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Literature summary for 3.6.1.15 extracted from

  • Benarroch, D.; Selisko, B.; Locatelli Giada, A.; Maga, G.; Romette, J.L.; Canard, B.
    The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core (2004), Virology, 328, 208-218.
    View publication on PubMed
Search for more literature for 3.6.1.15

Activating Compound

Activating Compound Comment Organism Structure
DTT
-
 Dengue virus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Dengue virus

Protein Variants

Protein Variants Comment Organism
D284A site-directed mutagenesis, mutation in the Walker B motif, inactive mutant Dengue virus
E285A site-directed mutagenesis, mutation in the Walker B motif, the mutant does not show RTPase and NTPase activities, and is unable to remove gamma-phosphate from substrate pppAC5 Dengue virus
E578A site-directed mutagenesis, mutation of a highly conserved residue, the mutant retains full NTPase, RTPase and helicase activities Dengue virus
K199A site-directed mutagenesis, mutation in the Walker B motif, the mutant does not show RTPase and NTPase activities, and is unable to remove gamma-phosphate from substrate pppAC5 Dengue virus
additional information construction of mutant DELTANS3 comprising the C-terminal 54-kDa domain of NS3, amino acid residues 169-619 Dengue virus
R513A site-directed mutagenesis, mutation in the C-terminal domain of DELTANS3, the mutation reduces NTPase activity, abrogates helicase activity and strongly diminishes RTPase activity Dengue virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Dengue virus
0.108
-
 ATP recombinant wild-type enzyme, pH 7.4, 30°C Dengue virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strictly dependent on, coordination of Mg2+ by the Walker B motif, required for all three activities, the RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activity Dengue virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Dengue virus ATPase activity ADP + phosphate
-
 ?
additional information Dengue virus the enzyme is involved in RNA replication and capping, overview ?
-
 ?
NTP + H2O Dengue virus NTPase activity NDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Dengue virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinat His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Dengue virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of wild-type andmutant enzymes, overview Dengue virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O ATPase activity Dengue virus ADP + phosphate
-
 ?
additional information the enzyme is involved in RNA replication and capping, overview Dengue virus ?
-
 ?
additional information the multifunctional enzyme shows RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities, which are located on the C-terminal 54-kDa domain, overview Dengue virus ?
-
 ?
NTP + H2O NTPase activity Dengue virus NDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
More RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities are located on the C-terminal 54-kDa domain, the RTPase activity requuires an intact Walker B motif in the helicase core Dengue virus

Synonyms

Synonyms Comment Organism
NS3
-
 Dengue virus
nucleotide 5'-triphosphatase
-
 Dengue virus
protein NS3
-
 Dengue virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 helicase assay at Dengue virus
30
-
 ATPase assay at Dengue virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 ATPase assay at Dengue virus
7.5
-
 helicase assay at Dengue virus