6.1.1.23: aspartate-tRNAAsn ligase

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For detailed information about aspartate-tRNAAsn ligase, go to the full flat file.

Reaction

Synonyms

aspartate tRNA synthetase, Aspartic acid translase, Aspartyl ribonucleic synthetase, aspartyl tRNA synthetase, aspartyl-transfer ribonucleate synthetase, Aspartyl-transfer ribonucleic acid synthetase, Aspartyl-transfer RNA synthetase, Aspartyl-tRNA synthetase, AspRS, EC 6.1.1.12, More, ND-AspRS, non-discriminating aspartyl-tRNA synthetase, nondicriminating AspRS, nondiscriminating aspartyl-tRNA synthetase, nondiscriminating AspRS, Synthetase, aspartyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.23 aspartate-tRNAAsn ligase

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Results	in table
1	Activating Compound
27069	AA Sequence
1	CAS Registry Number
25	Cloned(Commentary)
14	Cofactor
7	Crystallization (Commentary)
2	Disease/ Diagnostics
1	General Information
1	General Stability
2	Inhibitors
9	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
4	Localization
9	Metals/Ions
6	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
43	Organism
5	Pathway
10	PDB ID
6	pH Optimum
1	Posttranslational Modification
28	Protein Variants
13	Purification (Commentary)
4	Reaction
3	Reaction Type
38	Reference
3	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
80	Substrates and Products (Substrate)
8	Subunits
65	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
27	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 6.1.1.23 - aspartate-tRNAAsn ligase

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SYSTEMATIC NAME

IUBMB Comments

L-aspartate:tRNAAsx ligase (AMP-forming)

When this enzyme acts on tRNAAsp, it catalyses the same reaction as EC 6.1.1.12, aspartate---tRNA ligase. It has, however, diminished discrimination, so that it can also form aspartyl-tRNAAsn. This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon [1]. This accounts for the ability of this enzyme in, for example, Thermus thermophilus, to recognize both tRNAAsp (GUC anticodon) and tRNAAsn (GUU anticodon). The aspartyl-tRNAAsn is not used in protein synthesis until it is converted by EC 6.3.5.6, asparaginyl-tRNA synthase (glutamine-hydrolysing), into asparaginyl-tRNAAsn.