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Literature summary for 3.6.1.15 extracted from
- NMR structure and functional characterization of a human cancer-related nucleoside triphosphatase (2007), J. Mol. Biol., 367, 788-801.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E113T | site-directed mutagenesis, the mutation in the NTP binding site has a significant effect on the hydrolysis rate of the purine nucleotides, but has only a small effect on the protein structure | Homo sapiens |
| G115H | site-directed mutagenesis, the mutation in the NTP binding site has a significant effect on the hydrolysis rate of the purine nucleotides, but has only a small effect on the protein structure | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
GTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens |  |
| 0.005 | - |
ATP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.005 | - |
UTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.006 | - |
CTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.007 | - |
TTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.046 | - |
ADP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20500 | - |
x * 20500, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Homo sapiens | - |
AMP + phosphate | - |
? | |
| ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
| CTP + H2O | Homo sapiens | - |
CDP + phosphate | - |
? | |
| GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
| TTP + H2O | Homo sapiens | - |
TDP + phosphate | - |
? | |
| UTP + H2O | Homo sapiens | - |
UDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange chromatography and gel filtration to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Homo sapiens | AMP + phosphate | - |
? | |
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | HCR-NTPase contains both residues E113 and G115, and has nearly identical hydrolysis rates for ATP and GTP | Homo sapiens | ADP + phosphate | - |
? | |
| CTP + H2O | - |
Homo sapiens | CDP + phosphate | - |
? | |
| GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
| GTP + H2O | HCR-NTPase contains both residues E113 and G115, and has nearly identical hydrolysis rates for ATP and GTP | Homo sapiens | GDP + phosphate | - |
? | |
| additional information | HCR-NTPase is a non-selective NTPase with only slightly higher activity for purine nucleoside triphosphates, and with a comparatively low rate of hydrolysis, structural basis of HCR-NTPase activity, overview | Homo sapiens | ? | - |
? | |
| TTP + H2O | - |
Homo sapiens | TDP + phosphate | - |
? | |
| UTP + H2O | - |
Homo sapiens | UDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20500, recombinant enzyme, SDS-PAGE | Homo sapiens |
| More | three-dimensional structure determination by NMR and MALDI-TOF mass spectrometry, and structure analysis. The structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A, GPPGVGKT, and Walker B, VCVIDEIG, motifs, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HCR-NTPase | - |
Homo sapiens |
| human cancer-related nucleoside triphosphatase | - |
Homo sapiens |
| non-specific nucleoside triphosphatase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0051 | - |
ADP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.007 | - |
UTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.0073 | - |
CTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.0074 | - |
TTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.0076 | - |
GTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.0086 | - |
ATP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
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