Information on EC 3.5.2.9 - 5-oxoprolinase (ATP-hydrolysing)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.5.2.9
-
RECOMMENDED NAME
GeneOntology No.
5-oxoprolinase (ATP-hydrolysing)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
hydrolysis
Q9FIZ7
-
PATHWAY
KEGG Link
MetaCyc Link
gamma-glutamyl cycle
-
Glutathione metabolism
-
SYSTEMATIC NAME
IUBMB Comments
5-oxo-L-proline amidohydrolase (ATP-hydrolysing)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5-OPase
-
-
-
-
5-oxo-L-prolinase
-
-
-
-
5-oxo-L-prolinase
-
-
5-oxoprolinase
-
-
-
-
5-oxoprolinase
Q9FIZ7
-
5OPase
Q9FIZ7
-
L-pyroglutamate hydrolase
-
-
-
-
oxoprolinase
-
-
-
-
pyroglutamase
-
-
-
-
pyroglutamase (ATP-hydrolysing)
-
-
-
-
pyroglutamate hydrolase
-
-
-
-
pyroglutamic hydrolase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9075-46-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain F-137
-
-
Manually annotated by BRENDA team
Alcaligenes sp. F-137
strain F-137
-
-
Manually annotated by BRENDA team
wild-type plants and oxp1-1 and oxp2-2 mutants
UniProt
Manually annotated by BRENDA team
cow
-
-
Manually annotated by BRENDA team
cauliflower
-
-
Manually annotated by BRENDA team
turnip
-
-
Manually annotated by BRENDA team
squash
-
-
Manually annotated by BRENDA team
carrot
-
-
Manually annotated by BRENDA team
breast cancer cell line MCF7; human
-
-
Manually annotated by BRENDA team
mouse, NCS strain
-
-
Manually annotated by BRENDA team
Mus musculus NCS
mouse, NCS strain
-
-
Manually annotated by BRENDA team
strain F-1
-
-
Manually annotated by BRENDA team
Paecilomyces variotii F-1
strain F-1
-
-
Manually annotated by BRENDA team
strain ALA
-
-
Manually annotated by BRENDA team
Pseudomonas putida ALA
strain ALA
-
-
Manually annotated by BRENDA team
rat; strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
rat; strain Wistar albino
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats, female
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar albino
strain Wistar albino
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
P28273, -
comparison of the growth of 5-oxoprolinase deletion strains with wild-type strains in a variety of growth conditions does not yield any discernible phenotypes. Cells of glutamate auxotroph aco1D strain expressing OXP1 are able to grow on 5-oxoproline, although the growth is very slow
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-imidazolidone-4-carboxylate + ATP + H2O
2-imidazolidone + ADP + phosphate
show the reaction diagram
-
-
-
-
?
2-imidazolidone-4-carboxylate + ATP + H2O
2-imidazolidone + ADP + phosphate
show the reaction diagram
-
promotes enzymatic hydrolysis of ATP but is not itself hydrolyzed
-
-
?
2-imidazoline-4-carboxylate + ATP + H2O
2-imidazoline-4-carboxylate + ADP + phosphate
show the reaction diagram
-
does not undergo ring cleavage, but stimulates formation of inorganic phosphate from ATP
-
-
?
2-imidazolinone-4-carboxylate + H2O
?
show the reaction diagram
-
-
-
-
?
2-oxo-5,5-dimethylthiazolidine-4-carboxylate + H2O
?
show the reaction diagram
-
-
-
-
?
2-piperidone-6-carboxylate + ATP + H2O
2-aminoadipate + phosphate + ADP
show the reaction diagram
-
-
-
-
?
2-piperidone-6-carboxylate + ATP + H2O
2-aminoadipate + phosphate + ADP
show the reaction diagram
-
-
-
?
2-thiazolinone-4-carboxylate + H2O
?
show the reaction diagram
-
-
-
-
?
