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Literature summary for 3.5.2.9 extracted from
- 5-Oxo-L-prolinase from Pseudomonas putida (1985), Methods Enzymol., 113, 451-458.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
L-2-oxothiazolidine-4-carboxylic acid | - |
Rattus norvegicus |  |
| 0.002 | - |
L-2-oxothiazolidine-4-carboxylic acid | - |
Pseudomonas putida | |
| 0.008 | - |
5-oxo-L-proline | - |
Rattus norvegicus | |
| 0.008 | - |
5-oxo-L-proline | - |
Pseudomonas putida | |
| 0.043 | - |
5-oxo-L-proline | - |
Pseudomonas putida | |
| 0.15 | - |
MgATP2- | - |
Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida ALA | - |
- |
- |
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 75 | - |
component F8 | Pseudomonas putida |
| 243 | - |
component beta5 | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
? | |
| 5-oxo-L-proline + H2O + ATP | component F8 + component beta5 | Pseudomonas putida | L-glutamate + ADP + phosphate | - |
? | |
| CTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida | ? | - |
? | |
| CTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida ALA | ? | - |
? | |
| dATP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| dATP + H2O | - |
Pseudomonas putida | ? | - |
? | |
| dATP + H2O | - |
Pseudomonas putida ALA | ? | - |
? | |
| GTP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| GTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida | ? | - |
? | |
| GTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida ALA | ? | - |
? | |
| L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Rattus norvegicus | L-cysteine + ADP + phosphate | - |
? | |
| L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Pseudomonas putida | L-cysteine + ADP + phosphate | - |
? | |
| L-2-oxothiazolidine-4-carboxylic acid + H2O | - |
Rattus norvegicus | L-cysteine + ADP + phosphate | - |
? | |
| L-2-oxothiazolidine-4-carboxylic acid + H2O | - |
Pseudomonas putida | L-cysteine + ADP + phosphate | - |
? | |
| MgATP2- + H2O | - |
Rattus norvegicus | ? | - |
? | |
| MgATP2- + H2O | component beta5 | Pseudomonas putida | ? | - |
? | |
| additional information | no activity observed with 5-oxo-D-proline | Pseudomonas putida | ? | - |
? | |
| additional information | no activity observed with 5-oxo-D-proline | Pseudomonas putida ALA | ? | - |
? | |
| UTP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| UTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida | ? | - |
? | |
| UTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida ALA | ? | - |
? | |
| XTP + H2O | less than 5% of the activity observed with ATP | Pseudomonas putida | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
component beta5, cleavage of ATP | Pseudomonas putida |
| 9.5 | - |
component F8, ATP-dependent conversion of 5-oxoproline to glutamate | Pseudomonas putida |
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