6.1.1.23: aspartate-tRNAAsn ligase

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For detailed information about aspartate-tRNAAsn ligase, go to the full flat file.

Reaction

Synonyms

aspartate tRNA synthetase, Aspartic acid translase, Aspartyl ribonucleic synthetase, aspartyl tRNA synthetase, aspartyl-transfer ribonucleate synthetase, Aspartyl-transfer ribonucleic acid synthetase, Aspartyl-transfer RNA synthetase, Aspartyl-tRNA synthetase, AspRS, EC 6.1.1.12, More, ND-AspRS, non-discriminating aspartyl-tRNA synthetase, nondicriminating AspRS, nondiscriminating aspartyl-tRNA synthetase, nondiscriminating AspRS, Synthetase, aspartyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.23 aspartate-tRNAAsn ligase

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Results	in table
1	Activating Compound
27069	AA Sequence
1	CAS Registry Number
25	Cloned(Commentary)
14	Cofactor
7	Crystallization (Commentary)
2	Disease/ Diagnostics
1	General Information
1	General Stability
2	Inhibitors
9	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
4	Localization
9	Metals/Ions
6	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
43	Organism
5	Pathway
10	PDB ID
6	pH Optimum
1	Posttranslational Modification
28	Protein Variants
13	Purification (Commentary)
4	Reaction
3	Reaction Type
38	Reference
3	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
80	Substrates and Products (Substrate)
8	Subunits
65	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
27	Turnover Number [1/s]

Crystallization

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Crystallization on EC 6.1.1.23 - aspartate-tRNAAsn ligase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified AspRS, sitting drop vapour diffusion method, 0.0002 ml of 9 mg/ml protein is mixed with 0.0002 ml of reservoir solution containing 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, and 23% w/v PEG 3350, X-ray diffraction structure determination and analysis at 2.8 A resolution

Entamoeba histolytica

C4LZN0

705605

N-terminal anticodon-binding domain of non-discriminating aspartyl-tRNA synthetase, hanging drop vapor diffusion method, using 30% (w/v) PEG 4000, 100 mM ammonium sulfate, 100 mM sodium citrate at pH 5.6

Helicobacter pylori

-

728743

purified recombinant enzyme, 5-8 mg/ml in 20 mM Tris–HCl buffer, pH 7.0, hanging-drop vapour-diffusion method, 26°C, from 0.002 ml protein solution is mixed with 0.002 ml of reservoir solution containing 100 mM sodium HEPES buffer, pH 7.5, containing 100 mM NaCl and 1.6 M (NH4)2SO4, equilibration against 0.7 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement

Sulfurisphaera tokodaii

-

671133

purified recombinant enzyme, X-ray diffraction structure determination and analysis at 2.3 A resolution

Sulfurisphaera tokodaii

-

671055

hanging drop technique, 24°C, 5 days in Crystal Screen I or II, solutions containing either polyethylene glycol or ethylene glycol

Thermococcus kodakarensis

-

440088

a complete dataset of the transamidosome is collected to 3 A resolution

Thermus thermophilus

-

701525

purified 520 kDa transamidosome complex formed by two dimeric nondiscriminating-AspRSs, two trimeric GatCABs, and four tRNAsAsn molecules, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement

Thermus thermophilus

-

714830