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Literature summary for 3.6.1.15 extracted from
- Purification and characterization of West Nile virus nucleoside triphosphatase (NTPase)/helicase: evidence for dissociation of the NTPase and helicase activities of the enzyme (2001), J. Virol., 75, 3220-3229.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| O6-benzyl-N7-chloroethylguanine | weak inhibition of NTPase activity, enhances helicase activity up to 850% of control | West Nile virus |  |
| O6-benzyl-N9-chloroethylguanine | stimulates NTPase activity with a maximum effect of 350% of control at 0.65 mM, enhances helicase activity up to 220% of control | West Nile virus | |
| poly(dA) | 1.7-3.3 mM, activation of ATPase activity to 170-180% of control | West Nile virus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluoro-2-selenocytosine | IC50: 0.075 mM for NTPase reaction, no influence to helicase activity up to a concentration of 0.5 mM | West Nile virus | |
| ADP | - |
West Nile virus | |
| K+ | above 500 mM | West Nile virus | |
| Mg2+ | above 10 mM | West Nile virus | |
| Na+ | above 500 mM | West Nile virus | |
| O6-benzyl-N7-chloroethylguanine | weak inhibition of NTPase activity, enhances helicase activity | West Nile virus | |
| O6-benzylguanine | weak inhibitor of ATPase and helicase activity | West Nile virus | |
| oligo(dA)25 | above 0.5 mM, inhibits unwinding reaction | West Nile virus | |
| ribavirin-TP | at ATP concentration equal to Km, IC50 of NTPase reaction is 0.4 mM, classical competitive inhibitor with regard to ATP. At ATP and DNA duplex concentrations corresponding to their KM-values an IC50 of 0.12 mM is measured. Inhibition reaches a maximum of 30% of the control at 0.45 mM and is not competitive with regard to ATP | West Nile virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000000047 | - |
DNA duplex | - |
West Nile virus | |
| 0.0095 | - |
ATP | - |
West Nile virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 100-300 mM, activation of ATPase reaction to about 200% of control | West Nile virus | |
| Mg2+ | required. 150% of the control at an optimum Mg2+ concentration of 1-3 mM | West Nile virus | |
| Na+ | 100-300 mM, activation of ATPase reaction to about 200% of control | West Nile virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | West Nile virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| West Nile virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
West Nile virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | from virus-infected Vero cells | West Nile virus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160.8 | - |
- |
West Nile virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
West Nile virus | ADP + phosphate | - |
? | |
| DNA duplex + H2O | - |
West Nile virus | ? | - |
? | |
| additional information | helicase activity, the enzyme catalyzes the unwinding of DNA duplex | West Nile virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | West Nile virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nucleoside triphosphatase (NTPase)/helicase | - |
West Nile virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | at optimum Mg2+ and saturating ATP concentrations 1 pmol of enzyme unwinds 5.5 fmol of DNA duplex per s | West Nile virus | |
| 133 | - |
ATP | pH 7.5, 30°C | West Nile virus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
IC50: 0.075 mM for NTPase reaction, no influence to helicase activity up to a concentration of 0.5 mM | West Nile virus | 5-fluoro-2-selenocytosine | |
| 0.12 | - |
at ATP concentration equal to Km, IC50 of NTPase reaction is 0.4 mM, classical competitive inhibitor with regard to ATP. At ATP and DNA duplex concentrations corresponding to their KM-values an IC50 of 0.12 mM is measured. Inhibition reaches a maximum of | West Nile virus | ribavirin-TP | |
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