Information on EC 3.5.2.9 - 5-oxoprolinase (ATP-hydrolysing)

3.5.2.9

-

5-oxoprolinase (ATP-hydrolysing)

GO:0017168

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate

-

-

-

-

carboxylic acid amide hydrolysis

-

-

-

-

hydrolysis

Arabidopsis thaliana

Q9FIZ7

-

689627

gamma-glutamyl cycle

-

PWY-4041

Glutathione metabolism

00480

-

5-oxo-L-proline amidohydrolase (ATP-hydrolysing)

-

5-OPase

-

-

-

-

5-oxo-L-prolinase

-

-

-

-

5-oxo-L-prolinase

Rattus norvegicus

-

-

694485

5-oxoprolinase

-

-

-

-

5-oxoprolinase

Arabidopsis thaliana

Q9FIZ7

-

689627

5OPase

Arabidopsis thaliana

Q9FIZ7

-

689627

L-pyroglutamate hydrolase

-

-

-

-

OPase

Rattus norvegicus

-

-

694485

oxoprolinase

-

-

-

-

OXP1

Saccharomyces cerevisiae

P28273

-

712045

pyroglutamase

-

-

-

-

pyroglutamase (ATP-hydrolysing)

-

-

-

-

pyroglutamate hydrolase

-

-

-

-

pyroglutamic hydrolase

-

-

-

-

9075-46-1

-

Alcaligenes sp.

strain F-137

209394

-

-

Alcaligenes sp. F-137

strain F-137

209394

-

-

Arabidopsis thaliana

wild-type plants and oxp1-1 and oxp2-2 mutants

689627

Q9FIZ7

UniProt

Bos taurus

-

667886

Q75WB5

Uniprot

Bos taurus

cow

209381

-

-

Brassica oleracea

cauliflower

209379

-

-

Brassica rapa

turnip

209379

-

-

Canis lupus familiaris

dog

209381

-

-

Cucurbita pepo

squash

209379

-

-

Daucus carota

carrot

209379

-

-

Felis catus

cat

209381

-

-

Homo sapiens

-

688174, 704751

-

-

Homo sapiens

breast cancer cell line MCF7; human

209391

-

-

Homo sapiens

human

209384, 209386, 209387, 209389, 209390

-

-

Mus musculus

-

209374, 209381, 209382, 209384, 209385, 209386

-

-

Mus musculus

mouse, NCS strain

209370

-

-

Mus musculus NCS

mouse, NCS strain

209370

-

-

Oryctolagus cuniculus

rabbit

209381

-

-

Ovis aries

sheep

209381, 209387

-

-

Paecilomyces variotii

strain F-1

209394

-

-

Paecilomyces variotii F-1

strain F-1

209394

-

-

Pisum sativum

pea

209379

-

-

Pseudomonas putida

-

209373, 209388, 209394

-

-

Pseudomonas putida

strain ALA

209371

-

-

Pseudomonas putida ALA

strain ALA

209371

-

-

Pseudomonas sp.

-

209379, 209384, 209385

-

-

Raphanus sativus

radish

209379

-

-

Rattus norvegicus

rat

209370, 209371, 209372, 209373, 209374, 209375, 209376, 209377, 209379, 209380, 209382, 209383, 209384, 209385, 209387, 209390, 209393, 209394

-

-

Rattus norvegicus

rat

209388

P97608

Uniprot

Rattus norvegicus

rat; strain Sprague-Dawley

209381, 209386

-

-

Rattus norvegicus

rat; strain Wistar albino

209378

-

-

Rattus norvegicus

Sprague-Dawley rats, female

694485

-

-

Rattus norvegicus Sprague-Dawley

strain Sprague-Dawley

209381, 209386

-

-

Rattus norvegicus Wistar albino

strain Wistar albino

209378

-

-

Saccharomyces cerevisiae

-

712045

P28273

UniProt

Solanum tuberosum

potato

209379

-

-

Spinacia oleracea

spinach

209379

-

-

Sus scrofa

pig

209381, 209387, 209392, 209393

-

-

Triticum aestivum

wheat

209379

-

-

physiological function

Saccharomyces cerevisiae

P28273, -

comparison of the growth of 5-oxoprolinase deletion strains with wild-type strains in a variety of growth conditions does not yield any discernible phenotypes. Cells of glutamate auxotroph aco1D strain expressing OXP1 are able to grow on 5-oxoproline, although the growth is very slow

712045

2-imidazolidone-4-carboxylate + ATP + H2O

2-imidazolidone + ADP + phosphate

Rattus norvegicus

-

-

209381

-

-

?

2-imidazolidone-4-carboxylate + ATP + H2O

2-imidazolidone + ADP + phosphate

Rattus norvegicus

-

promotes enzymatic hydrolysis of ATP but is not itself hydrolyzed

209375, 209376, 209377

-

-

?

2-imidazoline-4-carboxylate + ATP + H2O

2-imidazoline-4-carboxylate + ADP + phosphate

Rattus norvegicus

-

does not undergo ring cleavage, but stimulates formation of inorganic phosphate from ATP

209374

-

-

?

