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Literature summary for 3.5.2.9 extracted from
- Kinetic parameters and tissue distribution of 5-oxo-L-prolinase determined by a fluorimetric assay (1999), J. Biochem. Biophys. Methods, 38, 71-82.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| unstable in absence of 5-oxo-L-proline | Rattus norvegicus |
| unstable in absence of 5-oxo-L-proline | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
5-oxo-L-proline | - |
Rattus norvegicus |  |
| 0.05 | - |
5-oxoproline | - |
Rattus norvegicus | |
| 0.0511 | - |
5-oxo-L-proline | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | Rattus norvegicus | - |
L-glutamate + ADP + phosphate | - |
? | |
| 5-oxo-L-proline + H2O + ATP | Sus scrofa | - |
L-glutamate + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| brain | - |
Sus scrofa | - |
| cardiac muscle | - |
Rattus norvegicus | - |
| cardiac muscle | - |
Sus scrofa | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Sus scrofa | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, no activity lost for up to 1 week | Rattus norvegicus |
| 4°C, up to 30 h without decrease in activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
? | |
| 5-oxo-L-proline + H2O + ATP | - |
Sus scrofa | L-glutamate + ADP + phosphate | - |
? | |
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Release 2023.1 (January 2023)
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