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Literature summary for 3.5.2.9 extracted from

  • Mazelis, M.; Creveling, R.G.
    5-Oxoprolinase (L-pyroglutamate hydrolase) in higher plants (1978), Plant Physiol., 62, 798-801.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.9

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Rattus norvegicus
iodoacetamide
-
 Triticum aestivum
L-2-imidazolidone-4-carboxylate
-
 Rattus norvegicus
L-2-imidazolidone-4-carboxylate
-
 Triticum aestivum
N-ethylmaleimide
-
 Rattus norvegicus
N-ethylmaleimide
-
 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 5-oxo-L-proline
-
 Triticum aestivum
0.14
-
 5-oxoproline
-
 Pseudomonas sp.
0.17
-
 ATP
-
 Rattus norvegicus
0.17
-
 ATP
-
 Triticum aestivum
0.4
-
 ATP
-
 Triticum aestivum
0.5
-
 5-oxoproline
-
 Rattus norvegicus
0.5
-
 MnATP2-
-
 Triticum aestivum
1
-
 ATP
-
 Pseudomonas sp.
4.2
-
 MgATP2-
-
 Triticum aestivum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requires monovalent cation, K+ or NH4+ Rattus norvegicus
K+ requires monovalent cation, K+ or NH4+ Triticum aestivum
K+ requires monovalent cation, K+ or NH4+ Pseudomonas sp.
Mg2+
-
 Rattus norvegicus
Mg2+
-
 Triticum aestivum
Mn2+
-
 Rattus norvegicus
Mn2+
-
 Triticum aestivum
Rb+
-
 Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
 zonal sedimentation in sucrose density gradient Rattus norvegicus
325000
-
-
 Rattus norvegicus
460000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Triticum aestivum
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Spinacia oleracea
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Pisum sativum
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Solanum tuberosum
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Pseudomonas sp.
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Daucus carota
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Raphanus sativus
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Brassica oleracea
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Cucurbita pepo
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Brassica rapa
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 cauliflower
-
Brassica rapa
-
 turnip
-
Cucurbita pepo
-
 squash
-
Daucus carota
-
 carrot
-
Pisum sativum
-
 pea
-
Pseudomonas sp.
-
-
-
Raphanus sativus
-
 radish
-
Rattus norvegicus
-
 rat
-
Solanum tuberosum
-
 potato
-
Spinacia oleracea
-
 spinach
-
Triticum aestivum
-
 wheat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
bud
-
 Brassica oleracea
-
fruit
-
 Cucurbita pepo
-
germ
-
 Triticum aestivum
-
kidney
-
 Rattus norvegicus
-
leaf
-
 Spinacia oleracea
-
leaf
-
 Brassica rapa
-
root
-
 Daucus carota
-
root
-
 Raphanus sativus
-
root
-
 Brassica rapa
-
seed
-
 Pisum sativum
-
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.1
-
-
 Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Triticum aestivum L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Spinacia oleracea L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Pisum sativum L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Solanum tuberosum L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Pseudomonas sp. L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Daucus carota L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Raphanus sativus L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Brassica oleracea L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Cucurbita pepo L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Brassica rapa L-glutamate + ADP + phosphate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O ATP in form of MnATP2- Triticum aestivum ADP + phosphate + L-glutamate
-
 ?
ATP + H2O
-
 Rattus norvegicus ?
-
 ?
ATP + H2O
-
 Triticum aestivum ?
-
 ?
GTP + H2O
-
 Rattus norvegicus ?
-
 ?
GTP + H2O
-
 Triticum aestivum ?
-
 ?
ITP + H2O
-
 Rattus norvegicus ?
-
 ?
ITP + H2O
-
 Triticum aestivum ?
-
 ?
MgATP2- + H2O
-
 Triticum aestivum ?
-
 ?
additional information CTP and UTP are poor substrates Triticum aestivum ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Triticum aestivum