2-imidazolinone-4-carboxylate + H2O

?

Rattus norvegicus

-

-

209374

-

-

?

2-oxo-5,5-dimethylthiazolidine-4-carboxylate + H2O

?

Rattus norvegicus

-

-

209374

-

-

?

2-piperidone-6-carboxylate + ATP + H2O

2-aminoadipate + phosphate + ADP

Rattus norvegicus

-

-

209374, 209376, 209377

-

-

?

2-piperidone-6-carboxylate + ATP + H2O

2-aminoadipate + phosphate + ADP

Rattus norvegicus

-

-

209381

-

209381

?

2-thiazolinone-4-carboxylate + H2O

?

Rattus norvegicus

-

-

209374

-

-

?

3-methyl-5-oxoproline + H2O

2-amino-3-methylpentanedioic acid

Rattus norvegicus

-

-

209376

-

-

?

3-oxy-5-oxoproline + H2O

3-oxyglutamate + phosphate

Rattus norvegicus

-

-

209374

-

-

?

3-oxy-5-oxoproline + H2O

3-oxyglutamate + phosphate

Rattus norvegicus

-

-

209381

-

209381

?

4-methyl-5-oxoproline + H2O

2-amino-4-methylpentanedioic acid

Rattus norvegicus

-

-

209376

-

-

?

4-oxy-5-oxoproline + H2O

4-oxyglutamate + phosphate

Rattus norvegicus

-

-

209374

-

-

?

4-oxy-5-oxoproline + H2O

4-oxyglutamate + phosphate

Rattus norvegicus

-

-

209381

-

209381

?

5'-p-fluorosulfonylbenzoyladenosine + H2O

?

Rattus norvegicus

-

-

209375

-

-

?

5'-p-fluorosulfonylbenzoylinosine + H2O

?

Rattus norvegicus

-

-

209375

-

-

?

5-oxo-L-proline + ATP + 2 H2O

L-glutamate + phosphate + ADP

Rattus norvegicus

-

assay at pH 7.2, 5 mM substrate, 4°C, 20 min

694485

-

-

?

5-oxo-L-proline + ATP + H2O

L-glutamate + ADP + phosphate

Arabidopsis thaliana

Q9FIZ7

assay at 30°C, pH 9.5, reaction stopped by 20 microl acetic acid and heating for 5 min at 100°C

689627

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Mus musculus

-

-

209370, 209374, 209381, 209382, 209384, 209385, 209386

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Homo sapiens

-

-

209384, 209386, 209387, 209389, 209390, 209391

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

-

209370, 209371, 209373, 209374, 209375, 209376, 209377, 209378, 209379, 209380, 209381, 209382, 209383, 209384, 209385, 209386, 209387

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

P97608

-

209388

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

-

209390, 209393, 209394

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

-

209372

-

-

ir

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Sus scrofa

-

-

209381, 209387, 209392, 209393

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Bos taurus

-

-

209381, 667886

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Bos taurus

Q75WB5

-

667886

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Triticum aestivum

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Oryctolagus cuniculus, Ovis aries

-

-

209381

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Ovis aries

-

-

209387

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Spinacia oleracea, Pisum sativum, Solanum tuberosum

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Paecilomyces variotii

-

-

209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Pseudomonas sp.

-

-

209379, 209384, 209385

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Pseudomonas putida

-

-

209373, 209388, 209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Canis lupus familiaris, Felis catus

-

-

209381

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Daucus carota, Raphanus sativus

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Alcaligenes sp.

-

-

209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Brassica oleracea, Cucurbita pepo, Brassica rapa

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Pseudomonas putida

-

component F8 + component beta5

209371

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations

209377

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations

209372

-

-

ir

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Saccharomyces cerevisiae

P28273, -

ATP is required

712045

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus Sprague-Dawley

-

-

209386

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Paecilomyces variotii F-1, Alcaligenes sp. F-137

-

-

209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Mus musculus NCS

-

-

209370

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus Wistar albino

-

-

209378

-

-

-

alpha-hydroxyglutarate lactone + H2O

?

Rattus norvegicus

-

-

209372, 209377

-

-

?

ATP + H2O

?

Rattus norvegicus

-

-

209372, 209375, 209376, 209379, 209380, 209381

-

-

?

ATP + H2O

?

Triticum aestivum

-

-

209379

-

-

?

ATP + H2O

?

Rattus norvegicus Sprague-Dawley

-

-

209381

-

-

?

CTP + H2O

?

Pseudomonas putida, Pseudomonas putida ALA

-

less than 5% of the activity observed with ATP

209371

-

-

?

dATP + H2O

?

Rattus norvegicus

-

-

209371, 209380, 209381

-

-

?

dATP + H2O

?

Pseudomonas putida

-

-

209371

-

-

?

dATP + H2O

?

Rattus norvegicus Sprague-Dawley

-

-

209381

-

-

?

dATP + H2O

?

