Information on EC 1.11.1.21 - catalase-peroxidase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.11.1.21
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RECOMMENDED NAME
GeneOntology No.
catalase-peroxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 H2O2 = O2 + 2 H2O
show the reaction diagram
donor + H2O2 = oxidized donor + 2 H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methane metabolism
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Phenylalanine metabolism
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Tryptophan metabolism
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Phenylpropanoid biosynthesis
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Biosynthesis of secondary metabolites
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superoxide radicals degradation
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methanol oxidation to formaldehyde IV
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reactive oxygen species degradation
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SYSTEMATIC NAME
IUBMB Comments
donor:hydrogen-peroxide oxidoreductase
Differs from EC 1.11.1.7, peroxidase in having a relatively high catalase (EC 1.11.1.6) activity with H2O2 as donor, releasing O2; both activities use the same heme active site. In Mycobacterium tuberculosis it is responsible for activation of the commonly used antitubercular drug, isoniazid.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
formerly Acinetobacter sp. strain JC1 DSM 3803
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Manually annotated by BRENDA team
formerly Acinetobacter sp. strain JC1 DSM 3803
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Manually annotated by BRENDA team
formerly Synechococcus sp. strain PCC 6301
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
gene katG, KatG is a bifunctional enzyme showing both catalase and peroxidase activities
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 H2O2
O2 + 2 H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
? + H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + tert-butyl hydroperoxide
oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + ?
show the reaction diagram
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-
-
-
?
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
?
show the reaction diagram
2,4-dichlorophenol + H2O2
?
show the reaction diagram
-
-
-
?
2,6-dimethoxyphenol + H2O2
oxidized 2,6-dimethoxyphenol + H2O
show the reaction diagram
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-
-
-
?
2-dianisidine + tert-butyl hydroperoxide
?
show the reaction diagram
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peroxidase activity
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?
3,3',5,5'-tetramethylbenzidine + H2O2
? + H2O
show the reaction diagram
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-
-
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?
3,3',5,5'-tetramethylbenzidine + H2O2
oxidized 3,3',5,5'-tetramethylbenzidine + H2O
show the reaction diagram
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-
-
-
?
3,3'-diaminobenzidine + H2O2
oxidized 3,3'-diaminobenzidine + H2O
show the reaction diagram
3,3'-dimethoxybenzidine + H2O2
oxidized 3,3'-dimethoxybenzidine + H2O
show the reaction diagram
3-chloro-peroxybenzoic acid
?
show the reaction diagram
-
-
-
-
?
3-chloroperoxybenzoic acid
?
show the reaction diagram
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-
-
-
?
3-methoxyphenol + H2O2
? + H2O
show the reaction diagram
4-aminoantipyrine + H2O2
oxidized 4-aminoantipyrine + H2O
show the reaction diagram
4-phenylenediamine + H2O2
oxidized 4-phenylenediamine + H2O
show the reaction diagram
aminoantipyrine + H2O2
oxidized aminoantipyrine + H2O
show the reaction diagram
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-
-
?
aniline + H2O2
? + H2O
show the reaction diagram
ascorbate + H2O2
monodehydroascorbate + H2O
show the reaction diagram
catechol + H2O2
? + H2O
show the reaction diagram
chlorpromazine + H2O2
? + H2O
show the reaction diagram
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?
diaminobenzidine + H2O2
oxidized diaminobenzidine + H2O
show the reaction diagram
ethanol + H2O2
acetaldehyde + ?
show the reaction diagram
guaiacol + H2O2
?
show the reaction diagram
guaiacol + H2O2
oxidized guaiacol + H2O
show the reaction diagram
guajacol + H2O2
? + H2O
show the reaction diagram
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peroxidase activity
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?
H2O2
O2 + H2O
show the reaction diagram
H2O2 + o-dianisidine
?
show the reaction diagram
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peroxidase activity
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?
isonazid + H2O2
?
show the reaction diagram
isoniazid + H2O2
oxidized isoniazid + H2O
show the reaction diagram
isoniazid + NADH
isonicotinic acyl-NADH
show the reaction diagram
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isoniazid activation
the product is a strong inhibitor of the NADH-dependent enoyl-[acyl carrier protein] reductase InhA and the beta-ketoacyl [acyl carrier protein] synthase
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?
methanol + H2O2
formaldehyde + H2O
show the reaction diagram
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at pH 4.5
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?
