Protein Variants | Comment | Organism |
---|---|---|
E695A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
E695A/Y697A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
L690A/R691A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
L690A/R691A/E695A/Y697A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
R691A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
Y697A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2.4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
3.3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
3.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
4.5 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Mycobacterium tuberculosis | ? + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1020 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1050 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
3500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
4000 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
4800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
6800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
8300 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
8900 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
11500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
600 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
850 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
900 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
2500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
3100 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
4400 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis |