Literature summary for 3.6.1.15 extracted from

Matoba, K.; Shiba, T.; Takeuchi, T.; Sibley, L.D.; Seiki, M.; Kikyo, F.; Horiuchi, T.; Asai, T.; Harada, S.
Crystallization and preliminary X-ray structural analysis of nucleoside triphosphate hydrolases from Neospora caninum and Toxoplasma gondii (2010), Acta Crystallogr. Sect. F, 66, 1445-1448.

Search for more literature for 3.6.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Toxoplasma gondii
expressed in Escherichia coli BL21 cells	Neospora caninum

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 10-12% (w/v) PEG 3350, 0.1 M Tris-HCl pH 7.4, 2% (v/v) glycerol, at 20°C	Toxoplasma gondii
sitting drop vapor diffusion method, using 8-10% (w/v) PEG 3350, 0.1 M Tris-HCl pH 7.4, 50 mM diammonium phosphate and 2%(v/v) glycerol, at 20°C	Neospora caninum

Organism

Organism	UniProt	Comment	Textmining
Neospora caninum	C9K7J7	-	-
Toxoplasma gondii	Q27893	-	-

Purification (Commentary)

Purification (Comment)	Organism
Toyopearl DEAE-650S column chromatography, Source 15Q column chromatography, and Superdex 200 pg gel filtration	Toxoplasma gondii
Toyopearl DEAE-650S column chromatography, Source 15Q column chromatography, and Superdex 200 pg gel filtration	Neospora caninum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Toxoplasma gondii	ADP + phosphate	-	?
ATP + H2O	-	Neospora caninum	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
NTPase	-	Neospora caninum
NTPase-I	-	Toxoplasma gondii
nucleoside triphosphate hydrolase	-	Toxoplasma gondii
nucleoside triphosphate hydrolase	-	Neospora caninum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)