Pseudomonas putida ALA

-

-

209371

-

-

?

DL-2-oxo-3,3-dimethylthiazolidine-4-carboxylate + H2O

?

Rattus norvegicus

-

-

209374

-

-

?

DL-cis-2-oxo-5-methyloxazolidine-4-carboxylate + H2O

DL-threonine + ADP + phosphate

Rattus norvegicus

-

-

209374

-

-

?

GTP + H2O

?

Rattus norvegicus

-

-

209371, 209375, 209376, 209379, 209380, 209381

-

-

?

GTP + H2O

?

Triticum aestivum

-

-

209379

-

-

?

GTP + H2O

?

Pseudomonas putida

-

less than 5% of the activity observed with ATP

209371

-

-

?

GTP + H2O

?

Rattus norvegicus Sprague-Dawley

-

-

209381

-

-

?

GTP + H2O

?

Pseudomonas putida ALA

-

less than 5% of the activity observed with ATP

209371

-

-

?

ITP + H2O

?

Rattus norvegicus

-

-

209372, 209375, 209376, 209379, 209380, 209381

-

-

?

ITP + H2O

?

Triticum aestivum

-

-

209379

-

-

?

ITP + H2O

?

Rattus norvegicus Sprague-Dawley

-

-

209381

-

-

?

L-2-iminothiazolidine-4-carboxylate + H2O

L-cystine

Rattus norvegicus

-

-

209374

-

-

?

L-2-oxooxazolidine-4-carboxylate + H2O + ATP

L-serine + ADP + phosphate

Rattus norvegicus

-

-

209374

-

-

?

L-2-oxothiazolidine-4-carboxylate + H2O + ATP

L-cysteine + ADP + phosphate

Homo sapiens

-

-

209389, 209390

-

-

?

L-2-oxothiazolidine-4-carboxylate + H2O + ATP

L-cysteine + ADP + phosphate

Rattus norvegicus

-

-

209370, 209371, 209372, 209373, 209390, 209394

-

-

?

L-2-oxothiazolidine-4-carboxylate + H2O + ATP

L-cysteine + ADP + phosphate

Paecilomyces variotii

-

-

209394

-

-

?

L-2-oxothiazolidine-4-carboxylate + H2O + ATP

L-cysteine + ADP + phosphate

Pseudomonas putida

-

-

209371, 209373, 209394

-

-

?

L-2-oxothiazolidine-4-carboxylate + H2O + ATP

L-cysteine + ADP + phosphate

Alcaligenes sp., Paecilomyces variotii F-1, Alcaligenes sp. F-137

-

-

209394

-

-

?

L-dihydroorotate + H2O

?

Rattus norvegicus

-

do not undergo ring cleavage, but stimulates formation of inorganic phosphate from ATP

209374

-

-

?

L-trans-2-oxo-5-methyloxazolidine-4-carboxylate + H2O

L-threonine + ADP + phosphate

Rattus norvegicus

-

-

209374

-

-

?

MgATP2- + H2O

?

Rattus norvegicus

-

-

209382, 209387

-

-

-

MgATP2- + H2O

?

Rattus norvegicus

-

-

209371, 209383

-

-

?

MgATP2- + H2O

?

Triticum aestivum

-

-

209379

-

-

-

MgATP2- + H2O

?

Pseudomonas putida

-

-

209373

-

-

-

UTP + H2O

?

Rattus norvegicus

-

-

209371, 209375, 209376, 209380, 209381

-

-

?

UTP + H2O

?

Pseudomonas putida, Pseudomonas putida ALA

-

less than 5% of the activity observed with ATP

209371

-

-

?

XTP + H2O

?

Pseudomonas putida

-

less than 5% of the activity observed with ATP

209371

-

-

?

MgATP2- + H2O

?

Pseudomonas putida

-

component beta5

209371

-

-

?

additional information

?

-

Pseudomonas putida

-

-

209394

-

-

-

additional information

?

-

Rattus norvegicus

-

L-thioproline is no substrate

209374

-

-

-

additional information

?

-

Rattus norvegicus

-

5-oxo-D-proline, 2-pyrrolidone-4-carboxylate, N-methyl-5-oxo-L-proline and N-acetyl-L-alanine are no substrates

209376

-

-

-

additional information

?

-

Rattus norvegicus

-

L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates

209381

-

-

-

additional information

?

-

Paecilomyces variotii

-

CTP, TTP and UTP are no substrates

209394

-

-

-

additional information

?

-

Triticum aestivum

-

CTP and UTP are poor substrates

209379

-

-

-

additional information

?

-

Pseudomonas putida

-

no activity observed with 5-oxo-D-proline

209371, 209373

-

-

-

additional information

?

-

Rattus norvegicus Sprague-Dawley

-

L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates

209381

-

-

-

additional information

?

-

Paecilomyces variotii F-1

-

CTP, TTP and UTP are no substrates

209394

-

-

-

additional information

?