3-methyl-5-oxoproline + H2O
2-amino-3-methylpentanedioic acid
show the reaction diagram
-
-
-
-
?
3-oxy-5-oxoproline + H2O
3-oxyglutamate + phosphate
show the reaction diagram
-
-
-
-
?
3-oxy-5-oxoproline + H2O
3-oxyglutamate + phosphate
show the reaction diagram
-
-
-
?
4-methyl-5-oxoproline + H2O
2-amino-4-methylpentanedioic acid
show the reaction diagram
-
-
-
-
?
4-oxy-5-oxoproline + H2O
4-oxyglutamate + phosphate
show the reaction diagram
-
-
-
-
?
4-oxy-5-oxoproline + H2O
4-oxyglutamate + phosphate
show the reaction diagram
-
-
-
?
5'-p-fluorosulfonylbenzoyladenosine + H2O
?
show the reaction diagram
-
-
-
-
?
5'-p-fluorosulfonylbenzoylinosine + H2O
?
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + ATP + 2 H2O
L-glutamate + phosphate + ADP
show the reaction diagram
-
assay at pH 7.2, 5 mM substrate, 4C, 20 min
-
-
?
5-oxo-L-proline + ATP + H2O
L-glutamate + ADP + phosphate
show the reaction diagram
Q9FIZ7
assay at 30C, pH 9.5, reaction stopped by 20 microl acetic acid and heating for 5 min at 100C
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
P97608
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
ir
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Q75WB5
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
component F8 + component beta5
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations
-
-
ir
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
P28273, -
ATP is required
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Paecilomyces variotii F-1, Alcaligenes sp. F-137
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Mus musculus NCS
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Rattus norvegicus Wistar albino
-
-
-
-
-
alpha-hydroxyglutarate lactone + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
CTP + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida ALA
-
less than 5% of the activity observed with ATP
-
-
?
dATP + H2O
?
show the reaction diagram
-
-
-
-
?
dATP + H2O
?
show the reaction diagram
-
-
-
-
?
dATP + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
dATP + H2O
?
show the reaction diagram
Pseudomonas putida ALA
-
-
-
-
?
DL-2-oxo-3,3-dimethylthiazolidine-4-carboxylate + H2O
?
show the reaction diagram
-
-
-
-
?
DL-cis-2-oxo-5-methyloxazolidine-4-carboxylate + H2O
DL-threonine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
GTP + H2O
?
show the reaction diagram
-
-
-
-
?
GTP + H2O
?
show the reaction diagram
-
less than 5% of the activity observed with ATP
-
-
?
GTP + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
GTP + H2O
?
show the reaction diagram
Pseudomonas putida ALA
-
less than 5% of the activity observed with ATP
-
-
?
ITP + H2O
?
show the reaction diagram
-
-
-
-
?
ITP + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
L-2-iminothiazolidine-4-carboxylate + H2O
L-cystine
show the reaction diagram
-
-
-
-
?
L-2-oxooxazolidine-4-carboxylate + H2O + ATP
L-serine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
L-cysteine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
L-cysteine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
L-cysteine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
L-cysteine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
L-cysteine + ADP + phosphate
show the reaction diagram
Alcaligenes sp., Paecilomyces variotii F-1, Alcaligenes sp. F-137
-
-
-
-
?
L-dihydroorotate + H2O
?
show the reaction diagram
-
do not undergo ring cleavage, but stimulates formation of inorganic phosphate from ATP
-
-
?
L-trans-2-oxo-5-methyloxazolidine-4-carboxylate + H2O
L-threonine + ADP + phosphate
show the reaction diagram
-
-
-
-
?
MgATP2- + H2O
?
show the reaction diagram
-
-
-
-
-
MgATP2- + H2O
?
show the reaction diagram
-
-
-
-
?
MgATP2- + H2O
?
show the reaction diagram
-
-
-
-
-
MgATP2- + H2O
?
show the reaction diagram
-
-
-
-
-
UTP + H2O
?
show the reaction diagram
-
-
-
-
?
