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Literature summary for 3.6.1.15 extracted from

  • Kim, J.; Parker, J.S.; Murray, K.E.; Nibert, M.L.
    Nucleoside and RNA triphosphatase activities of Orthoreovirus transcriptase cofactor mu2 (2004), J. Biol. Chem., 279, 4394-4403.
    View publication on PubMed
Search for more literature for 3.6.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene M1, expression of transcriptase cofactor my2 mutant K415A/K419A in Spodoptera frugiperda Sf21 insect cells using the baculovirus system mammalian orthoreovirus

Protein Variants

Protein Variants Comment Organism
K415A/K419A site-directed mutagenesis, mutation of residues in the nucleotide binding domain of transcriptase cofactor my2, leads to loss of enzyme activity mammalian orthoreovirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
 RNA wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
2.5
-
 ATP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.7
-
 GTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.8
-
 CTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
9.4
-
 UTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates mammalian orthoreovirus
Mg2+ activates mammalian orthoreovirus
Mn2+ activates mammalian orthoreovirus
additional information the enzyme activity depends on divalent cations and prefers Mg2+, Ca2+, and Mn2+ mammalian orthoreovirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O mammalian orthoreovirus removal of the gamma-phosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
mammalian orthoreovirus
-
 mORV
-
mammalian orthoreovirus mORV
-
 mORV
-

Purification (Commentary)

Purification (Comment) Organism
transcriptase cofactor my2 from host nucleoplasma by ion exchange and adsorption chromatography at room temperature mammalian orthoreovirus

Source Tissue

Source Tissue Comment Organism Textmining
additional information the transcriptase cofactor my2 is associated to microtubules in host cells, overview mammalian orthoreovirus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 mammalian orthoreovirus ADP + phosphate
-
 ?
ATP + H2O
-
 mammalian orthoreovirus mORV ADP + phosphate
-
 ?
CTP + H2O
-
 mammalian orthoreovirus CDP + phosphate
-
 ?
CTP + H2O
-
 mammalian orthoreovirus mORV CDP + phosphate
-
 ?
GTP + H2O
-
 mammalian orthoreovirus GDP + phosphate
-
 ?
GTP + H2O
-
 mammalian orthoreovirus mORV GDP + phosphate
-
 ?
additional information the virus core particles, an icosahedral multienzyme complex, and the transcriptase cofactor my2 show nucleoside triphosphatase activity mammalian orthoreovirus ?
-
 ?
additional information the virus core particles, an icosahedral multienzyme complex, and the transcriptase cofactor my2 show nucleoside triphosphatase activity mammalian orthoreovirus mORV ?
-
 ?
RNA + H2O removal of the gamma-phosphate mammalian orthoreovirus ?
-
 ?
UTP + H2O
-
 mammalian orthoreovirus UDP + phosphate
-
 ?
UTP + H2O
-
 mammalian orthoreovirus mORV UDP + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature mammalian orthoreovirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 RNA wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
7.3
-
 UTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
8
-
 CTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
9.8
-
 GTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
13.8
-
 ATP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 mammalian orthoreovirus

pH Range

pH Minimum pH Maximum Comment Organism
5.7 9 50% of maximal activity at pH 5.7, and pH 9.0 mammalian orthoreovirus