-

Pseudomonas putida ALA

-

no activity observed with 5-oxo-D-proline

209371

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Mus musculus

-

-

209370, 209381, 209382, 209384, 209385, 209386

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Homo sapiens

-

-

209384, 209386, 209387, 209389, 209390, 209391

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

-

209370, 209373, 209375, 209376, 209378, 209379, 209380, 209381, 209382, 209383, 209384, 209385, 209386, 209387

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

P97608

-

209388

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

-

209390, 209393, 209394

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Sus scrofa

-

-

209381, 209387, 209392, 209393

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Bos taurus

-

-

209381

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Triticum aestivum

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Oryctolagus cuniculus, Ovis aries

-

-

209381

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Ovis aries

-

-

209387

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Spinacia oleracea, Pisum sativum, Solanum tuberosum

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Paecilomyces variotii

-

-

209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Pseudomonas sp.

-

-

209379, 209384, 209385

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Pseudomonas putida

-

-

209373, 209388, 209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Canis lupus familiaris, Felis catus

-

-

209381

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Daucus carota, Raphanus sativus

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Alcaligenes sp.

-

-

209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Brassica oleracea, Cucurbita pepo, Brassica rapa

-

-

209379

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations

209377

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus

-

conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations

209372

-

-

ir

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus Sprague-Dawley

-

-

209386

-

-

-

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Paecilomyces variotii F-1, Alcaligenes sp. F-137

-

-

209394

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Mus musculus NCS

-

-

209370

-

-

?

5-oxo-L-proline + H2O + ATP

L-glutamate + ADP + phosphate

Rattus norvegicus Wistar albino

-

-

209378

-

-

-

Ca2+

Rattus norvegicus

-

promotes activity significantly

209375

Co2+

Rattus norvegicus

-

promotes activity significantly

209375

FeSO4

Paecilomyces variotii

-

1 mM , relative activity 113%

209394

K+

Pseudomonas putida

-

maximal activity of component A as an ATPase requires 80-300 mM

209373

K+

Triticum aestivum

-

requires monovalent cation, K+ or NH4+

209379

K+

Pseudomonas sp.

-

requires monovalent cation, K+ or NH4+

209379, 209385

K+

Rattus norvegicus

-

requires monovalent cation, K+ or NH4+

209379, 209381, 209385, 209387

K+

Paecilomyces variotii, Pseudomonas putida

-

-

209394

K+

Rattus norvegicus

-

-

209373, 209394

Mg2+

Triticum aestivum

-

-

209379

Mg2+

Paecilomyces variotii

-

-

209394

Mg2+

Rattus norvegicus

-

-

209375, 209379, 209381, 209387, 209394

Mn2+

Triticum aestivum

-

-

209379

Mn2+

Paecilomyces variotii

-

1 mM MnCl2, relative activity 144%

209394

Rb+

Triticum aestivum

-

-

209379

Mn2+

Rattus norvegicus

-

-

209379, 209381, 209387, 209394

additional information

Saccharomyces cerevisiae

P28273, -

enzyme requires ATP, a monovalent cation such as K+ or NH4+ and a divalent cation such as Mg2+ or Mn2+

