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Literature summary for 1.11.1.21 extracted from

  • Ndontsa, E.N.; Moore, R.L.; Goodwin, D.C.
    Stimulation of KatG catalase activity by peroxidatic electron donors (2012), Arch. Biochem. Biophys., 525, 215-222.
    View publication on PubMed
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 ascorbate pH 5.0, 23°C Mycobacterium tuberculosis
0.2
-
 pyrogallol pH 5.0, 23°C Mycobacterium tuberculosis
0.49
-
 o-Dianisidine pH 5.0, 23°C Mycobacterium tuberculosis
0.7
-
 3,3',5,5'-tetramethylbenzidine pH 5.0, 23°C Mycobacterium tuberculosis
0.8
-
 H2O2 no electron donor present, pH 5.0, 23°C Mycobacterium tuberculosis
0.83
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 23°C Mycobacterium tuberculosis
1.1
-
 chlorpromazine pH 5.0, 23°C Mycobacterium tuberculosis
1.2
-
 N,N,N',N'-tetramethyl-p-phenylenediamine pH 5.0, 23°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
 Mycobacterium tuberculosis O2 + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis ? + H2O
-
 ?
3,3',5,5'-tetramethylbenzidine + H2O2
-
 Mycobacterium tuberculosis ? + H2O
-
 ?
ascorbate + H2O2
-
 Mycobacterium tuberculosis monodehydroascorbate + H2O
-
 ?
chlorpromazine + H2O2
-
 Mycobacterium tuberculosis ? + H2O
-
 ?
additional information presence of a low- and high-KM component for catalase activity at pH 5.0. Electron donors increase the apparent kcat for the low-KM component. During stimulated catalase activity, less than 0.008 equivalents of oxidized donor accumulate for every H2O2 consumed. A Fe(III)-O2 radical anion-like intermediate dominates during donor-stimulated catalatic turnover, and this intermediate converts directly to the ferric state upon depletion of H2O2 Mycobacterium tuberculosis ?
-
 ?
N,N,N',N'-tetramethyl-p-phenylenediamine + H2O2
-
 Mycobacterium tuberculosis ? + H2O
-
 ?
o-dianisidine + H2O2
-
 Mycobacterium tuberculosis ? + H2O
-
 ?
pyrogallol + H2O2
-
 Mycobacterium tuberculosis ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
520
-
 ascorbate pH 5.0, 23°C Mycobacterium tuberculosis
570
-
 H2O2 no electron donor present, pH 5.0, 23°C Mycobacterium tuberculosis
630
-
 pyrogallol pH 5.0, 23°C Mycobacterium tuberculosis
3300
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 23°C Mycobacterium tuberculosis
5220
-
 3,3',5,5'-tetramethylbenzidine pH 5.0, 23°C Mycobacterium tuberculosis
5600
-
 o-Dianisidine pH 5.0, 23°C Mycobacterium tuberculosis
7012
-
 N,N,N',N'-tetramethyl-p-phenylenediamine pH 5.0, 23°C Mycobacterium tuberculosis
7940
-
 chlorpromazine pH 5.0, 23°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
710
-
 H2O2 no electron donor present, pH 5.0, 23°C Mycobacterium tuberculosis
1200
-
 o-Dianisidine pH 5.0, 23°C Mycobacterium tuberculosis
3200
-
 pyrogallol pH 5.0, 23°C Mycobacterium tuberculosis
3900
-
 ascorbate pH 5.0, 23°C Mycobacterium tuberculosis
4000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 23°C Mycobacterium tuberculosis
6000
-
 N,N,N',N'-tetramethyl-p-phenylenediamine pH 5.0, 23°C Mycobacterium tuberculosis
7200
-
 chlorpromazine pH 5.0, 23°C Mycobacterium tuberculosis
7400
-
 3,3',5,5'-tetramethylbenzidine pH 5.0, 23°C Mycobacterium tuberculosis