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Literature summary for 1.11.1.21 extracted from

  • Brown-Petersen, N.J.; Salin, M.L.
    Purification of a catalase-peroxidase from Halobacterium halobium: Characterization of some unique properties of the halophilic enzyme (1993), J. Bacteriol., 175, 4197-4202.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.21

Activating Compound

Activating Compound Comment Organism Structure
NaCl peroxidase activity is maximal at NaCl concentrations above 1 M, although catalase activity requires 2 M NaCl for optimal function Halobacterium salinarum

Inhibitors

Inhibitors Comment Organism Structure
H2O2
-
 Halobacterium salinarum
KCN catalase and peroxidase activities are effectively inhibited by KCN Halobacterium salinarum
additional information catalatic activity of the purified H catalase-peroxidase is not inhibited by incubation for 2.5 h with 20 mM 3 amino-1,2,4-triazole Halobacterium salinarum
NaN3 catalase and peroxidase activities are effectively inhibited by NaN3 Halobacterium salinarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 H2O2 peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
3.7
-
 H2O2 catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60300
-
 4 * 60300, estimated from SDS-PAGE Halobacterium salinarum
240000
-
 gel filtration Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Macrosphere GPC 300 column chromatography, hydroxylapatite column chromatography, and Sephadex G-150 gel filtration Halobacterium salinarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
 crude extract, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum
3.9
-
 crude extract, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
43.2
-
 after 12.7fold purification, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum
104.3
-
 after 29.6fold purification, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3'-diaminobenzidine + H2O2
-
 Halobacterium salinarum oxidized 3,3'-diaminobenzidine + H2O
-
 ?
4-aminoantipyrine + H2O2
-
 Halobacterium salinarum oxidized 4-aminoantipyrine + H2O
-
 ?
ascorbate + H2O2
-
 Halobacterium salinarum monodehydroascorbate + H2O
-
 ?
H2O2
-
 Halobacterium salinarum O2 + H2O
-
 ?
NADH + H2O2
-
 Halobacterium salinarum ?
-
 ?
NADPH + H2O2
-
 Halobacterium salinarum ?
-
 ?
o-dianisidine + H2O2
-
 Halobacterium salinarum oxidized o-dianisidine + H2O
-
 ?
reduced cytochrome c + H2O2
-
 Halobacterium salinarum oxidized cytochrome c + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 60300, estimated from SDS-PAGE Halobacterium salinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 peroxidase activity decreases rapidly above its maximum at 40°C Halobacterium salinarum
50
-
 catalase activity Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 90 at 90°C, the catalatic activity is 20% greater than at 25°C. Peroxidatic activity declines after incubation at temperatures higher than 40°C, although at 70°C nearly 50% of the activity at 25°C is retained Halobacterium salinarum

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 catalase activity Halobacterium salinarum
6.5 8 peroxidase activity Halobacterium salinarum

Cofactor

Cofactor Comment Organism Structure
heme heme content of 1.43 per tetramer Halobacterium salinarum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 NaN3 apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
0.05
-
 KCN apparent value, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum
0.05
-
 KCN apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
0.0675
-
 NaN3 apparent value, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum

pI Value

Organism Comment pI Value Maximum pI Value
Halobacterium salinarum isoelectric focusing
-
 3.8

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.4
-
 peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum H2O2
4.5
-
 catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum H2O2