N,N,N',N'-tetramethyl-p-phenylenediamine + H2O2
? + H2O
show the reaction diagram
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?
NADH + H2O2
?
show the reaction diagram
NADPH + H2O2
?
show the reaction diagram
o-dianisidine + H2O2
?
show the reaction diagram
o-dianisidine + H2O2
? + H2O
show the reaction diagram
o-dianisidine + H2O2
oxidized o-dianisidine + H2O
show the reaction diagram
o-dianisidine + tert-butyl hydroperoxide
o-dianisidine quinonediimine + ?
show the reaction diagram
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?
o-dianisidine + tert-butyl hydroperoxide
oxidized o-dianisidine + ?
show the reaction diagram
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?
penicillin G + H2O2
penicillin G (R)-sulfoxide + H2O
show the reaction diagram
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?
peroxyacetic acid
?
show the reaction diagram
phenol + H2O2
? + H2O
show the reaction diagram
pyrogallol + H2O2
?
show the reaction diagram
pyrogallol + H2O2
? + H2O
show the reaction diagram
reduced 2,3',6'-trichloroindophenol + H2O2
oxidized 2,3',6'-trichloroindophenol + H2O
show the reaction diagram
reduced cytochrome c + H2O2
oxidized cytochrome c + H2O
show the reaction diagram
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?
tert-butyl hydroperoxide + H2O2
oxidized tert-butyl hydroperoxide + H2O
show the reaction diagram
tert-butyl peroxide + H2O2
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
H2O2
O2 + H2O
show the reaction diagram
isonazid + H2O2
?
show the reaction diagram
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activation of antituberculosis drug isonazid, catalytic mechanism
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CO
-
complexed with the enzyme, structure determination, binding kinetics
Fe
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dioxygen binding to ferrous KatG and Y249F is reversible and monophasic. Ferrous wild-type KatG is rapidly converted by hydrogen peroxide in a two-phasic reaction via compound II to compound III, the latter being also efficiently transformed to ferric KatG. Determination of bimolecular rate constant and dissociation constant
K+
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2 mM K+ have a stimulatory effect on catalase activity
Mg2+
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2 mM Mg2+ have a stimulatory effect on catalase activity
NO
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complexed with the enzyme, structure determination, binding kinetics
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
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acts as an inhibitor of peroxidase activity at lower pH (pH 3.8-4.5)
3-Amino-1,2,4-triazole
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Ba2+
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partial inhibition of catalase activity
Co2+
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partial inhibition of catalase activity
cyanide
histidine
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19% inhibition at 10 mM
hydroxylamine
Isoniazid
Isonicotinic acid hydrazide
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nicotinic acid hydrazide
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strong competitive inhibition
Sodium azide
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25 mM, 30% inhibition of peroxidase activity, 100% inhibition of catalyse activity
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-1,2,4-triazole
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156% relative catalase activity at 10 mM, 121% relative peroxidase activity at 10 mM
ammonium sulfate
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additions of ammonium sulfate even at weak concentrations (10 to 70 mM) stimulate catalatic activity measured in the presence of 1.5 M NaC1 by about 30%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055 - 6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
0.063 - 0.096
2-dianisidine
0.7
3,3',5,5'-tetramethylbenzidine
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pH 5.0, 23°C
0.13
ascorbate
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pH 5.0, 23°C
1.1
chlorpromazine
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pH 5.0, 23°C
0.06 - 225
H2O2
0.08 - 0.43
Isoniazid
1.2
N,N,N',N'-tetramethyl-p-phenylenediamine
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pH 5.0, 23°C
0.23
NADH
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peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C
0.17
NADPH
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peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C
0.04 - 0.49
o-Dianisidine
0.2 - 1.6
pyrogallol
5.7 - 97.9
tert-butyl hydroperoxide
additional information
additional information
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steady- and transient-state kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34 - 210000
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
0.86 - 4.85
2-dianisidine
5220
3,3',5,5'-tetramethylbenzidine
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pH 5.0, 23°C
520
ascorbate
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pH 5.0, 23°C
7940
chlorpromazine
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pH 5.0, 23°C
0.0061 - 18000
H2O2
0.057 - 0.081
Isoniazid
7012
N,N,N',N'-tetramethyl-p-phenylenediamine
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pH 5.0, 23°C
1.2 - 5600
o-Dianisidine
27 - 630
pyrogallol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21 - 4400
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
