Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | could be reactivated with dithiothreitol | Rattus norvegicus | |
N-ethylmaleimide | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
5-oxo-L-proline | - |
Rattus norvegicus | |
0.009 | - |
5-oxo-L-proline | treated with N-ethylmaleimide | Rattus norvegicus | |
0.13 | - |
ITP | measured in absence of 5-oxoproline | Rattus norvegicus | |
0.17 | - |
ATP | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | promotes activity significantly | Rattus norvegicus | |
Co2+ | promotes activity significantly | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
4 * 115000, SDS-PAGE | Rattus norvegicus |
142000 | - |
2 * 142000, SDS-PAGE | Rattus norvegicus |
325000 | - |
gel filtration | Rattus norvegicus |
420000 | - |
- |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-oxo-L-proline + H2O + ATP | Rattus norvegicus | - |
L-glutamate + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |
87 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-imidazolidone-4-carboxylate + ATP + H2O | promotes enzymatic hydrolysis of ATP but is not itself hydrolyzed | Rattus norvegicus | 2-imidazolidone + ADP + phosphate | - |
? | |
5'-p-fluorosulfonylbenzoyladenosine + H2O | - |
Rattus norvegicus | ? | - |
? | |
5'-p-fluorosulfonylbenzoylinosine + H2O | - |
Rattus norvegicus | ? | - |
? | |
5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
? | |
ATP + H2O | - |
Rattus norvegicus | ? | - |
? | |
GTP + H2O | - |
Rattus norvegicus | ? | - |
? | |
ITP + H2O | - |
Rattus norvegicus | ? | - |
? | |
UTP + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 142000, SDS-PAGE | Rattus norvegicus |
tetramer | 4 * 115000, SDS-PAGE | Rattus norvegicus |