UTP + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida ALA
-
less than 5% of the activity observed with ATP
-
-
?
XTP + H2O
?
show the reaction diagram
-
less than 5% of the activity observed with ATP
-
-
?
MgATP2- + H2O
?
show the reaction diagram
-
component beta5
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
L-thioproline is no substrate
-
-
-
additional information
?
-
-
5-oxo-D-proline, 2-pyrrolidone-4-carboxylate, N-methyl-5-oxo-L-proline and N-acetyl-L-alanine are no substrates
-
-
-
additional information
?
-
-
L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates
-
-
-
additional information
?
-
-
CTP, TTP and UTP are no substrates
-
-
-
additional information
?
-
-
CTP and UTP are poor substrates
-
-
-
additional information
?
-
-
no activity observed with 5-oxo-D-proline
-
-
-
additional information
?
-
Rattus norvegicus Sprague-Dawley
-
L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates
-
-
-
additional information
?
-
Paecilomyces variotii F-1
-
CTP, TTP and UTP are no substrates
-
-
-
additional information
?
-
Pseudomonas putida ALA
-
no activity observed with 5-oxo-D-proline
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
P97608
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
-
conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations
-
-
ir
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
-
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Paecilomyces variotii F-1, Alcaligenes sp. F-137
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Mus musculus NCS
-
-
-
-
?
5-oxo-L-proline + H2O + ATP
L-glutamate + ADP + phosphate
show the reaction diagram
Rattus norvegicus Wistar albino
-
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
promotes activity significantly
Co2+
-
promotes activity significantly
FeSO4
-
1 mM , relative activity 113%
K+
-
maximal activity of component A as an ATPase requires 80-300 mM
K+
-
requires monovalent cation, K+ or NH4+
K+
-
requires monovalent cation, K+ or NH4+
K+
-
requires monovalent cation, K+ or NH4+
Mn2+
-
1 mM MnCl2, relative activity 144%
additional information
P28273, -
enzyme requires ATP, a monovalent cation such as K+ or NH4+ and a divalent cation such as Mg2+ or Mn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3-methyl-5-oxo-L-proline
-
-
5-oxo-L-proline
-
inhibition of IPTase activity
DL-cis-2-oxo-5-methyloxazolidine-4-carboxylate
-
inhibition of IPTase activity
DTNB
-
could be reactivated with dithiothreitol
imidazolone carboxylate
-
-
iodoacetamide
-
-
L-2-imidazolidone-4-carboxylate
-
-
L-2-imidazolidone-4-carboxylate
-
-
L-2-imidazolidone-4-carboxylate
-
-
L-2-iminothiazolidine-4-carboxylate
-
inhibition of IPTase activity
L-2-oxooxazolidine-4-carboxylate
-
inhibition of IPTase activity
L-2-Oxothiazolidine-4-carboxylate
-
competitive inhibitor
L-2-Oxothiazolidine-4-carboxylate
-
-
L-2-Oxothiazolidine-4-carboxylate
-
-
L-2-Oxothiazolidine-4-carboxylate
-
inhibition of IPTase activity
L-2-Oxothiazolidine-4-carboxylate
-
competitive inhibitor
L-glutamine
-
inhibition of enzyme activity in tumor tissue
L-trans-2-oxo-5-methyloxazolidine-4-carboxylate
-
inhibition of glutamate formation; inhibition of IPTase activity
-
N-bromosuccinimide
-
-
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-imidazolidone-4-carboxylate
-
5-oxoproline analog, stimulates rapid ATP cleavage, but is not hydrolyzed itself
2-piperidone-6-carboxylate
-
stimulates ATP cleavage
L-glutamine
-
enhanced enzyme activity in jejunal mucosa of tumor-bearing rats
NH2OH-HCl
-
1 mM, relative activity 109%
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.005
-
5-oxo-L-proline
-
-
0.008
-
5-oxo-L-proline
-
-
0.008
-
5-oxo-L-proline
-
-
0.009
-
5-oxo-L-proline
-
