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Literature summary for 3.5.2.9 extracted from
- The 5-oxoprolinase is required for conidiation, sexual reproduction, virulence and deoxynivalenol production of Fusarium graminearum (2018), Curr. Genet., 64, 285-301 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FGSG_04902, phylogenetic analysis | Fusarium graminearum |
| gene FGSG_10203, sequence comparisons and phylogenetic analysis | Fusarium graminearum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of isozyme FgOXP1 deletion mutant, DELTAFgoxp1, and of double deletion mutant DELTAFgoxp1/Fgoxp2. Construction of GFP-tagged FgOXP1/2 complementary strains of Fusarium graminearum | Fusarium graminearum |
| additional information | construction of isozyme FgOXP2 deletion mutant, DELTAFgoxp2, and of double deletion mutant DELTAFgoxp1/Fgoxp2. Construction of GFP-tagged FgOXP1/2 complementary strains of Fusarium graminearum | Fusarium graminearum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Fusarium graminearum | 5737 | - |
| additional information | isozymes FgOxp1 and FgOxp2 have different intracellular distributions. No matter during which developmental stage, GFP-tagged FgOxp2 localizes evenly in the cytoplasm but not to vacuoles, while GFP-tagged FgOxp1 localizes in the cytoplasm much more unevenly. During matured conidial stage, FgOxp1-GFP often accumulates into spherical structures in clustered pattern, and during the vegetative hypha stage, some GFP-tagged FgOxp1 even accumulates close to vacuoles as bright dots | Fusarium graminearum | - |
- |
| vacuole | - |
Fusarium graminearum | 5773 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Fusarium graminearum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-oxo-L-proline + 2 H2O | Fusarium graminearum | - |
ADP + phosphate + L-glutamate | - |
? | |
| ATP + 5-oxo-L-proline + 2 H2O | Fusarium graminearum PH-1 | - |
ADP + phosphate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium graminearum | I1RLT3 | i.e. Gibberella zeae | - |
| Fusarium graminearum | I1S0H5 | i.e. Gibberella zeae | - |
| Fusarium graminearum PH-1 | I1RLT3 | i.e. Gibberella zeae | - |
| Fusarium graminearum PH-1 | I1S0H5 | i.e. Gibberella zeae | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ascus | - |
Fusarium graminearum | - |
| additional information | FgOxp1 shows a low level of expression in perithecia, asci rosettes, and ascus | Fusarium graminearum | - |
| mycelium | - |
Fusarium graminearum | - |
| perithecium | - |
Fusarium graminearum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-oxo-L-proline + 2 H2O | - |
Fusarium graminearum | ADP + phosphate + L-glutamate | - |
? | |
| ATP + 5-oxo-L-proline + 2 H2O | - |
Fusarium graminearum PH-1 | ADP + phosphate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | domain architecture, overview | Fusarium graminearum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5-oxoprolinase | - |
Fusarium graminearum |
| FgOXP1 | - |
Fusarium graminearum |
| FgOXP2 | - |
Fusarium graminearum |
| FGSG_04902 | - |
Fusarium graminearum |
| FGSG_10203 | - |
Fusarium graminearum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Fusarium graminearum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | detailed phylogenetic analysis of 5-oxoprolinases, overview | Fusarium graminearum |
| malfunction | deletion of FgOXP1 or FgOXP2 in Fusarium graminearum leads to significant defects in its virulence on wheat, likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains. DON is one of the best characterized virulence factors of Fusarium graminearum. The FgOXP2 deletion mutant strains are also defective in conidiation and sexual reproduction while the FgOXP1 deletion mutant strains are normal for those phenotypes. Double deletion of FgOXP1 and FgOXP2 leads to more severe defects in conidiation, DON production and virulence on plants, suggesting that both FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. Although transformation of the enzyme from Magnaporthe oryzae wild-type strain 70-15, MoOXP1, into DELTAFgoxp1 is able to complement DELTAFgoxp1, transformation of MoOXP1 into DELTAFgoxp2 fails to restore its defects in sexual development, DON production, and pathogenicity. Defects noticed in the gene deletion mutant strains of 5-oxoprolinase in Fusarium graminearum are caused by the affected gamma-glutamyl cycle, phenotypes, overview | Fusarium graminearum |
| malfunction | deletion of FgOXP1 or FgOXP2 in Fusarium graminearum leads to significant defects in its virulence on wheat, likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains. DON is one of the best characterized virulence factors of Fusarium graminearum. The FgOXP2 deletion mutant strains are also defective in conidiation and sexual reproduction while the FgOXP1 deletion mutant strains are normal for those phenotypes. Double deletion of FgOXP1 and FgOXP2 leads to more severe defects in conidiation, DON production and virulence on plants, suggesting that both FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. Although transformation of the enzyme from Magnaporthe oryzae wild-type strain 70-15, MoOXP1, into DELTAFgoxp1, transformation of MoOXP1 into DELTAFgoxp2 fails to restore its defects in sexual development, DON production, and pathogenicity. Defects noticed in the gene deletion mutant strains of 5-oxoprolinase in Fusarium graminearum are caused by the affected gamma-glutamyl cycle, phenotypes, overview | Fusarium graminearum |
| physiological function | 5-oxoprolinase is required for conidiation, sexual reproduction, virulence, and deoxynivalenol production of Fusarium graminearum. Both isozymes FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. FgOXP1 and FgOXP2 function differentially in deoxynivalenol DON production and in sexual reproduction of Fusarium graminearum, overview | Fusarium graminearum |
| physiological function | 5-oxoprolinase is required for conidiation, sexual reproduction, virulence, and deoxynivalenol production of Fusarium graminearum. Both isozymes FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. FgOXP1 and FgOXP2 function differentially in deoxynivalenol DON production and in sexual reproduction of Fusarium graminearum, overview. A clearly much stronger role for Oxp2 in perithecia and ascus formation | Fusarium graminearum |
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