712045

3-methyl-5-oxo-L-proline

Mus musculus

-

-

209370

2D-image

5-oxo-L-proline

Rattus norvegicus

-

inhibition of IPTase activity

209374, 209380

2D-image

AgNO3

Paecilomyces variotii

-

-

209394

2D-image

CuSO4

Paecilomyces variotii

-

-

209394

2D-image

DL-cis-2-oxo-5-methyloxazolidine-4-carboxylate

Rattus norvegicus

-

inhibition of IPTase activity

209374

2D-image

DTNB

Rattus norvegicus

-

could be reactivated with dithiothreitol

209375

2D-image

imidazolone carboxylate

Rattus norvegicus

-

-

209382

2D-image

iodoacetamide

Triticum aestivum

-

-

209379

2D-image

iodoacetamide

Rattus norvegicus

-

-

209379, 209381

2D-image

L-2-imidazolidone-4-carboxylate

Triticum aestivum

-

-

209379

2D-image

L-2-imidazolidone-4-carboxylate

Mus musculus

-

-

209370, 209374, 209381, 209384, 209386

2D-image

L-2-imidazolidone-4-carboxylate

Rattus norvegicus

-

-

209370, 209374, 209379, 209381, 209384, 209386

2D-image

L-2-iminothiazolidine-4-carboxylate

Rattus norvegicus

-

inhibition of IPTase activity

209374

2D-image

L-2-oxooxazolidine-4-carboxylate

Rattus norvegicus

-

inhibition of IPTase activity

209374

2D-image

L-2-Oxothiazolidine-4-carboxylate

Mus musculus

-

competitive inhibitor

209370

2D-image

L-2-Oxothiazolidine-4-carboxylate

Rattus norvegicus

-

-

209370

2D-image

L-2-Oxothiazolidine-4-carboxylate

Mus musculus

-

-

209374

2D-image

L-2-Oxothiazolidine-4-carboxylate

Rattus norvegicus

-

inhibition of IPTase activity

209374

2D-image

L-2-Oxothiazolidine-4-carboxylate

Homo sapiens

-

competitive inhibitor

209391

2D-image

L-dihydroorotic acid

Mus musculus, Rattus norvegicus

-

-

209381, 209384

2D-image

L-glutamine

Rattus norvegicus

-

inhibition of enzyme activity in tumor tissue

694485

2D-image

L-trans-2-oxo-5-methyloxazolidine-4-carboxylate

Rattus norvegicus

-

inhibition of glutamate formation; inhibition of IPTase activity

209374

-

N-bromosuccinimide

Paecilomyces variotii

-

-

209394

2D-image

N-ethylmaleimide

Triticum aestivum

-

-

209379

2D-image

N-ethylmaleimide

Paecilomyces variotii

-

-

209394

2D-image

N-ethylmaleimide

Rattus norvegicus

-

-

209375, 209379, 209381, 209394

2D-image

p-hydroxymercuribenzoate

Paecilomyces variotii

-

-

209394

2D-image

p-hydroxymercuribenzoate

Rattus norvegicus

-

-

209381, 209394

2D-image

ZnCl2

Paecilomyces variotii

-

-

209394

2D-image

2-imidazolidone-4-carboxylate

Rattus norvegicus

-

5-oxoproline analog, stimulates rapid ATP cleavage, but is not hydrolyzed itself

209372

2D-image

2-piperidone-6-carboxylate

Rattus norvegicus

-

stimulates ATP cleavage

209372

2D-image

L-glutamine

Rattus norvegicus

-

enhanced enzyme activity in jejunal mucosa of tumor-bearing rats

694485

2D-image

NH2OH-HCl

Paecilomyces variotii

-

1 mM, relative activity 109%

209394

2D-image

0.005

-

5-oxo-L-proline

Rattus norvegicus

-

-

209394

2D-image

0.008

-

5-oxo-L-proline

Pseudomonas putida, Rattus norvegicus

-

-

209371

2D-image

0.008

-

5-oxo-L-proline

Rattus norvegicus

-

-

209372, 209375, 209393

2D-image

0.009

-

5-oxo-L-proline

Rattus norvegicus

-

treated with N-ethylmaleimide

209375

2D-image

0.014

-

5-oxo-L-proline

Triticum aestivum

-

-

209379

2D-image

0.043

-

5-oxo-L-proline

Pseudomonas putida

-

-

209371, 209373, 209394

2D-image

0.0511

-

5-oxo-L-proline

Sus scrofa

-

-

209393

2D-image

0.0316

-

5-oxoproline

Rattus norvegicus

-

-

209382

2D-image

0.05

-

5-oxoproline

Rattus norvegicus

-

-

209381, 209385, 209393

2D-image

0.07

-

5-oxoproline

Alcaligenes sp.

-

-

209394

2D-image

0.14

-

5-oxoproline

Pseudomonas sp.

-

-

209379, 209385

2D-image

0.16

-

5-oxoproline

Rattus norvegicus

-

initial Km in presence of GTP

209376

2D-image

0.18

-

5-oxoproline

Rattus norvegicus

-

initial Km in presence of ITP

209376

2D-image

0.2

-

5-oxoproline

Rattus norvegicus

-

initial Km in presence of UTP

209376

2D-image

0.41

-

5-oxoproline

Rattus norvegicus

-

in presence of ATP

209376

2D-image

0.5

-

5-oxoproline

Rattus norvegicus

-

final Km in presence of UTP

209376

2D-image

0.5

-

5-oxoproline

Rattus norvegicus

-

-

209379

2D-image

0.57

-

5-oxoproline

Rattus norvegicus

-

final Km in presence of GTP

209376

2D-image

0.58

-

5-oxoproline

Rattus norvegicus

-

final Km in presence of ITP

209376

2D-image

0.03

-

ATP

Rattus norvegicus

-

without 5-oxoproline

209376

2D-image

0.091

-

ATP

Paecilomyces variotii

-

5-oxo-L-proline as substrate

209394

2D-image

0.13

-

ATP

Rattus norvegicus

-

measured in absence of 5-oxoproline

209372

2D-image

0.17

-

ATP

Rattus norvegicus

-

-

209372, 209375, 209379

2D-image

0.17

-

ATP

Triticum aestivum

-

-

209379

2D-image

0.17

-

ATP

Rattus norvegicus

-

-

209381

2D-image

0.21

-

ATP

Paecilomyces variotii

-

L-2-oxothiazolidine-4-carboxylate as substrate

209394

2D-image

0.4

-

ATP

Triticum aestivum

-

-

209379

2D-image

0.42

-

ATP

Rattus norvegicus

-

5-oxoproline added

209376

2D-image

1

-

ATP

Pseudomonas sp.