treated with N-ethylmaleimide
0.014
-
5-oxo-L-proline
-
-
0.043
-
5-oxo-L-proline
-
-
0.0511
-
5-oxo-L-proline
-
-
0.0316
-
5-oxoproline
-
-
0.05
-
5-oxoproline
-
-
0.07
-
5-oxoproline
-
-
0.14
-
5-oxoproline
-
-
0.16
-
5-oxoproline
-
initial Km in presence of GTP
0.18
-
5-oxoproline
-
initial Km in presence of ITP
0.2
-
5-oxoproline
-
initial Km in presence of UTP
0.41
-
5-oxoproline
-
in presence of ATP
0.5
-
5-oxoproline
-
final Km in presence of UTP
0.5
-
5-oxoproline
-
-
0.57
-
5-oxoproline
-
final Km in presence of GTP
0.58
-
5-oxoproline
-
final Km in presence of ITP
0.03
-
ATP
-
without 5-oxoproline
0.091
-
ATP
-
5-oxo-L-proline as substrate
0.13
-
ATP
-
measured in absence of 5-oxoproline
0.21
-
ATP
-
L-2-oxothiazolidine-4-carboxylate as substrate
0.42
-
ATP
-
5-oxoproline added
0.13
-
ITP
-
measured in absence of 5-oxoproline
0.17
-
ITP
-
without 5-oxoproline
0.41
-
ITP
-
5-oxoproline added
0.17
-
L-2-Oxothiazolidine-4-carboxylate
-
-
0.002
-
L-2-oxothiazolidine-4-carboxylic acid
-
-
0.002
-
L-2-oxothiazolidine-4-carboxylic acid
-
-
0.1
-
MgATP2-
-
-
0.1727
-
MgATP2-
-
-
4.2
-
MgATP2-
-
-
0.5
-
MnATP2-
-
-
0.17
-
UTP
-
without 5-oxoproline
1.11
-
UTP
-
5-oxoproline added
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.117
-
5-oxo-L-proline
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00195
-
-, Q75WB5
cytosolic fraction of kidney
0.625
-
-, Q75WB5
after purification by Sepharyl S-300, Blue Sepharose and Phenyl Sepharose column chromatography
1
-
-
measured in tumor tissue after treatment with dietary glutamine
1.25
-
-
measured in jejunal mucosa in DMBA-teated rats with tumor
2.5
-
-
measured in tumor tissue
2.91
-
-
measured in jejunal mucosa in DMBA-treated rats without tumor
3
-
-
measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats without tumor
3.6
-
-
-
3.75
-
-
measured in jejunal mucosa in normal rats
4.17
-
-
measured in jejunal mucosa after treatment with dietary glutamine in normal rats
4.33
-
-
measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats with tumor
75
-
-
component F8
75
-
-
purified component B
243
-
-
component beta5
243
-
-
purified component A
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
second pH optimum at pH 9.7
7.2
-
-
assay at
7.8
8
-
Tris-HCl buffer
8.2
-
-
component beta5, cleavage of ATP
8.2
-
-
component A-catalyzed 5-oxoproline-dependent cleavage of ATP
9.5
-
-
component F8, ATP-dependent conversion of 5-oxoproline to glutamate
9.5
-
-
component B
9.5
-
Q9FIZ7
assay at
9.7
-
-
second pH optimum at pH 7.0
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
11.3
-
-
5.5
11.2
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
-
assay at
30
-
Q9FIZ7
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
peripheral mononuclear cells
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
normal and cancer tissues
Manually annotated by BRENDA team
-
normal and cancer tissues
Manually annotated by BRENDA team
Mus musculus NCS
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
;
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar albino
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
normal and cancer tissues
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
115000
-
-
native molecular weight, component A, gel filtration
137800
-
P97608
calculated from amino acid sequence
150000
-
-, Q75WB5
SDS-PAGE
230000
-
-
zonal sedimentation in sucrose density gradient
260000
-
-
gel filtration
280000
-
P28273, -
gel filtration
325000
-
-
gel filtration
325000
-
-
-
325000
-
-
gel filtration
325000
-
P97608
gel filtration
420000
-
-
-
460000
-
-
gel filtration
650000
-
-
component B, gel filtration, native conditions
750000
-
-
component A, gel filtration, native conditions
