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6.1.1.23: aspartate-tRNAAsn ligase

This is an abbreviated version!
For detailed information about aspartate-tRNAAsn ligase, go to the full flat file.

Word Map on EC 6.1.1.23

Reaction

ATP
+
L-aspartate
 +
tRNAAsx
=
AMP
 +
diphosphate
 +
L-aspartyl-tRNAAsx

Synonyms

aspartate tRNA synthetase, Aspartic acid translase, Aspartyl ribonucleic synthetase, aspartyl tRNA synthetase, aspartyl-transfer ribonucleate synthetase, Aspartyl-transfer ribonucleic acid synthetase, Aspartyl-transfer RNA synthetase, Aspartyl-tRNA synthetase, AspRS, EC 6.1.1.12, More, ND-AspRS, non-discriminating aspartyl-tRNA synthetase, nondicriminating AspRS, nondiscriminating aspartyl-tRNA synthetase, nondiscriminating AspRS, Synthetase, aspartyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.23 aspartate-tRNAAsn ligase

Cloned

Cloned on EC 6.1.1.23 - aspartate-tRNAAsn ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli trypA34 missense mutant transformed with heterologous ND-aspS gene
expressed in Escherichia coli
-
expressed in Escherichia coli B834 (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JF448 cells
expression in Escherichia coli
expression in JM101Tr cells
-
expression in YBC-603 cells
-
expression of C-terminally His-tagged enzyme in strain ADD1976
-
for expression in Escherichia coli cells
-
gene aspS, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes
-
gene aspS, phylogenetic tree, expression of His-tagged wild-type and mutant ND-AspRS in Escherichia coli strain trpA34, carrying a D60N mutation in trpA leading to tryptophan auxotrophy, co-expression of tRNAAsn leading to restoration of tryptophan prototrophy by missense suppression of the trpA34 mutant with heterologously in vivo formed Asp-tRNAAsn
gene DARS2, DNA and amino acid analysis of wild-type and mutant enzyme
-
overexpression in Escherichia coli
-
overexpression in Escherichia coli strain BL21(DE3)
-
overexpression of His-tagged AsnRS in Escherichia coli strain DH5alpha, the enzyme is toxic when heterologously overexpressed in Escherichia coli, because of sequestration of tRNAAsn as Asp-tRNAAsn, this toxicity is rescued upon coexpression of the Helicobacter pylori Asp/Glu-Adt, EC 6.3.5.6
-
overproduced from the cloned gene in Pseudomonas aeruginosa
-
recombinant expression of the N-terminally His-tagged AspRS
the N-terminal anticodon-binding domain of non-discriminating aspartyl-tRNA synthetase is expressed in Escherichia coli BL21(DE3) cells
-