-

-

209379

2D-image

0.13

-

ITP

Rattus norvegicus

-

-

209372

2D-image

0.13

-

ITP

Rattus norvegicus

-

measured in absence of 5-oxoproline

209375

2D-image

0.17

-

ITP

Rattus norvegicus

-

without 5-oxoproline

209376

2D-image

0.41

-

ITP

Rattus norvegicus

-

5-oxoproline added

209376

2D-image

0.17

-

L-2-Oxothiazolidine-4-carboxylate

Paecilomyces variotii

-

-

209394

2D-image

0.002

-

L-2-oxothiazolidine-4-carboxylic acid

Pseudomonas putida, Rattus norvegicus

-

-

209371

2D-image

0.002

-

L-2-oxothiazolidine-4-carboxylic acid

Pseudomonas putida, Rattus norvegicus

-

-

209394

2D-image

0.1

-

MgATP2-

Rattus norvegicus

-

-

209387

2D-image

0.15

-

MgATP2-

Pseudomonas putida

-

-

209371, 209373

2D-image

0.1727

-

MgATP2-

Rattus norvegicus

-

-

209382

2D-image

4.2

-

MgATP2-

Triticum aestivum

-

-

209379

2D-image

0.5

-

MnATP2-

Triticum aestivum

-

-

209379

2D-image

0.17

-

UTP

Rattus norvegicus

-

without 5-oxoproline

209376

2D-image

1.11

-

UTP

Rattus norvegicus

-

5-oxoproline added

209376

2D-image

0.117

-

5-oxo-L-proline

Rattus norvegicus

-

-

209382

2D-image

0.00195

-

Bos taurus

-, Q75WB5

cytosolic fraction of kidney

667886

0.122

-

Rattus norvegicus

-

-

209382, 209383

0.625

-

Bos taurus

-, Q75WB5

after purification by Sepharyl S-300, Blue Sepharose and Phenyl Sepharose column chromatography

667886

0.69

-

Paecilomyces variotii

-

-

209394

0.88

-

Rattus norvegicus

-

-

209381

1

-

Rattus norvegicus

-

measured in tumor tissue after treatment with dietary glutamine

694485

1.25

-

Rattus norvegicus

-

measured in jejunal mucosa in DMBA-teated rats with tumor

694485

2.5

-

Rattus norvegicus

-

measured in tumor tissue

694485

2.91

-

Rattus norvegicus

-

measured in jejunal mucosa in DMBA-treated rats without tumor

694485

3

-

Rattus norvegicus

-

measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats without tumor

694485

3.4

-

Pseudomonas sp.