7400
3,3',5,5'-tetramethylbenzidine
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pH 5.0, 23°C
3900
ascorbate
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pH 5.0, 23°C
7200
chlorpromazine
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pH 5.0, 23°C
0.15 - 3200
H2O2
6000
N,N,N',N'-tetramethyl-p-phenylenediamine
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pH 5.0, 23°C
19 - 1200
o-Dianisidine
3200
pyrogallol
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pH 5.0, 23°C
0.014 - 0.48
tert-butyl hydroperoxide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.051
azide
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in 67 mM phosphate buffer, pH 7.0, at 30°C
0.0272 - 0.037
cyanide
0.0043
Isonicotinic acid hydrazide
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using o-dianisidine as substrate, in 50 mM phosphate buffer, pH 7.0, at 25°C
0.003 - 0.05
KCN
0.03 - 1.1
NaN3
0.0053
nicotinic acid hydrazide
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using o-dianisidine as substrate, in 50 mM phosphate buffer, pH 7.0, at 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6 - 30
3-Amino-1,2,4-triazole
0.0002 - 0.73
azide
0.003 - 1
cyanide
0.4 - 4.5
H2O2
0.00215 - 1.5
hydroxylamine
20
KCl
Oceanobacillus oncorhynchi subsp. incaldanensis
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catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C
10
MgCl2
Oceanobacillus oncorhynchi subsp. incaldanensis
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catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C
5
NaCl
Oceanobacillus oncorhynchi subsp. incaldanensis
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catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00904
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specific peroxidase activity, using isoniazid as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
0.0098
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specific peroxidase activity, using isoniazid as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
0.16
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catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C
0.18
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substrate o-dianisidine, mutant H290Q
0.208
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specific peroxidase activity, using NADH as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
0.212
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specific peroxidase activity, using NADH as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
0.25
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catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C
0.264
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specific peroxidase activity, using NADPH as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
0.272
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specific peroxidase activity, using NADPH as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
0.45
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
0.5
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substrate guajacol, mutant P151A
0.55
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peroxidase activity, using guaiacol as substrate, mutant enzyme N153A, in 50 mM phosphate buffer, pH 7.0, and 30°C
0.61
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crude enzyme, peroxidase activity, in 67 mM phosphate buffer, pH 7.0, at 25°C
0.8
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substrate o-dianisidine, mutant W341A
1
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peroxidase specific activity, using o-dianisidine as substrate, at pH 5.0 and 25°C
1.7
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after 2.79fold purification, peroxidase activity, in 67 mM phosphate buffer, pH 7.0, at 25°C
1.8
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substrate o-dianisidine, mutant D402N
2
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peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.0 and 25°C
2.1
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substrate o-dianisidine, mutant D402E
2.12
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specific peroxidase activity, using pyrogallol as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
2.29
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specific peroxidase activity, using pyrogallol as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
3.4
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crude extract, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C
3.8
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peroxidase activity, using o-dianisidine as substrate, wild type enzyme, in 50 mM phosphate buffer, pH 7.0, and 30°C
3.9
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crude extract, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C
4.1
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substrate guajacol, mutant D152W; substrate pyrogallol, mutant P151A
4.3
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peroxidase activity, using o-dianisidine as substrate, mutant enzyme N153D, in 50 mM phosphate buffer, pH 7.0, and 30°C
4.4
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crude extract, in 67 mM phosphate buffer (pH 7.0), at 25°C
4.8
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peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C
5
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crude extract, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1
5.3