and helicase activity
oligo(dA)25
-
above 0.5 mM, inhibits unwinding reaction
-
oligomycin
-
-
p-hydroxymercuribenzoate
-
-
Phenylarsine oxide
-
-
polyA
inhibits to a lesser extent than polyC and polyU
polyC
1 mg/l, 70% inhibition
polyG
inhibits to a lesser extent than polyC and polyU
polyU
1 mg/l, 70% inhibition
Proflavine
-
-
propionate
-
39% relative activity at a concentration of 0.5 mM, 63% relative activity at a concentration of 0.05 mM
Rb+
-
at concentration above 20 mM
ribavirin-TP
-
at ATP concentration equal to Km, IC50 of NTPase reaction is 0.4 mM, classical competitive inhibitor with regard to ATP. At ATP and DNA duplex concentrations corresponding to their KM-values an IC50 of 0.12 mM is measured. Inhibition reaches a maximum of 30% of the control at 0.45 mM and is not competitive with regard to ATP
RNA
homopolymeric RNA inhibits under otherwise optimal conditions
Sodium fluoride
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
-
stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 128% relative activity at a concentration of 0.05 mM
4,5-dihydro-8H-6-(N-phenyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dione
-
activates
acetyl-CoA
-
stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 127% relative activity at a concentration of 0.05 mM
choline
-
activates at concentration below 20 mM
D-fructose 1,6-bisphosphate
-
stimulating in physiological concentrations, 155% relative activity at a concentration of 0.5 mM, 126% relative activity at a concentration of 0.05 mM
O6-benzyl-N7-chloroethylguanine
-
weak inhibition of NTPase activity, enhances helicase activity up to 850% of control
O6-benzyl-N9-chloroethylguanine
-
stimulates NTPase activity with a maximum effect of 350% of control at 0.65 mM, enhances helicase activity up to 220% of control
Poly(A)
-
stimulates the ATP hydrolysis dependent on the chain length, highest activation by poly(A)40 comapred to shorter molecules
poly(dA)
-
1.7-3.3 mM, activation of ATPase activity to 170-180% of control
Poly(U)
-
the activation by poly(U) is inhibited by K+
Polynucleotide
-
denatured DNA and RNA, synthetic RNA and DNA hybrids
RNA
-
stimulates the ATP hydrolysis dependent on the chain length
taurine
-
activates the enzyme, and acts as antagonist and reduces the hypochlorous acid toxicity
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
2',3'-didehydro-thymidine triphosphate
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
2.3
3'-azido-2',3'-dideoxythymidine triphosphate
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
0.046 - 0.217
ADP
0.000028 - 2.5
ATP
0.082 - 0.5
CDP
0.006 - 3.8
CTP
0.145
dADP
substrate concentration between 0.01 and 4.0 mM, 37C
0.062 - 6.9
dATP
0.092
dCDP
substrate concentration between 0.01 and 4.0 mM, 37C
0.023 - 4
dCTP
3.8
ddCTP
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
0.9
ddTTP
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
0.051
dGDP
substrate concentration between 0.01 and 4.0 mM, 37C
0.083 - 0.144
dGTP
0.088
dITP
substrate concentration between 0.01 and 4.0 mM, 37C
0.0000000047
DNA duplex
-
-
-
0.04 - 2.4
dTTP
0.092
GDP
substrate concentration between 0.01 and 4.0 mM, 37C
0.004 - 3.7
GTP
0.046
IDP
substrate concentration between 0.01 and 4.0 mM, 37C
0.025 - 0.147
ITP
0.8
L-dATP
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
9.9
L-dCTP
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
28
L-dTTP
-
pH 7.5, 25C, NTP hydrolysis-dependent helicase activity
0.0185
MgATP2-
-
concentration of MgATP2- higher than 0.006 mM
0.00032
RNA
-
wild-type transcriptase cofactor my2, 22C, 6.5-7.0
0.105
TDP
substrate concentration between 0.01 and 4.0 mM, 37C
0.007 - 0.138
TTP
0.137
UDP
substrate concentration between 0.01 and 4.0 mM, 37C
0.005 - 9.4
UTP
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0051 - 0.29
ADP
0.0086 - 4384
ATP
1.67
CDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.0073 - 2286
CTP
0.52