-

-

209385

3.6

-

Sus scrofa

-

-

209393

3.75

-

Rattus norvegicus

-

measured in jejunal mucosa in normal rats

694485

4.17

-

Rattus norvegicus

-

measured in jejunal mucosa after treatment with dietary glutamine in normal rats

694485

4.33

-

Rattus norvegicus

-

measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats with tumor

694485

7

-

Rattus norvegicus

-

-

209375

20.1

-

Triticum aestivum

-

-

209379

50

-

Rattus norvegicus

-

-

209380

75

-

Pseudomonas putida

-

component F8

209371

75

-

Pseudomonas putida

-

purified component B

209373

87

-

Rattus norvegicus

-

-

209372, 209375

243

-

Pseudomonas putida

-

component beta5

209371

243

-

Pseudomonas putida

-

purified component A

209373

7

8

Paecilomyces variotii

-

-

209394

7

-

Rattus norvegicus

-

second pH optimum at pH 9.7

209381

7.2

-

Rattus norvegicus

-

assay at

694485

7.8

8

Pseudomonas sp., Rattus norvegicus

-

Tris-HCl buffer

209385

7.8

-

Rattus norvegicus

-

-

209387

8.2

-

Pseudomonas putida

-

component beta5, cleavage of ATP

209371

8.2

-

Pseudomonas putida

-

component A-catalyzed 5-oxoproline-dependent cleavage of ATP

209373

9.5

-

Pseudomonas putida

-

component F8, ATP-dependent conversion of 5-oxoproline to glutamate

209371

9.5

-

Pseudomonas putida

-

component B

209373

9.5

-

Triticum aestivum

-

-

209379

9.5

-

Arabidopsis thaliana

Q9FIZ7

assay at

689627

9.7

-

Rattus norvegicus

-

second pH optimum at pH 7.0

209381

5.4

11.3

Rattus norvegicus

-

-

209380

5.5

11.2

Rattus norvegicus

-

-

209381

4

-

Rattus norvegicus

-

assay at

694485

30

-

Arabidopsis thaliana

Q9FIZ7

assay at

689627

50

-

Paecilomyces variotii

-

-

209394

30

55

Paecilomyces variotii

-

-

209394

blood

Homo sapiens

-

peripheral mononuclear cells

209390, 209391

brain

Oryctolagus cuniculus

-

-

209381

brain

Mus musculus

-

-

209381, 209384

brain

Homo sapiens

-

-

209384, 209387

brain

Rattus norvegicus

-

-

209381, 209384, 209387, 209388, 209393

brain

Sus scrofa

-

-

209381, 209387, 209392, 209393

brain

Rattus norvegicus Sprague-Dawley

-

-

-

breast

Homo sapiens

-

normal and cancer tissues

209389, 209391

breast cancer cell

Rattus norvegicus

-

-

694485

bud

Brassica oleracea

-

-

209379

cardiac muscle

Rattus norvegicus

-

-

209387, 209388, 209393

cardiac muscle

Sus scrofa

-

-

209387, 209393

choroid plexus

Mus musculus, Rattus norvegicus

-

-

209382, 209384

ciliary body

Oryctolagus cuniculus

-

-

209381

ciliary body

Rattus norvegicus

-

-

209381, 209382

colon

Homo sapiens

-

normal and cancer tissues

209389

eye

Mus musculus

-

-

209384

fibroblast

Homo sapiens

-

-

209384

fruit

Cucurbita pepo

-

-

209379

germ

Triticum aestivum

-

-

209379

intestine

Rattus norvegicus

-

-

209387

jejunal mucosa

Rattus norvegicus

-

-

694485

kidney

Canis lupus familiaris, Felis catus, Oryctolagus cuniculus

-

-

209381

kidney

Mus musculus

-

-

209370, 209374, 209381, 209382, 209384, 209385, 209386

kidney

Ovis aries

-

-

209381, 209387

kidney

Homo sapiens

-

-

209384, 209386, 209387, 209390

kidney

Rattus norvegicus

-

-

209370, 209371, 209372, 209373, 209374, 209375, 209377, 209378, 209379, 209380, 209381, 209382, 209383, 209384, 209385, 209386, 209387, 209388, 209390, 209393

kidney

Sus scrofa

-

-

209381, 209387, 209392, 209393

kidney

Bos taurus

-

-

209381, 667886

kidney

Mus musculus NCS

-

-

-

kidney

Rattus norvegicus Sprague-Dawley

-

;

