6.1.1.23: aspartate-tRNAAsn ligase

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For detailed information about aspartate-tRNAAsn ligase, go to the full flat file.

Reaction

Synonyms

aspartate tRNA synthetase, Aspartic acid translase, Aspartyl ribonucleic synthetase, aspartyl tRNA synthetase, aspartyl-transfer ribonucleate synthetase, Aspartyl-transfer ribonucleic acid synthetase, Aspartyl-transfer RNA synthetase, Aspartyl-tRNA synthetase, AspRS, EC 6.1.1.12, More, ND-AspRS, non-discriminating aspartyl-tRNA synthetase, nondicriminating AspRS, nondiscriminating aspartyl-tRNA synthetase, nondiscriminating AspRS, Synthetase, aspartyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.23 aspartate-tRNAAsn ligase

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Results	in table
1	Activating Compound
27069	AA Sequence
1	CAS Registry Number
25	Cloned(Commentary)
14	Cofactor
7	Crystallization (Commentary)
2	Disease/ Diagnostics
1	General Information
1	General Stability
2	Inhibitors
9	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
4	Localization
9	Metals/Ions
6	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
43	Organism
5	Pathway
10	PDB ID
6	pH Optimum
1	Posttranslational Modification
28	Protein Variants
13	Purification (Commentary)
4	Reaction
3	Reaction Type
38	Reference
3	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
80	Substrates and Products (Substrate)
8	Subunits
65	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
27	Turnover Number [1/s]

Purification

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Purification on EC 6.1.1.23 - aspartate-tRNAAsn ligase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Methanothermobacter thermautotrophicus

O26328

440091

Ni-NTA column chromatography

Staphylococcus aureus

-

744899

Ni-NTA column chromatography, and gel filtration

Helicobacter pylori

-

728743

nickel-affinity column chromatography

Bdellovibrio bacteriovorus

Q6MI59

746252

recombinant enzyme

2 entries

recombinant enzyme from Escherichia coli strain BL21(DE3)

Sulfurisphaera tokodaii

-

671055

recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydroxyapatite chromatography

Sulfurisphaera tokodaii

-

671133

recombinant His-tagged AsnRS from Escherichia coli strain DH5alpha by nickel affinity chromatography

Helicobacter pylori

-

672190

recombinant His-tagged AspRS, cleavage of the N-terminal His-tag

Entamoeba histolytica

C4LZN0

705605

recombinant His-tagged enzyme from strain ADD1976 by nickel affinity chromatography

Pseudomonas aeruginosa

-

675112

recombinant His-tagged wild-type and mutant ND-AspRS in Escherichia coli strain trpA34 by nickel affinity chromatography

Halobacterium salinarum

O07683

677106

the transamidosome complex is isolated by preparative gel filtration on a Superdex G200 column

Thermus thermophilus

-

701525

recombinant enzyme

Thermus thermophilus

-

440089

recombinant enzyme

Thermococcus kodakarensis

-

440088