dADP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.057 - 3150
dATP
1.12
dCDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.3 - 5096
dCTP
0.51
dGDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.4 - 2500
dGTP
0.22
dITP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
10.9
dTTP
Saccharomyces cerevisiae
-
pH 7.5, 23C
82 - 1875
dUTP
0.39
GDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.0076 - 2963
GTP
0.06
IDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.18
ITP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.005
RNA
mammalian orthoreovirus
-
wild-type transcriptase cofactor my2, 22C, 6.5-7.0
1.03
TDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.0074 - 0.69
TTP
1.35
UDP
Deinococcus radiodurans
Q9RXX6
substrate concentration between 0.01 and 4.0 mM, 37C
0.007 - 2162
UTP
additional information
additional information
West Nile virus
-
at optimum Mg2+ and saturating ATP concentrations 1 pmol of enzyme unwinds 5.5 fmol of DNA duplex per s
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
2',3'-didehydro-thymidine triphosphate
-
pH 7.5, 25C, inhibition of ATPase activity
2.5
2',3'-dideoxy-5-fluorocytidine triphosphate
-
pH 7.5, 25C, inhibition of ATPase activity
2.1
3'-azido-2',3'-dideoxythymidine triphosphate
-
pH 7.5, 25C, inhibition of ATPase activity
0.54
ATP
-
pH 7.5, 25C, inhibition of ATPase activity
0.6
dATP
-
pH 7.5, 25C, inhibition of ATPase activity
3
dCTP
-
pH 7.5, 25C, inhibition of ATPase activity
3.8
ddCTP
-
pH 7.5, 25C, inhibition of ATPase activity
1.2
ddTTP
-
pH 7.5, 25C, inhibition of ATPase activity
3.5
dGTP
-
pH 7.5, 25C, inhibition of ATPase activity
2.8
dTTP
-
pH 7.5, 25C, inhibition of ATPase activity
1.2
L-dATP
-
pH 7.5, 25C, inhibition of ATPase activity
1.5
L-dCTP
-
pH 7.5, 25C, inhibition of ATPase activity
2.3
L-ddCTP
-
pH 7.5, 25C, inhibition of ATPase activity
1.3
L-dTTP
-
pH 7.5, 25C, inhibition of ATPase activity
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075
5-fluoro-2-selenocytosine
West Nile virus
-
IC50: 0.075 mM for NTPase reaction, no influence to helicase activity up to a concentration of 0.5 mM
0.12
ribavirin-TP
West Nile virus
-
at ATP concentration equal to Km, IC50 of NTPase reaction is 0.4 mM, classical competitive inhibitor with regard to ATP. At ATP and DNA duplex concentrations corresponding to their KM-values an IC50 of 0.12 mM is measured. Inhibition reaches a maximum of
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
broad range optimum, pH profile
7.2 - 7.5
ATPase activity
7.4
-
ATPase assay at
8.5
-
assay at
9
hydrolysis of CDP
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
pH profile
5.7 - 9
-
50% of maximal activity at pH 5.7, and pH 9.0
6 - 8.5
-
pH 6.0: about 55% of maximal activity, pH 8.5: about 55% of maximal activity
6.8 - 8
pH 6.8: about 90% of maximal activity, pH 8.0: about 70% of maximal activity
9 - 9.5
optimal range for hydrolysis of CDP between
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
helicase assay at
60
-
ATPase assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 44
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
purified from
Manually annotated by BRENDA team
-
from virus-infected Vero cells
Manually annotated by BRENDA team
-
expression in
Manually annotated by BRENDA team
-
expression in
Manually annotated by BRENDA team
-
expression in
Manually annotated by BRENDA team
-
small mesenteric artery
Manually annotated by BRENDA team
-
expression in intermediary, primary, and secondary follicles
Manually annotated by BRENDA team
-
expression in
Manually annotated by BRENDA team
-
expression in
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
nuclear envelope
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20500
-
x * 20500, recombinant enzyme, SDS-PAGE
40000
-
SDS-PAGE
46000
-
SDS-PAGE
58600
-
gel filtration, at 4C in 20 mM Tris-HCl, 0.1 M NaCl, pH 7.5
60200
-
SDS-PAGE
75000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information