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Literature summary for 1.11.1.21 extracted from
- Crystallization and preliminary X-ray analysis of the catalase-peroxidase KatG from Burkholderia pseudomallei (2002), Acta Crystallogr. Sect. D, 58, 2184-2186.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression | Burkholderia pseudomallei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme, hanging drop vapour diffusion method, 0.001 ml of 22 mg/ml protein in potassium phosphate, pH 7.0, mixed with equal volume of reservoir solution containing 16-20% PEG 4000, 20% 2-methyl-2,4-pentanediol, and 0.1 M sodium citrate, pH 5.6, 3-5 days, 20°C, X-ray diffraction strcuture determination and analysis at 1.8 A resolution, molecular replacement | Burkholderia pseudomallei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81600 | - |
x * 81600, sequence calculation | Burkholderia pseudomallei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 | Burkholderia pseudomallei | detoxification enzyme, protection against peroxides | O2 + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia pseudomallei | Q939D2 | gene katG, bifunctional enzyme KatG with catalase and peroxidase activities | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Burkholderia pseudomallei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2200 | - |
purified recombinant enzyme, catalase-specific activity | Burkholderia pseudomallei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 | - |
Burkholderia pseudomallei | O2 + H2O | - |
? | |
| H2O2 | detoxification enzyme, protection against peroxides | Burkholderia pseudomallei | O2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 81600, sequence calculation | Burkholderia pseudomallei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| catalase-peroxidase | - |
Burkholderia pseudomallei |
| CPX | - |
Burkholderia pseudomallei |
| KatG | - |
Burkholderia pseudomallei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Burkholderia pseudomallei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Burkholderia pseudomallei |
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