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 142000, SDS-PAGE
dimer
-
2 * 140000, SDS-PAGE
dimer
-
2 * 140000, SDS-PAGE
dimer
P28273, -
2 * 140000, SDS-PAGE
dimer
Paecilomyces variotii F-1
-
2 * 140000, SDS-PAGE
-
polymer
-
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE
polymer
-
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE
tetramer
-
4 * 115000, SDS-PAGE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8.5
-
most stable at pH 7.5, loses activity rapidly below pH 5.5 and above pH 9
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
64% initial activity retains at 60C, loses all initial activity at 70C
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable in absence of 5-oxo-L-proline
-
unstable in absence of 5-oxo-L-proline
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70C, no loss of activity detected
-
0C, stable for up to 2 months
-
-80C, no activity lost for up to 1 week
-
4C, 30 mM Tris-HCl buffer pH 7.8, 10 mM potassium chloride, 1 mM MgCl2, 5 mM 5-oxoproline, activity decreases to about 60% of its initial value after 2 days, 42% after 4 days and 40% after 5-6 days, addition of dithiothreitol leads to 104% of initial activity
-
4C, loses progressively activity, 60% initial activity lost in 6 days, activity completely restored upon addition of 5 mM dithiothreitol
-
4C, up to 30 h without decrease in activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Sepharyl S-300 gel filtration, Blue Sepharose and Phenyl Sepharose column chromatography
-, Q75WB5
separate purification of components A and B
-
recombinant protein
P28273, -
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cDNA in pT7-7, subcloned into mammalian expression vector pRc/CMV, transfected into MCF7
-
amino acid sequence determined by cDNA cloning, gene expressed in Escherichia coli
P97608
expression in Saccharamyces cerevisiae, N-terminally His-tagged
P28273, -
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
Q9FIZ7
oxp1-1 and oxp1-2, oxoprolinase knockout mutants
additional information
P28273, -
subunit consists of two distinct domains HyuA and HyuB. Cells of a met15 deletion strain expressing either the HyuA or the HyuB domains alone cannot utilize 5-oxoproline sulfur analogue OTC as sole source of sulfur. Cells coexpressing both HyuA and HyuB domains are able to grow on OTC as a sole source of sulfur, similar to cells expressing full-length yeast 5-oxoprolinase gene
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity
medicine
-
reduces normal tissue toxicity of anticancer agents
medicine
-
animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity; potenial implications for carcinogenesis and cancer chemotherapy
medicine
-
clinical applications, substrate 5-oxo-L-proline sensitizes tumors to the alkylating agent melphalan
medicine
-
5-oxoprolinuria is primarily associated with inborn errors of the gamma-glutamyl cycle
medicine
-
occurrence of a futile cycle in patients with cystinosis. The enzyme gamma-glutamyl cysteine synthetase, in the absence of cysteine, forms 5-oxoproline instead of the normal substrate, gamma-glutamyl cysteine, and the 5-oxoproline is converted into glutamate by the ATP-dependant enzyme, 5-oxoprolinase. In cysteine-limiting conditions, glutamate is cycled back into glutamate via 5-oxoproline at the cost of two ATP molecules without production of glutathione and is the cause of the decreased levels of glutathione synthesis, as well as the ATP depletion observed in these cells
medicine
-
animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity
medicine
-
-
medicine
Rattus norvegicus Sprague-Dawley
-
animal model for the human condition 5-oxoprolinuria, an inborn deficiency of renal 5-oxoprolinase activity
-