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Literature summary for 1.11.1.21 extracted from

  • Nagy, J.M.; Cass, A.E.; Brown, K.A.
    Purification and characterization of recombinant catalase-peroxidase, which confers isoniazid sensitivity in Mycobacterium tuberculosis (1997), J. Biol. Chem., 272, 31265-31271.
    View publication on PubMed
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain UM255 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.96
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, using tert-butyl hydroperoxide as cosubstrate, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis
30
-
 H2O2 catalatic activity, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 2 * 80000, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + tert-butyl hydroperoxide
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + ?
-
 ?
H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 80000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, using tert-butyl hydroperoxide as cosubstrate, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis
2300
-
 H2O2 catalatic activity, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains approximately 0.5 heme per dimer Mycobacterium tuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium tuberculosis isoelectric focusing 3.8 3.7

General Information

General Information Comment Organism
physiological function catalase-peroxidase confers isoniazid (isonicotinic acid hydrazide) sensitivity in Mycobacterium tuberculosis Mycobacterium tuberculosis