-

kidney

Rattus norvegicus Wistar albino

-

-

-

leaf

Brassica rapa, Spinacia oleracea

-

-

209379

leaf

Arabidopsis thaliana

Q9FIZ7

-

689627

liver

Oryctolagus cuniculus

-

-

209381

liver

Mus musculus

-

-

209381, 209384

liver

Homo sapiens

-

-

209384, 209387, 209390

liver

Rattus norvegicus

-

-

209381, 209384, 209387, 209388, 209390, 209393

liver

Sus scrofa

-

-

209381, 209387, 209393

liver

Rattus norvegicus Sprague-Dawley

-

-

-

lung

Homo sapiens

-

-

209387, 209390

lung

Rattus norvegicus

-

-

209381, 209387, 209388, 209390

lung

Rattus norvegicus Sprague-Dawley

-

-

-

ovary

Oryctolagus cuniculus

-

-

209381

ovary

Homo sapiens

-

normal and cancer tissues

209389

root

Brassica rapa, Daucus carota, Raphanus sativus

-

-

209379

seed

Pisum sativum

-

-

209379

skeletal muscle

Rattus norvegicus

P97608

-

209388

skin

Homo sapiens, Mus musculus

-

-

209384

spleen

Oryctolagus cuniculus

-

-

209381

spleen

Rattus norvegicus

-

-

209381, 209387, 209388

spleen

Rattus norvegicus Sprague-Dawley

-

-

-

stomach

Homo sapiens

-

-

209390

testis

Rattus norvegicus

P97608

-

209388

tuber

Solanum tuberosum

-

-

209379

cytoplasm

Homo sapiens

-

-

5737

209390

cytoplasm

Rattus norvegicus

-

-

5737

209390, 209393

cytoplasm

Arabidopsis thaliana

Q9FIZ7

-

5737

689627

cytosol

Bos taurus

-, Q75WB5

-

5829

667886

115000

-

Pseudomonas putida

-

native molecular weight, component A, gel filtration

209373

137800

-

Rattus norvegicus

P97608

calculated from amino acid sequence

209388

150000

-

Bos taurus

-, Q75WB5

SDS-PAGE

667886

230000

-

Rattus norvegicus

-

zonal sedimentation in sucrose density gradient

209379, 209383

260000

-

Paecilomyces variotii

-

gel filtration

209394

280000

-

Saccharomyces cerevisiae

P28273, -

gel filtration

712045

325000

-

Rattus norvegicus

-

gel filtration

209372, 209375

325000

-

Rattus norvegicus

-

-

209379

325000

-

Rattus norvegicus

-

gel filtration

209381

325000

-

Rattus norvegicus

P97608

gel filtration

209388

420000

-

Rattus norvegicus

-

-

209375

460000

-

Rattus norvegicus

-

gel filtration

209379, 209383

650000

-

Pseudomonas putida

-

component B, gel filtration, native conditions

209373

750000

-

Pseudomonas putida

-

component A, gel filtration, native conditions

209373

dimer

Rattus norvegicus

-

-

209374

dimer

Rattus norvegicus

-

2 * 142000, SDS-PAGE

209372, 209375, 209383, 209388

dimer

Paecilomyces variotii

-

2 * 140000, SDS-PAGE

209394

dimer

Rattus norvegicus

-

2 * 140000, SDS-PAGE

209390, 209394

dimer

Saccharomyces cerevisiae

P28273, -

2 * 140000, SDS-PAGE

712045

dimer

Paecilomyces variotii F-1

-

2 * 140000, SDS-PAGE

-

polymer

Pseudomonas putida

-

2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE

209373

polymer

Pseudomonas putida

-

component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE

209388

tetramer

Rattus norvegicus

-

4 * 115000, SDS-PAGE

209375, 209383

6.5

8.5

Paecilomyces variotii

-

most stable at pH 7.5, loses activity rapidly below pH 5.5 and above pH 9

209394

50

-

Paecilomyces variotii

-

64% initial activity retains at 60°C, loses all initial activity at 70°C

209394

unstable in absence of 5-oxo-L-proline

Rattus norvegicus

-

209376, 209380, 209393

unstable in absence of 5-oxo-L-proline

Sus scrofa

-

209393

-70°C, no loss of activity detected

Homo sapiens

-

209389

4°C

Pseudomonas putida

-

209371

0°C, stable for up to 2 months

Pseudomonas sp.

-

209385

-80°C, no activity lost for up to 1 week

Rattus norvegicus

-

209390, 209393

4°C, 30 mM Tris-HCl buffer pH 7.8, 10 mM potassium chloride, 1 mM MgCl2, 5 mM 5-oxoproline, activity decreases to about 60% of its initial value after 2 days, 42% after 4 days and 40% after 5-6 days, addition of dithiothreitol leads to 104% of initial activity

Rattus norvegicus

-

209381

4°C, loses progressively activity, 60% initial activity lost in 6 days, activity completely restored upon addition of 5 mM dithiothreitol

Rattus norvegicus

-

209387

4°C, up to 30 h without decrease in activity

Rattus norvegicus

-

209393

Sepharyl S-300 gel filtration, Blue Sepharose and Phenyl Sepharose column chromatography

Bos taurus

-, Q75WB5

667886

-

Paecilomyces variotii

-

209394

-

Pseudomonas putida

-

209371

separate purification of components A and B

Pseudomonas putida

-

209373

-

Pseudomonas sp.

-

209384, 209385

-

Rattus norvegicus

-

209372, 209373, 209375, 209380, 209381, 209384, 209385, 209386, 209387, 209388, 209390

recombinant protein

Saccharomyces cerevisiae

P28273, -

712045

-

Sus scrofa

-

209392, 209393

-

Triticum aestivum

-

209379

cDNA in pT7-7, subcloned into mammalian expression vector pRc/CMV, transfected into MCF7

Homo sapiens

-

209391

amino acid sequence determined by cDNA cloning, gene expressed in Escherichia coli

Rattus norvegicus

P97608

209388

expression in Saccharamyces cerevisiae, N-terminally His-tagged

Saccharomyces cerevisiae

P28273, -

712045

additional information

Arabidopsis thaliana

Q9FIZ7

oxp1-1 and oxp1-2, oxoprolinase knockout mutants

689627

additional information

Saccharomyces cerevisiae

P28273, -

subunit consists of two distinct domains HyuA and HyuB. Cells of a met15 deletion strain expressing either the HyuA or the HyuB domains alone cannot utilize 5-oxoproline sulfur analogue OTC as sole source of sulfur. Cells coexpressing both HyuA and HyuB domains are able to grow on OTC as a sole source of sulfur, similar to cells expressing full-length yeast 5-oxoprolinase gene

712045

medicine

Homo sapiens

-

animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity

209386

medicine

Homo sapiens

-

reduces normal tissue toxicity of anticancer agents

209389

medicine

Homo sapiens

-

animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity; potenial implications for carcinogenesis and cancer chemotherapy

209390

medicine

Homo sapiens

-

clinical applications, substrate 5-oxo-L-proline sensitizes tumors to the alkylating agent melphalan

209391

medicine

Homo sapiens

-

5-oxoprolinuria is primarily associated with inborn errors of the gamma-glutamyl cycle

688174

medicine

Homo sapiens

-

occurrence of a futile cycle in patients with cystinosis. The enzyme gamma-glutamyl cysteine synthetase, in the absence of cysteine, forms 5-oxoproline instead of the normal substrate, gamma-glutamyl cysteine, and the 5-oxoproline is converted into glutamate by the ATP-dependant enzyme, 5-oxoprolinase. In cysteine-limiting conditions, glutamate is cycled back into glutamate via 5-oxoproline at the cost of two ATP molecules without production of glutathione and is the cause of the decreased levels of glutathione synthesis, as well as the ATP depletion observed in these cells

704751

medicine

Rattus norvegicus

-

animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity

209386, 209390

medicine

Rattus norvegicus

-

-

209393

medicine

Rattus norvegicus Sprague-Dawley

-

animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity

-

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM